Yeast Protocols, Wei Xiao

Автор: Farshad Darvishi Harzevili
Название: Biotechnological Applications of the Yeast Yarrowia lipolytica
ISBN: 3319064363 ISBN-13(EAN): 9783319064369
Издательство: Springer
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Описание: Due to its ability to degrade hydrophobic substrates highly efficiently, the non-conventional yeast Yarrowia lipolytica offers various applications in the food, feed, pharmaceutical, and fine chemistry industries, as well as in environmental protection contexts such as waste treatment and bioremediation. The yeast breaks down hydrophobic substrates such as n-alkanes, fatty acids, fats, and oils, yielding valuable biotechnological products such as organic acids, extracellular enzymes, aroma compounds, bioemulsifiers, polyols, single-cell proteins, and single-cell oils. Further, Y. lipolytica is an efficient platform for the production of various heterologous proteins; more than a hundred heterologous proteins – including those of viruses, bacteria and fungi, as well as plant, animal and human proteins – have already been produced in Y. lipolytica. Since its genome has been sequenced, it is now possible to use new recombinant technology and metabolic engineering in order to improve the metabolic pathways involved in the creation of desirable metabolites and products.

Автор: Tulasi Satyanarayana; Gotthard Kunze
Название: Yeast Diversity in Human Welfare
ISBN: 9811026203 ISBN-13(EAN): 9789811026201
Издательство: Springer
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Описание: This book brings together and updates the latest information on the diversity of yeasts, their molecular features and their applications in the welfare of mankind.

Автор: Graham G. Stewart
Название: Distilling and Brewing Yeast
ISBN: 3319691244 ISBN-13(EAN): 9783319691244
Издательство: Springer
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Цена: 30745.00 р.
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Описание: Yeast stress, vitality and viability are also addressed together with flavor production, genetic manipulation, bioethanol formation and ethanol production by non-Saccharomyces yeasts and a Gram-negative bacterium.

Автор: Alistair J.P. Brown; Mick F. Tuite; John E.G. McCa
Название: Protein Synthesis and Targeting in Yeast
ISBN: 3642849237 ISBN-13(EAN): 9783642849237
Издательство: Springer
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Цена: 16979.00 р.
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Описание: Proceedings of the NATO Advanced Research Workshop on Protein Synthesis and Targeting in Yeast, held at Canterbury, Kent, UK, July 4-8, 1992

Автор: Bin Liu
Название: Yeast Surface Display
ISBN: 1493927477 ISBN-13(EAN): 9781493927470
Издательство: Springer
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Цена: 19564.00 р.
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Описание: Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering, yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications.

Автор: Wei Xiao
Название: Yeast Protocols
ISBN: 1493942778 ISBN-13(EAN): 9781493942770
Издательство: Springer
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Цена: 15372.00 р.
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Описание:

1. Basic Investigations in Saccharomyces cerevisiae

Brendan Curran and Virginia Bugeja

2. Isolation of Yeast Nucleic Acids

Michael Biss, Michelle Hanna and Wei Xiao

3. Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry

Ting Wei and Heping Dai

4. Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method

R. Daniel Gietz

5. Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe

Johannes H. Hegemann, Sven Boris Heick, Jennifer Pцhlmann, Markus Langen and Ursula Fleig

6. See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry

Changhui Deng and Andrew N. Krutchinsky

7. Yeast Two Hybrid Liquid Screening

Elisa Donnard, Erica M. Queiroz, J. Miguel Ortega, and R. Daniel Gietz

8. Targeted Mutagenesis of a Specific Gene in Yeast

Likui Zhang, Alina Radziwon, and Linda J. Reha-Krantz

9. Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae

Kai Stefan Dimmer

10. Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy

Wei-Pang Huang, Takahiro Shintani, and Zhiping Xie

11. Assays for Autophagy II: Mitochondrial Autophagy

Tomotake Kanki and Koji Okamoto

12. Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction

Danielle Hagstrom and Changle Ma

13. Yeast Survival and Growth Assays

Xin Xu, Amanda Lambrecht, and Wei Xiao

14. Spontaneous Mutagenesis Assay

Susan Blackwell, Michelle Hanna, and Wei Xiao

15. Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene

Bernard A. Kunz

16. piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe

Jun Li and Li-Lin Du

17. Replicative and Chronological Life Span Assays

Spike D. L. Postnikoff and Troy A. A. Harkness

18. Detection of Protein Arginine Methylation in Saccharomyces cerevisiae

Christopher Aa. Jackson and Michael C. Yu

19. Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae

Lindsay G. Ball and Wei Xiao

20. Ligand Engineering Using Yeast Surface Display

Benjamin J. Hackel

21. Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay

Kris Barreto and C. Ronald Geyer

Автор: Michael Breitenbach; S. Michal Jazwinski; Peter La
Название: Aging Research in Yeast
ISBN: 9400794207 ISBN-13(EAN): 9789400794207
Издательство: Springer
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Цена: 26120.00 р.
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Описание: Collecting current research on yeast aging, this book covers mitochondrial theories of aging, emphasizing oxidative stress and retrograde responses; autophagy and mitophagy; the relationship of apoptosis to aging processes; replication stress and more.

Автор: Jure Pi?kur; Concetta Compagno
Название: Molecular Mechanisms in Yeast Carbon Metabolism
ISBN: 3642550126 ISBN-13(EAN): 9783642550126
Издательство: Springer
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Описание: Yeast is one of the most studied laboratory organisms and represents one of the most central models to understand how any eukaryote cell works. On the other hand, yeast fermentations have for millennia provided us with a variety of biotech products, like wine, beer, vitamins, and recently also with pharmaceutically active heterologous products and biofuels. A central biochemical activity in the yeast cell is the metabolism of carbon compounds, providing energy for the whole cell, and precursors for any of the final fermentation products. A complex set of genes and regulatory pathways controls the metabolism of carbon compounds, from nutrient sensing, signal transduction, transcription regulation and post-transcriptional events. Recent advances in comparative genomics and development of post-genomic tools have provided further insights into the network of genes and enzymes, and molecular mechanisms which are responsible for a balanced metabolism of carbon compounds in the yeast cell, and which could be manipulated in the laboratory to increase the yield and quality of yeast biotech products. This book provides a dozen of most comprehensive reviews on the recent developments and achievements in the field of yeast carbon metabolism, from academic studies on gene expression to biotechnology relevant topics.

Автор: Juan I. Castrillo; Stephen G. Oliver
Название: Yeast Systems Biology
ISBN: 1493961357 ISBN-13(EAN): 9781493961351
Издательство: Springer
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Цена: 23058.00 р.
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Описание: This book presents an up-to-date view of yeast as a model eukaryote for systems biology studies. It provides practical, easy to use methodologies and includes key tips and vital implementation advice from the experts.