Автор: Ian M. Clark Название: Matrix Metalloproteinase Protocols ISBN: 1603272984 ISBN-13(EAN): 9781603272988 Издательство: Springer Рейтинг: Цена: 14673.00 р. 20962.00-30% Наличие на складе: Есть (1 шт.) Описание: Detailing proven laboratory techniques for the study of MMPs, this book covers the methods including those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a variety of circumstances.
Автор: Turksen Название: Human Embryonic Stem Cell Protocols ISBN: 1607613689 ISBN-13(EAN): 9781607613688 Издательство: Springer Рейтинг: Цена: 14673.00 р. 20962.00-30% Наличие на складе: Есть (1 шт.) Описание: Controversy surrounds the study of human embryonic stem cells. In this book, respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells.
Автор: Fulga Tudor A., Knapp David J. H. F., Ferry Quentin R. V. Название: Crispr Guide RNA Design: Methods and Protocols ISBN: 1071606867 ISBN-13(EAN): 9781071606865 Издательство: Springer Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner.
Автор: Hui Li; Justin Elfman Название: Chimeric RNA ISBN: 1493999060 ISBN-13(EAN): 9781493999064 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Нет в наличии.
Описание: This book provides numerous methods for identification, validation, and functional characterization of chimeric RNAs, herein described as any transcript which contains the nucleotide sequence of two distinct parental genes.
Автор: Basha, Mahin Название: CRISPR Guide RNA Design ISBN: 1071606891 ISBN-13(EAN): 9781071606896 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner.
Автор: Christina Waldsich Название: RNA Folding ISBN: 1627036660 ISBN-13(EAN): 9781627036665 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Focusing on in vitro and intracellular RNA structure formation, RNA Folding: Methods and Protocols provides a comprehensive collection of experimental protocols which are suitable to dissect RNA folding pathways and to characterize the structure of RNA folding intermediates at nucleotide or even atomic resolution.
Автор: Khalaf Yasmin Название: DNA and RNA Extraction: Methods and Protocols ISBN: 3659495034 ISBN-13(EAN): 9783659495038 Издательство: Неизвестно Цена: 12949.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Автор: Zdravka Medarova Название: RNA Imaging ISBN: 1493949748 ISBN-13(EAN): 9781493949748 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed volume will focus on the phenomenon of RNA interference by providing comprehensive coverage of various techniques for in vivo micro/siRNA imaging including the design and synthesis of specific imaging agents and tools, the development of imaging methodologies, and their interpretation. An essential element in the development and optimization of these therapies is the ability to measure the bioavailability and functionality of the RNA/oligonucleotide molecule after administration into the body. Noninvasive imaging provides the necessary set of tools to accomplish this in authentic physiologic environments and across time. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, RNA Imaging: Methods and Protocols serves physicians, scientists, and graduate students who are either new to the field of RNA-based imaging and its associated therapeutic applications or who wish to be apprised of recent advances in the state of the art.
Автор: Hui Li; Justin Elfman Название: Chimeric RNA ISBN: 1493999036 ISBN-13(EAN): 9781493999033 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Нет в наличии.
Описание: This book provides numerous methods for identification, validation, and functional characterization of chimeric RNAs, herein described as any transcript which contains the nucleotide sequence of two distinct parental genes. Beginning with identification and characterization through bioinformatics tools, the book continues with experimental validation using RT-PCR, hybridization methods for RNA, and the use of mass spectrometry data for fusion protein validation, as well as functional and mechanistic studies of chimeric RNAs and translational applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Chimeric RNA: Methods and Protocols is an ideal guide to this expansive field and an inspiration to researchers seeking to further our knowledge of these remarkable features of the transcriptome.
