Описание: Handbook of Molecular Microbial Ecology I: Metagenomics and Complementary Approaches is the first comprehensive reference covering the various metagenomics in a large variety of habitats, which could not previously have been analysed without metagenomic methodology.
Описание: Metagenomics has revolutionized microbiology and many associated health and environmental fields. This is the first comprehensive treatise covering the various omics in a large variety of habitats, which could not previously have been analysed without metagenomic methodology.
This book provides comprehensive, authoritative discussions about microbial communities in environments that are rich in hydrocarbons, crude oil or lipids. It encompasses natural environments, such as tar sands, oil seeps and reservoirs, as well as habitats where methane is produced. Equally, the book deals with habitats that have been influenced by human activity, including oil-contaminated soils, aquifers, coast and seas. The book opens with a series of chapters considering the contemporary approaches used to investigate microbial communities.
Автор: Streit Wolfgang R., Daniel Rolf Название: Metagenomics: Methods and Protocols ISBN: 1493982745 ISBN-13(EAN): 9781493982745 Издательство: Springer Рейтинг: Цена: 12577.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This second edition explores up-to-date tools in various function-based technologies currently used in metagenomics. The chapters in this book discuss all of the working steps involved in these technologies, such as: DNA isolation from soils and marine samples followed by the construction and screening of libraries for diverse enzymes and biomolecules; current methods used to isolate DNA and construct large- and small-insert libraries from terrestrial and marine habitats; methods for establishing metagenome libraries in none-E.coli hosts; new molecular tools used for function-driven mining of metagenomic DNA; and screening protocols for a wide array of different genes encoding enzymes with relevance to biotechnology and ecology. Metagenomics: Methods and Protocols, Second Edition also provides detailed screening protocols for phosphatases, poly-hydroxyalkanoate, metabolism-related enzymes, stereoselective hydrolases, and microbial signals for the discovery of secondary metabolites.
Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Metagenomics: Methods and Protocols, Second Edition is a collection of up-to-date metagenome protocols and tools for the recovery of numerous major types of biocatalysts, and allows researchers to easily setup these screens in any microbiology laboratory.
This book provides comprehensive, authoritative discussions about microbial communities in environments that are rich in hydrocarbons, crude oil or lipids. It encompasses natural environments, such as tar sands, oil seeps and reservoirs, as well as habitats where methane is produced. Equally, the book deals with habitats that have been influenced by human activity, including oil-contaminated soils, aquifers, coast and seas. The book opens with a series of chapters considering the contemporary approaches used to investigate microbial communities.
This book provides comprehensive, authoritative discussions about microbial communities in environments that are rich in hydrocarbons, crude oil or lipids. It encompasses natural environments, such as tar sands, oil seeps and reservoirs, as well as habitats where methane is produced. Equally, the book deals with habitats that have been influenced by human activity, including oil-contaminated soils, aquifers, coast and seas. The book opens with a series of chapters considering the contemporary approaches used to investigate microbial communities.
Автор: Wolfgang R. Streit; Rolf Daniel Название: Metagenomics ISBN: 1493961233 ISBN-13(EAN): 9781493961238 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Metagenomics has proven to be a powerful tool for exploring the ecology, metabolic profiling, and comparison of complex microbial communities as well as its important applications in the mining of metagenomes for genes encoding novel biocatalysts and drug molecules for bioindustries. In Metagenomics: Methods and Protocols, expert researches provide an overview and introduction to basic methods commonly used in laboratories that have a strong background in microbial metagenomics. The book attempts to address all of the working steps involved in this crucial field, beginning with DNA isolation from soils and marine samples and continuing with the construction and screening of libraries, along with key advise involving bioinformatic tools available to analyze large metagenomic sequence data sets. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Metagenomics: Methods and Protocols serves as a very complete guide to available screening protocols for all major biocatalysts in order to allow for the easy setup of these screens in any microbiology lab.
Автор: Vitantonio Pantaleo; Michela Chiumenti Название: Viral Metagenomics ISBN: 1493992570 ISBN-13(EAN): 9781493992577 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Поставка под заказ.
Описание: This volume explores the use of viral metagenomics to diagnose known viruses for plant and food production, human and animal health, and identifying viral vectors like insects. The chapters in this book cover topics, such as sRNAs isolation from tissues of grapevines and woody plants, high-resolution screening of arthropod and plant viral communities, identifying new pathogens in fish, detecting viruses in mycorrhizal fungi and their orchid host, and insect virus discovery through metagenomic and cell culture-based approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, Viral Metagenomics: Methods and Protocols is a valuable resource for researchers and specialists who are interested in learning more about this evolving field.
