The mRNA Metabolism in Human Disease, Lu?sa Rom?o

Автор: Lu?sa Rom?o
Название: The mRNA Metabolism in Human Disease
ISBN: 3030199657 ISBN-13(EAN): 9783030199654
Издательство: Springer
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Описание: The eukaryotic gene expression pathway involves a number of interlinked steps, with messenger RNA (mRNA) being the key intermediate. The precursor mRNA is transcribed from DNA, processed by removal of introns and addition of the cap structure and the poly(A) tail. The mature mRNA is then exported to the cytoplasm where it is translated into protein and finally degraded. In this process, mRNA is associated with RNA-binding proteins forming ribonucleoprotein complexes, whose protein content evolves throughout the lifetime of the mRNA. While the complexity of eukaryotic gene expression allows the production of proteins to be controlled at many levels, it also makes the process vulnerable to errors. Although eukaryotic cells have evolved elaborate mRNA quality control mechanisms that ensure the fidelity of gene expression, some defects are not detected, thus affecting mRNA metabolism. This condition plays a fundamental role in the pathogenesis of several disease processes, such as neurodegeneration and oncogenesis. Besides, exciting recent data have shown that cellular RNAs can be modified post-transcriptionally via dynamic and reversible chemical modifications, the so-called epitranscriptome. These modifications can alter mRNA structure, being able to modulate different steps of the mRNA metabolism that can be associated with various human diseases, such as systemic lupus erythematosus and cancer. This book provides a collection of novel studies and hypotheses aimed to define the pathophysiological consequences of altered mRNA metabolism events in human cells, and is written for a wide spectrum of readers in the field of gene expression regulation. The last chapter highlights how the discovery of disease-causing defects (or modifications) in mRNA can provide a variety of therapeutic targets that can be used for the development of new RNA-based therapeutics. Hopefully, it may also contribute to inspire the drug-developing scientific community.

Автор: Fabrice Lejeune
Название: Nonsense-Mediated mRNA Decay in Human Disease
ISBN: 0128044683 ISBN-13(EAN): 9780128044681
Издательство: Elsevier Science
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Описание: . Nonsense Mutation Correction in Human Diseases: An Approach for Targeted Medicine provides an introduction on genetic diseases, discusses the prevalence of nonsense mutations, the consequences of a nonsense mutation for the expression of the mutant gene, and the presentation of the nonsense-mediated mRNA decay (NMD). . It presents the mechanism of action and rationale associated with each strategy to correct nonsense mutations with the results of clinical trials to further support this basis. In addition, the book shows how it may be possible to combine several of these strategies to ultimately improve the efficiency of correction, also suggesting the future goals and objectives to improve treatment modalities in this evolving sphere of personalized medicine.

Автор: Dey Aparajita
Название: Cytochrome P450 2e1: Its Role in Disease and Drug Metabolism
ISBN: 9401782024 ISBN-13(EAN): 9789401782029
Издательство: Springer
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Цена: 20962.00 р.
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Описание: The book deals with various clinical aspects of cytochrome P450 2E1 (CYP2E1) which is a potent source for oxidative stress. Oxidative stress is critical for pathogenesis of diseases and CYP2E1 is a major contributor for oxidative stress. Several clinical disorders are associated with changes in regulation of CYP2E1 and the consequent abnormalities which include alcoholic liver disease, alcoholic pancreatitis, carcinogenesis, non-alcoholic fatty liver disease, non-alcoholic steatohepatitis, obesity, hepatitis C virus infection, reproductive organ toxicity, hepatocellular and cholestatic liver cirrhosis, inhibition of bone repair, cross-tolerance in smokers and people treated with nicotine, disorders of central nervous system, changes in metabolism of protoxicants in the circulatory system and susceptibility to human papillomavirus infection. Hence, CYP2E1 emerges as a new and potent player in aggravating injury and furthering disease complications.

Автор: Tian, Bin
Название: Mrna 3` End Processing And Stability,654
ISBN: 012823573X ISBN-13(EAN): 9780128235737
Издательство: Elsevier Science
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mRNA 3' End Processing and Stability, Volume 655 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics. Each chapter is written by an international board of authors.

Автор: Shi, Yongsheng.
Название: MRNA processing :
ISBN: 1493972030 ISBN-13(EAN): 9781493972036
Издательство: Springer
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Описание: This detailed volume assembles a number of the most commonly used and state-of-the-art methodologies in the revolutionary field of mRNA processing in order to aid researchers in choosing the best method for their specific problems.

Автор: Robert E. Rhoads
Название: Synthetic mRNA
ISBN: 149398098X ISBN-13(EAN): 9781493980987
Издательство: Springer
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Цена: 15372.00 р.
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Описание: This volume presents detailed laboratory protocols for in vitro synthesis of mRNA with favorable properties, its introduction into cells by a variety of techniques, and the measurement of physiological and clinical consequences such as protein replacement and cancer immunotherapy. Synthetic techniques are described for structural features in mRNA that provide investigational tools such as fluorescence emission, click chemistry, photo-chemical crosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reduced activation of the innate immune response. Protocols are described for clinical applications such as large-scale transfection of dendritic cells, production of GMP-grade mRNA, redirecting T cell specificity, and use of molecular adjuvants for RNA vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resource for both laboratory investigators and clinicians interested in this powerful and rapidly evolving technology.

Автор: Shireen R. Lamand?
Название: mRNA Decay
ISBN: 1493975390 ISBN-13(EAN): 9781493975396
Издательство: Springer
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Цена: 16769.00 р.
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Описание: This detailed volume explores the latest methods that can be used to probe mRNA decay pathways and identify mRNA-binding protein targets as well as miRNA targets.

