The Oxford Introduction to Proto-Indo-European and the Proto-Indo-European World, Mallory, J. P.; Adams, D. Q.

Автор: Armstrong Donald
Название: Oxidative Stress Biomarkers and Antioxidant Protocols
ISBN: 0896038505 ISBN-13(EAN): 9780896038509
Издательство: Springer
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Цена: 21661.00 р.
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Описание: Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the pertubations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltametry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols (0-89603-851-3), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays.

Автор: Proetzel
Название: Antibody Methods and Protocols
ISBN: 1617799300 ISBN-13(EAN): 9781617799303
Издательство: Springer
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Цена: 20962.00 р.
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Описание: The rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies.  Antibody Methods and Protocols attempts to provide insight into the generation of antibodies using in vitro and in vivo approaches, as well as technical aspects for screening, analysis, and modification of antibodies and antibody fragments.  The detailed volume is focused on basic protocols for isolating antibodies and, at the same time, it selects a range of specific areas with the aim of providing guides for the overall process of antibody isolation and characterization as well as protocols for enhancing classical antibodies and antibody fragments.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Antibody Methods and Protocols provides a broad and useful background to support ongoing efforts by novices and experts alike and encourages the development of new imaginative approaches to this vital area of study.

Автор: Connell Joe
Название: RT-PCR Protocols
ISBN: 0896038750 ISBN-13(EAN): 9780896038752
Издательство: Springer
Цена: 13270.00 р.
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Описание: A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.

Автор: Proto
Название: Baudrillard For Architects Proto
ISBN: 0415508851 ISBN-13(EAN): 9780415508858
Издательство: Taylor&Francis
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Цена: 13779.00 р.
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Описание: This introductory but in-depth assessment and critique of Baudrillard`s interest in architecture presents key concepts and emphasises how the mutual concerns of cultural theory and architecture provide a fruitful ground for debate in contemporary architecture.

Автор: Lo Benny K. C.
Название: Antibody Engineering / Methods and Protocols
ISBN: 1588290921 ISBN-13(EAN): 9781588290922
Издательство: Springer
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Цена: 24456.00 р.
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Описание: A core collection of diverse cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Procedures are also described on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a wide variety of in vivo applications. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics.

Автор: John T. Queenan,Catherine Y. Spong,Charles J. Lock
Название: Protocols for High–Risk Pregnancies: An Evidence–Based Approach
ISBN: 1119000874 ISBN-13(EAN): 9781119000877
Издательство: Wiley
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Цена: 13187.00 р.
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Описание: High–risk pregnancies present life–threatening challenges to two of your patients: the mother and her fetus. The direct, exemplary guidance in Protocols for High–Risk Pregnancy enables you to better understand your patients? conditions devise optimum management strategies maximize the outcome and minimize the complications for both the mother and her fetus To enhance clinical relevance, each protocol is written as if the patient were present. Evidence to support an intervention is given where it exists. The authors? experience provides additional wise counsel. Key references provide the springboard for a deeper understanding of a topic. In this more compact and fully updated sixth edition, new protocols include Amniotic fluid disorders Depression Fetal growth restriction HIV Indicated late preterm and early term birth Malaria Noninvasive prenatal diagnosis Designed for clinical practice by the leaders of two generations of maternal–fetal medicine, no obstetrician or obstetric health care provider can afford to miss Protocols for High–Risk Pregnancy.inciples and practice of this important topic.clinicians caring for patients with congenital heart disease.This practical, spiral–bound, go–to reference is designed to answer the most frequently–posed questions about bypass for CHD, the surgical treatment of which is often complicated, with some conditions seen only rarely in  practice. Perfusion for Congenital Heart Surgery is written and designed for regular use:–  Focuses on the latest devices and techniques and provides clear, matter–of–fact guidance on their roles and importance to CHD procedures–  Written by a seasoned perfusionist who is co–chief and educator at one of the world s leading children s hospitals–  The spiral binding allows the book to be laid flat for easy referral–  A Notes section, following the discussion of each CHD lesion, enables readers to add institution and/or surgeon–specific information for later referenceinteractive text is also an excellent supplemental handbook for students, junior medical staff and medical practitioners.        A companion website to this title is available at www.rchhandbook.org This new edition of the best–selling manual, The Royal Children s Hospital Melbourne Paediatric Handbook 9th Edition is a succinct and practical guide to managing common and serious disorders in childhood. Relevant to medical, nursing and allied health fields, this is both a useful source for revision and an essential guide to clinical management and rapid decision–making at the point of care. For this edition, the handbook has been restructured according to body systems and is now more closely aligned with the Royal Children s Hospital Clinical Practice Guidelines.  It also includes updated evidence–based information in all chapters, an additional genetics chapter, new renal and oncology chapters and fully updated drug doses. The Handbook includes: Clear illustrations, and plenty of accessible diagnostic and management algorithms A Paediatric Pharmacopoeia Step–by–step guidelines for paediatric procedures Emphasis on the modern, community–based approach to the management of children s problems A strong emphasis on practical management by the doctor at first contact With well–presented, summarized information that you can easily access during a consultation, this text is also an excellent supplemental handbook for students, junior medical staff and medical practitioners.