Автор: Trevor C. Charles; Mark R. Liles; Angela Sessitsch Название: Functional Metagenomics: Tools and Applications ISBN: 3319870963 ISBN-13(EAN): 9783319870960 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Поставка под заказ.
Описание: In this book, the latest tools available for functional metagenomics research are described.This research enables scientists to directly access the genomes from diverse microbial genomes at one time and study these “metagenomes”. Using the modern tools of genome sequencing and cloning, researchers have now been able to harness this astounding metagenomic diversity to understand and exploit the diverse functions of microorganisms. Leading scientists from around the world demonstrate how these approaches have been applied in many different settings, including aquatic and terrestrial habitats, microbiomes, and many more environments. This is a highly informative and carefully presented book, providing microbiologists with a summary of the latest functional metagenomics literature on all specific habitats.
Описание: This book summarizes the various areas of research in metagenomics and their potential applications in medicine, the environment and biotechnology.
Автор: Wolfgang Streit; Rolf Daniel Название: Metagenomics ISBN: 1493966898 ISBN-13(EAN): 9781493966899 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. Construction of Small-Insert and Large-Insert Metagenomic Libraries Carola Simon and Rolf Daniel 2. Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies Dominik Schneider, Franziska Wemheuer, Birgit Pfeiffer, and Bernd Wemheuer 3. Construction and Screening Marine Metagenomic Large Insert Libraries Nancy Weiland-Brдuer, Daniela Langfeldt, and Ruth A. Schmitz 4. Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment Mikkel A. Glaring, Jan K. Vester, and Peter Stougaard 5. DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms Eleanor Jameson, Martin Taubert, Sara Coyotzi, Yin Chen, Цzge Eyice, Hendrik Schдfer, J. Colin Murrell, Josh D. Neufeld, and Marc G. Dumont 6. Assessing Bacterial and Fungal Diversity in the Plant's Endosphere Bernd Wemheuer and Franziska Wemheuer 7. Shotgun Metagenomic Sequencing analysis of Soft-Rot Enterobacteriaceae in Polymicrobial Communities James Doonan, Sandra Denman, James E. McDonald, and Peter M. Golyshin 8. Cloning and Expression of Metagenomic DNA in Streptomyces lividans and its Subsequent Fermentation for Optimized Production Yuriy Rebets, Jan Kormanec, Andriy Lutzhetskyy, Kristel Bernaerts, and Jozef Annй 9. Degradation Network Reconstruction Guided by Metagenomic Data Rafael Bargiela and Manuel Ferrer 10. Novel Tools for the Functional Expression of Metagenomics DNA Nadine Katzke, Andreas Knapp, Anita Loeschcke, Thomas Drepper, and Karl-Erick Jaeger 11. A Microtiter Plate-Based Assay to Screen for Active and Stereoselective Hydrolytic Enzymes in Enzyme Libraries Dominique Bцttcher, Patrick Zдgel, Marlen Schmidt, and Uwe T. Bornscheuer 12. Screening for Cellulase Encoding Clones in Metagenomics Libraries Nele Ilmberger and Wolfgang R. Streit 13. Liquid Phase Multiplex High-Throughput Screening of Metagenomic Libraries using p-Nitrophenyl-Linked Substrates for Accessory Lignocellulosic Enzymes Mariette Smart, Robert J. Huddy, Don A. Cowan, and Marla Trindade 14. Screening Glycosyltransferases for Polyphenol Modifications Nele Ilmberger and Ulrich Rabausch 15. Methods for the Isolation of Genes Encoding Novel PHA Matabolism Enzymes from Complex Microbial CommunitiesJiujun Cheng, Ricardo Nordeste, Maria A. Trainer, and Trevor C. Charles 16. Function-Based Metagenomic Library Screening and Heterologous Expression Strategy for Genes Encoding Phosphatase Activity Genis A. Castillo Villamizar, Heiko Nacke, and Rolf Daniel 17. Activity-Based Screening of Metagenomics Libraries for Hydrogenase Enzymes Nicole Adam and Mirjam Perner 18. Screening for N-AHSL-Based-Signaling Interfering Enzymes Stйphane Uroz and Phil M. Oger 19. Mining Microbial Signals for Enhanced Biodiscovery of Secondary Metabolites F. Jerry Reen, Jose A. Gutiйrrez-Barranquero, and Fergal O'Gara
Описание: The book serves as an amalgamation of knowledge and principles used in the area of systems and synthetic biology, and targets inter-disciplinary research groups.
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