Автор: Shireen R. Lamand?
Название: mRNA Decay
ISBN: 1493985159 ISBN-13(EAN): 9781493985159
Издательство: Springer
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Описание: This detailed volume explores the latest methods that can be used to probe mRNA decay pathways and identify mRNA-binding protein targets as well as miRNA targets. Subjects include metabolic labelling and RNAseq methods for determining RNA decay rates, approaches for discovering RNA-binding protein targets, bioinformatics, miRNA targets and novel components of the miRNA-directed decay pathway, and recently developed approaches for studying nonsense-mediated mRNA decay, among other areas. Written for the highly popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Автор: Yongsheng Shi
Название: mRNA Processing
ISBN: 1493984152 ISBN-13(EAN): 9781493984152
Издательство: Springer
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Описание: This detailed volume assembles a number of the most commonly used and state-of-the-art methodologies in the revolutionary field of mRNA processing in order to aid researchers in choosing the best method for their specific problems.

Автор: Marlene Oeffinger; Daniel Zenklusen
Название: The Biology of mRNA: Structure and Function
ISBN: 3030314332 ISBN-13(EAN): 9783030314330
Издательство: Springer
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Описание:

Автор: Robert E. Rhoads
Название: Synthetic mRNA
ISBN: 1493936239 ISBN-13(EAN): 9781493936236
Издательство: Springer
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Цена: 19564.00 р.
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Описание: This volumepresents detailed laboratory protocols for invitro synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy.

Автор: Klemens J. Hertel
Название: Spliceosomal Pre-mRNA Splicing
ISBN: 149396044X ISBN-13(EAN): 9781493960446
Издательство: Springer
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Описание:

Part I: Introductory Chapters

1. The Pre-mRNA Splicing Reaction

Somsakul Wongpalee and Shalini Sharma

2. Diversity and Evolution of Spliceosomal Systems

Scott William Roy and Manuel Irimia

3. Mechanisms of Spliceosomal Assembly

Ni-ting Chiou and Kristen W. Lynch

4. Alternative Pre-mRNA Splicing

Stacey D. Wagner and J. Andrew Berglund

5. Regulation of Alternative Pre-mRNA Splicing

Miguel B. Coelho and Christopher W.J. Smith

6. Introduction to Co-Transcriptional RNA Splicing

Evan C. Merkhofer, Peter Hu, and Tracy L. Johnson

7. Chromatin and Splicing

Nazmul Haque and Shalini Oberdoerffer

Part II: Methods Chapters

8. Preparation of Splicing Competent Nuclear Extracts

Chiu-Ho T. Webb and Klemens J. Hertel

9. Preparation of Yeast Whole Cell Splicing Extract

Elizabeth A. Dunn and Stephen D. Rader

10. Efficient Splinted Ligation of Synthetic RNA Using RNA Ligase

Martha R. Stark and Stephen D. Rader

11. In Vitro Assay of Pre-mRNA Splicing in Mammalian Nuclear Extract

Maliheh Movassat, William F. Mueller, and Klemens J. Hertel

12. Kinetic Analysis of In Vitro Pre-mRNA Splicing in HeLa Nuclear Extract

William F. Mueller and Klemens J. Hertel

13. In Vitro Systems for Coupling RNAP II Transcription to Splicing and Polyadenylation

Eric G. Folco and Robin Reed

14. Isolation and Accumulation of Spliceosomal Assembly Intermediates

Janine O. Ilagan and Melissa S. Jurica

15. Complementation of U4 snRNA in S. cerevisiae Splicing Extracts for Biochemical Studies of snRNP Assembly and Function

Martha R. Stark and Stephen D. Rader

16. Expression and Purification of Splicing Proteins from Mammalian Cells

Eric Allemand and Michelle L. Hastings

17. Single Molecule Approaches for Studying Spliceosome Assembly and Catalysis

Eric G. Anderson and Aaron A. Hoskins

18. Cell-Based Splicing of Minigenes

Sarah A. Smith and Kristen W. Lynch

19. Quantifying the Ratio of Spliceosome Components Assembled on Pre-mRNA

Noa Neufeld, Yehuda Brody, and Yaron Shav-Tal

20. Antisense Methods to Modulate Pre-mRNA Splicing

Joonbae Seo, Eric W. Ottesen, and Ravindra N. Singh

21. Using Yeast Genetics to Study Splicing Mechanisms

Munshi Azad Hossain and Tracy L. Johnson

22. Medium Throughput Analysis of Alternative Splicing by Fluorescently Labeled RT-PCR

Ryan Percifield, Daniel Murphy, and Peter Stoilov

23. Chromatin Immunoprecipitation Approaches to Determine Co-Transcriptional Nature of Splicing

Nicole I. Bieberstein, Korinna Straube, and Karla M. Neugebauer

24. Computational Approaches to Mine Publicly Available Databases

Rodger B. Voelker, William A. Cresko, and J. Andrew Berglund

25. Approaches to Link RNA Secondary Structures with Splicing Regulation

Mireya Plass and Eduardo Eyras

26. Methods to Study Splicing from High-Throughput RNA Sequencing Data

Gael P. Alamancos, Eneritz Agirre, and Eduardo Eyras

27. Global Protein-RNA Interaction Mapping at Single Nucleotide Resolution by iCLIP-Seq

Chengguo Yao, Lingjie Weng, and Yongsheng Shi

28. Predicting Alternative Splicing

Yoseph Barash and Jorge Vaquero Garcia