Автор: Ren-Jang Lin Название: RNA-Protein Complexes and Interactions ISBN: 1493935895 ISBN-13(EAN): 9781493935895 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Characterization of RNA-Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and SRSF2
Lindsey Skrdlant and Ren-Jang Lin
2. Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification)
Je-Hyun Yoon and Myriam Gorospe
3. Biotin-Streptavidin Affinity Purification of RNA-Protein Complexes Assembled In Vitro
Shuai Hou, Lei Shi, and Haixin Lei
4. Detecting RNA-Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting
Xuexiu Zheng, Sunghee Cho, Heegyum Moon, Tiing Jen Loh, Hana Jang, and Haihong Shen
5. Purification of RNA-Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture
Naoyuki Kataoka
6. Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts
Keith T. Gagnon
7. In Vitro Analysis of Ribonucleoprotein Complex Remodeling and Disassembly
Hua-Lin Zhou and Hua Lou
8. Single-Turnover Kinetics of Methyl Transfer to tRNA by Methyltransferases
Ya-Ming Hou
9. Purification and Functional Reconstitution of Box H/ACA Ribonucleoprotein Particles
Chao Huang, Guowei Wu, and Yi-Tao Yu
10. Northwestern Blot Analysis: Detecting RNA-Protein Interaction After Gel Separation of Protein Mixture
Shangbing Zang and Ren-Jang Lin
11. Measuring mRNA Translation by Polysome Profiling
Marek Kudla and Fedor V. Karginov
12. Genome-Wide Profiling of RNA-Protein Interactions Using CLIP-Seq
Cheryl Stork and Sika Zheng
13. Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichment
Sarah F. Mitchell and Roy Parker
14. Ribo-Proteomics Approach to Profile RNA-Protein and Protein-Protein Interaction Networks
Hsin-Sung Yeh, Jae-Woong Chang, and Jeongsik Yong
15. Detection of Protein-Protein Interaction Within an RNA-Protein Complex Via Unnatural-Amino-Acid-Mediated Photochemical Crosslinking
Fu-Lung Yeh, Luh Tung, and Tien-Hsien Chang
16. Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX
Jiehua Zhou and John J. Rossi
17. mCarts: Genome-Wide Prediction of Clustered Sequence Motifs as Binding Sites for RNA Binding Proteins
Sebastien M. Weyn-Vanhentenryck and Chaolin Zhang
18. Design
Автор: Sarkar Devanand Название: Non-Alcoholic Steatohepatitis ISBN: 1071621270 ISBN-13(EAN): 9781071621271 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume contains a comprehensive collection of laboratory protocols used by researchers to analyze varied aspects of non-alcoholic steatohepatitis (NASH).
Автор: Steger Gerhard Название: Dnazymes ISBN: 1071620460 ISBN-13(EAN): 9781071620465 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Acknowledgement...Preface...Table of Contents...Contributing Authors... Part I SELEX1. A Protocol for Gold Nanoparticle-Assisted Aptamer Selection for a Small Molecule Porphyrin to Develop DNAzymeWenjing Li, Yanwei Cao, and Renjun Pei 2. Selection of Aptamer for N-methyl Mesoporphyrin IX to Develop Porphyrin Metalation DNAzymeLuyan Yang, Yanwei Cao, and Renjun Pei 3. PCR Methods for the Generation of Catalytic ssDNADeni Szokoli, Kristian K. Le Vay, and Hannes Mutschler Part II Design - Kinetics/Performance/Turnover 4. Design of a DNAzyme: Prediction of mRNA Regions Accessible to a DNAzymeGerhard Steger and Julian Victor 5. Fluorescence-Based Kinetic Measurements for RNA-Cleaving DNAzymesHannah Rosenbach and Gerhard Steger 6. Stability and Activity of the 10-23 DNAzyme under Molecular Crowding ConditionsNina Kirchgдssler, Hannah Rosenbach, and Ingrid Span 7. Quantifying the Number and Affinity of Mn2+-Binding Sites with EPR SpectroscopyChristine Wuebben and Olav Schiemann Part III Biophysical Characterization of DNAzymes 8. Obtaining Crystals of Nucleic Acids in Complex with the Protein U1A Using the Soaking MethodHannah Rosenbach and Ingrid Span 9. Crystallization and Structural Determination of 8-17 DNAzymeHehua Liu, Song Mao, Jia Sheng, and Jianhua Gan 10. Solution NMR Spectroscopy as a Tool to Study DNAzyme Structure and FunctionJan Borggrдfe and Manuel Etzkorn 11. Molecular Modeling and Simulations of DNA and RNA: DNAzyme as a Model SystemChristoph G.W. Gertzen and Holger Gohlke Part IV Further Biophysical Methods 12. Single-Molecule Kinetic Studies of Nucleic Acids by Fцrster Resonance Energy TransferMйlodie C.A.S. Hadzic, Roland K.O. Sigel, and Richard Bцrner 13. Chemical Dual End-Labeling of Large RibozymesEsra Ahunbay, Fabio D. Steffen, Susann Zelger-Paulus, and Roland K. O. Sigel 14. Spin Labeling of Long RNAs via Click Reaction and Enzymatic LigationMaria Francesca Vicino, Christine Wuebben, Mark Kerzhner, Michael Famulok, and Olav Schiemann 15. Preparation of Site-Specifically Spin-Labeled RNA by In Vitro Transcription using an Expanded Genetic AlphabetLisa Bornewasser and Stephanie Kath-Schorr 16. PELDOR Measurements on Nitroxide-Labelled OligonucleotidesTobias Hett and Olav Schiemann Part V Use of DNAzymes 17. Sensing Metal Ions with Phosphorothioate-Modified DNAzymesPo-Jung Jimmy Huang and Juewen Liu 18. Enhancement of Activity for Peroxidase-Mimicking DNAzyme by Covalent Attachment of Hemin to G-quadruplex-Forming Oligonucleotide Using Click ChemistryJoanna Kosman 19. Generation of RNA with 2', 3'-cyclic Phosphates by Deoxyribozyme Cleavage in Frozen SolutionsKristian K. Le Vay and Hannes Mutschler Subject Index List...
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