Microbial Metagenomics, Metatranscriptomics, And Metaproteomics,531, DeLong,Ed
Автор: Wolfgang Streit; Rolf Daniel Название: Metagenomics ISBN: 1493966898 ISBN-13(EAN): 9781493966899 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. Construction of Small-Insert and Large-Insert Metagenomic Libraries Carola Simon and Rolf Daniel 2. Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies Dominik Schneider, Franziska Wemheuer, Birgit Pfeiffer, and Bernd Wemheuer 3. Construction and Screening Marine Metagenomic Large Insert Libraries Nancy Weiland-Brдuer, Daniela Langfeldt, and Ruth A. Schmitz 4. Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment Mikkel A. Glaring, Jan K. Vester, and Peter Stougaard 5. DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms Eleanor Jameson, Martin Taubert, Sara Coyotzi, Yin Chen, Цzge Eyice, Hendrik Schдfer, J. Colin Murrell, Josh D. Neufeld, and Marc G. Dumont 6. Assessing Bacterial and Fungal Diversity in the Plant's Endosphere Bernd Wemheuer and Franziska Wemheuer 7. Shotgun Metagenomic Sequencing analysis of Soft-Rot Enterobacteriaceae in Polymicrobial Communities James Doonan, Sandra Denman, James E. McDonald, and Peter M. Golyshin 8. Cloning and Expression of Metagenomic DNA in Streptomyces lividans and its Subsequent Fermentation for Optimized Production Yuriy Rebets, Jan Kormanec, Andriy Lutzhetskyy, Kristel Bernaerts, and Jozef Annй 9. Degradation Network Reconstruction Guided by Metagenomic Data Rafael Bargiela and Manuel Ferrer 10. Novel Tools for the Functional Expression of Metagenomics DNA Nadine Katzke, Andreas Knapp, Anita Loeschcke, Thomas Drepper, and Karl-Erick Jaeger 11. A Microtiter Plate-Based Assay to Screen for Active and Stereoselective Hydrolytic Enzymes in Enzyme Libraries Dominique Bцttcher, Patrick Zдgel, Marlen Schmidt, and Uwe T. Bornscheuer 12. Screening for Cellulase Encoding Clones in Metagenomics Libraries Nele Ilmberger and Wolfgang R. Streit 13. Liquid Phase Multiplex High-Throughput Screening of Metagenomic Libraries using p-Nitrophenyl-Linked Substrates for Accessory Lignocellulosic Enzymes Mariette Smart, Robert J. Huddy, Don A. Cowan, and Marla Trindade 14. Screening Glycosyltransferases for Polyphenol Modifications Nele Ilmberger and Ulrich Rabausch 15. Methods for the Isolation of Genes Encoding Novel PHA Matabolism Enzymes from Complex Microbial CommunitiesJiujun Cheng, Ricardo Nordeste, Maria A. Trainer, and Trevor C. Charles 16. Function-Based Metagenomic Library Screening and Heterologous Expression Strategy for Genes Encoding Phosphatase Activity Genis A. Castillo Villamizar, Heiko Nacke, and Rolf Daniel 17. Activity-Based Screening of Metagenomics Libraries for Hydrogenase Enzymes Nicole Adam and Mirjam Perner 18. Screening for N-AHSL-Based-Signaling Interfering Enzymes Stйphane Uroz and Phil M. Oger 19. Mining Microbial Signals for Enhanced Biodiscovery of Secondary Metabolites F. Jerry Reen, Jose A. Gutiйrrez-Barranquero, and Fergal O'Gara
Автор: Vipin Chandra Kalia; Yogesh Shouche; Hemant J. Pur Название: Mining of Microbial Wealth and MetaGenomics ISBN: 9811354685 ISBN-13(EAN): 9789811354687 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Поставка под заказ.
Описание: The existence of living organisms in diverse ecosystems has been the focus of interest to human beings, primarily to obtain insights into the diversity and dynamics of the communities.
Автор: Wolfgang R. Streit; Rolf Daniel Название: Metagenomics ISBN: 1493961233 ISBN-13(EAN): 9781493961238 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Metagenomics has proven to be a powerful tool for exploring the ecology, metabolic profiling, and comparison of complex microbial communities as well as its important applications in the mining of metagenomes for genes encoding novel biocatalysts and drug molecules for bioindustries. In Metagenomics: Methods and Protocols, expert researches provide an overview and introduction to basic methods commonly used in laboratories that have a strong background in microbial metagenomics. The book attempts to address all of the working steps involved in this crucial field, beginning with DNA isolation from soils and marine samples and continuing with the construction and screening of libraries, along with key advise involving bioinformatic tools available to analyze large metagenomic sequence data sets. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Metagenomics: Methods and Protocols serves as a very complete guide to available screening protocols for all major biocatalysts in order to allow for the easy setup of these screens in any microbiology lab.
Описание: This book summarizes the various areas of research in metagenomics and their potential applications in medicine, the environment and biotechnology.
Автор: Streit Wolfgang R., Daniel Rolf Название: Metagenomics: Methods and Protocols ISBN: 1493982745 ISBN-13(EAN): 9781493982745 Издательство: Springer Рейтинг: Цена: 12577.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This second edition explores up-to-date tools in various function-based technologies currently used in metagenomics. The chapters in this book discuss all of the working steps involved in these technologies, such as: DNA isolation from soils and marine samples followed by the construction and screening of libraries for diverse enzymes and biomolecules; current methods used to isolate DNA and construct large- and small-insert libraries from terrestrial and marine habitats; methods for establishing metagenome libraries in none-E.coli hosts; new molecular tools used for function-driven mining of metagenomic DNA; and screening protocols for a wide array of different genes encoding enzymes with relevance to biotechnology and ecology. Metagenomics: Methods and Protocols, Second Edition also provides detailed screening protocols for phosphatases, poly-hydroxyalkanoate, metabolism-related enzymes, stereoselective hydrolases, and microbial signals for the discovery of secondary metabolites.
Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Metagenomics: Methods and Protocols, Second Edition is a collection of up-to-date metagenome protocols and tools for the recovery of numerous major types of biocatalysts, and allows researchers to easily setup these screens in any microbiology laboratory.
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