Автор: Patil Nayana, Sivaram Aruna Название: A Complete Guide to Gene Cloning: From Basic to Advanced ISBN: 3030968537 ISBN-13(EAN): 9783030968533 Издательство: Springer Рейтинг: Цена: 11878.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This comprehensive guide to gene cloning provides beginning and advanced readers with the background, standard techniques, practical applications, and ethical and safety considerations in the field. A one-stop reference for students, researchers in academia and industry, and anyone interested in a thorough but accessible overview.
Автор: Hardin Название: Cloning Gene Expression ISBN: 0195132947 ISBN-13(EAN): 9780195132946 Издательство: Oxford Academ Рейтинг: Цена: 11088.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The central purpose of this combination lecture/laboratory manual is to present detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties. The manual includes 20 experiments designed to train students to prepare, manipulate, and analyzse plasmids, to produce fusion proteins in bacteria, and to purify these proteins based on unique chemical properties or substrate affinities. Electrophoresis, southern and western blotting and cominatorial techniques are empsized. Radioisotopes, fluorophores and solvent effects on protein structure are discussed. (No radioisotopes are required.) The experiments included have been selected because of their high success rate and because they emphasize a project-oriented approach. Sufficient detail and background are provided such that the intended audience includes advanced undergraduate students, beginning graduates, and practising professionals engaged in a broad range of pursuits. Each experiment is accompanied by detailed process rationale.
Автор: Schafer, Susan Название: Cloning ISBN: 0765681382 ISBN-13(EAN): 9780765681386 Издательство: Taylor&Francis Рейтинг: Цена: 20671.00 р. Наличие на складе: Поставка под заказ.
Автор: Barbara MacKinnon Название: Human Cloning: Science, Ethics, and Public Policy ISBN: 0252070585 ISBN-13(EAN): 9780252070587 Издательство: Wiley EDC Рейтинг: Цена: 3602.00 р. Наличие на складе: Поставка под заказ.
Описание:
Is human cloning a science fiction nightmare come true? Or is it a practical avenue toward beneficial new medical treatments and techniques?
In this concise volume, experts on all sides of the debate make arguments for why we should either pursue, regulate, or ban the cloning of human beings. From this collection, readers will gain a clearer picture of the history of cloning in agriculture and animal science, the various biological procedures that are encompassed by the term "cloning," the philosophical arguments in support of and opposed to cloning humans, and the considerations that should inform discussions about public policy matters related both the cloning research and to human cloning itself.
Balancing scientific detail with philosophical argument, Human Cloning succinctly outlines what cloning is and is not (e.g., cloning does not produce identical individuals), what has led to recent scientific developments, what is now possible, and what ethical dilemmas cloning presents. Opponents claim that cloning subverts human dignity, makes a mockery of spousal love, and poses serious safety hazards. Proponents cite a range of potential benefits, such as producing transplant tissue that is less likely to be rejected, extending current techniques of artificial insemination, and controlling genetic abnormalities to prevent birth defects.
Cloning itself is not new, but as the science of cloning continues to advance--and as human cloning thus inches closer to reality--we are compelled to consider its implications for society. Human Cloning is a lucid, substantive guide to this contentious and potentially revolutionary issue.
Автор: Louis-Marie Houdebine; Jianglin Fan Название: Rabbit Biotechnology ISBN: 9048184762 ISBN-13(EAN): 9789048184767 Издательство: Springer Рейтинг: Цена: 19589.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: With a specific biotechnology of the rabbit and its use in scientific research emerging, this timely book sets out the sophisticated approaches developed independently in different academic and industrial laboratories as well as in companies around the world.
Автор: Cibelli, Jose Название: Principles Of Cloning ISBN: 0123865417 ISBN-13(EAN): 9780123865410 Издательство: Elsevier Science Рейтинг: Цена: 29139.00 р. Наличие на складе: Поставка под заказ.
Описание: Focuses on the science of cloning. This book presents the basic biological mechanisms of how cloning works and progresses to discuss current and potential applications in basic biology, agriculture, biotechnology, and medicine.
Автор: Haran, Joan Название: Human Cloning in the Media ISBN: 0415759668 ISBN-13(EAN): 9780415759663 Издательство: Taylor&Francis Рейтинг: Цена: 7042.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Автор: Seok-Yong Choi; Hyunju Ro; Hankuil Yi Название: DNA Cloning: A Hands-on Approach ISBN: 9402416609 ISBN-13(EAN): 9789402416602 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.
Автор: Sambrook, Joseph Russell, David Название: Molecular cloning ISBN: 0879695773 ISBN-13(EAN): 9780879695774 Издательство: NBN International Рейтинг: Цена: 37805.00 р. Наличие на складе: Нет в наличии.
Описание: In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology.
The natural world is marked by an ever-increasing loss of varied habitats, a growing number of species extinctions, and a full range of new kinds of dilemmas posed by global warming. At the same time, humans are also working to actively shape this natural world through contemporary bioscience and biotechnology. In Cloning Wild Life, Carrie Friese posits that cloned endangered animals in zoos sit at the apex of these two trends, as humans seek a scientific solution to environmental crisis. Often fraught with controversy, cloning technologies, Friese argues, significantly affect our conceptualizations of and engagements with wildlife and nature. By studying animals at different locations, Friese explores the human practices surrounding the cloning of endangered animals. She visits zoos—the San Diego Zoological Park, the Audubon Center in New Orleans, and the Zoological Society of London—to see cloning and related practices in action, as well as attending academic and medical conferences and interviewing scientists, conservationists, and zookeepers involved in cloning. Ultimately, she concludes that the act of recalibrating nature through science is what most disturbs us about cloning animals in captivity, revealing that debates over cloning become, in the end, a site of political struggle between different human groups. Moreover, Friese explores the implications of the social role that animals at the zoo play in the first place—how they are viewed, consumed, and used by humans for our own needs. A unique study uniting sociology and the study of science and technology, Cloning Wild Life demonstrates just how much bioscience reproduces and changes our ideas about the meaning of life itself.
Автор: Friese Carrie Название: Cloning Wild Life ISBN: 0814729088 ISBN-13(EAN): 9780814729083 Издательство: Wiley EDC Рейтинг: Цена: 12012.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
The natural world is marked by an ever-increasing loss of varied habitats, a growing number of species extinctions, and a full range of new kinds of dilemmas posed by global warming. At the same time, humans are also working to actively shape this natural world through contemporary bioscience and biotechnology. In Cloning Wild Life, Carrie Friese posits that cloned endangered animals in zoos sit at the apex of these two trends, as humans seek a scientific solution to environmental crisis. Often fraught with controversy, cloning technologies, Friese argues, significantly affect our conceptualizations of and engagements with wildlife and nature. By studying animals at different locations, Friese explores the human practices surrounding the cloning of endangered animals. She visits zoos—the San Diego Zoological Park, the Audubon Center in New Orleans, and the Zoological Society of London—to see cloning and related practices in action, as well as attending academic and medical conferences and interviewing scientists, conservationists, and zookeepers involved in cloning. Ultimately, she concludes that the act of recalibrating nature through science is what most disturbs us about cloning animals in captivity, revealing that debates over cloning become, in the end, a site of political struggle between different human groups. Moreover, Friese explores the implications of the social role that animals at the zoo play in the first place—how they are viewed, consumed, and used by humans for our own needs. A unique study uniting sociology and the study of science and technology, Cloning Wild Life demonstrates just how much bioscience reproduces and changes our ideas about the meaning of life itself.
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