Автор: Nicola King Название: RT-PCR Protocols ISBN: 1607616289 ISBN-13(EAN): 9781607616283 Издательство: Springer Рейтинг: Цена: 23757.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book fully updates the RT-PCR technologies presented in the popular first edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells and RT-PCR for cloning. In addition, this edition explores new technologies.
Автор: Rajyalakshmi Luthra; Rajesh Singh; Keyur Pravincha Название: Clinical Applications of PCR ISBN: 1493933582 ISBN-13(EAN): 9781493933587 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled with mass spectroscopy, and high throughput NGS techniques.
Автор: Bimal D.M. Theophilus; Ralph Rapley Название: PCR Mutation Detection Protocols ISBN: 1493956299 ISBN-13(EAN): 9781493956296 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR). In PCR Mutation Detection Protocols, Second Edition, expert researchers bring the subject up-to-date with key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The volume also addresses key areas such as Southern blotting, accurate diagnostics with high throughput, as well as microarray systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which provide the often hard to find information that may mean the difference between the success and failure of the method. Authoritative and cutting-edge, PCR Mutation Detection Protocols, Second Edition aims to stimulate postgraduate scientists, researchers, and clinicians already engaged in the area and to provide an important first step for those new to this practice wanting to adopt the powerful and essential technique in their own laboratories.
Автор: Bing-Yuan Chen; Harry W. Janes Название: PCR Cloning Protocols ISBN: 1617372811 ISBN-13(EAN): 9781617372810 Издательство: Springer Рейтинг: Цена: 25853.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: PCR Cloning Protocols, Second Edition, updates and expands Bruce White`s best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis.
Автор: Nicola King Название: RT-PCR Protocols ISBN: 1493956825 ISBN-13(EAN): 9781493956821 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.
Автор: Anton Yuryev Название: PCR Primer Design ISBN: 1617377430 ISBN-13(EAN): 9781617377433 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.
Автор: Najafov Ayaz Название: PCR Guru ISBN: 0128042311 ISBN-13(EAN): 9780128042311 Издательство: Elsevier Science Рейтинг: Цена: 9936.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is written with an aim of providing researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. PCR Guru covers the history of PCR and the basic science underlying it and discusses what PCR is on the bench level starting with detailed description and tips on primer design and continuing with what are the standard protocols used to perform PCR.
Provides troubleshooting tips for various types of modifications of standard protocols
Contains unique "Good Practices and Tips┐ that are indispensable for the beginner and expert alike
Features "Special Cases┐ with applications of PCR, optimization, and troubleshooting
Includes detailed appendices with tables, figures, and key protocols
Organized as a systematic, concentrated resource to save time when addressing a PCR problem
Автор: Mark Wilks Название: PCR Detection of Microbial Pathogens ISBN: 1493960997 ISBN-13(EAN): 9781493960996 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one`s own tests or introducing tests developed by other workers.
Автор: U. Reischl; C. Wittwer; F. Cockerill Название: Rapid Cycle Real-Time PCR — Methods and Applications ISBN: 3642483534 ISBN-13(EAN): 9783642483530 Издательство: Springer Рейтинг: Цена: 15672.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences.
Автор: Roberto Biassoni; Alessandro Raso Название: Quantitative Real-Time PCR ISBN: 149394665X ISBN-13(EAN): 9781493946655 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ? Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation. --
Автор: Y. M. Dennis Lo Название: Clinical Applications of PCR ISBN: 0896033597 ISBN-13(EAN): 9780896033597 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal molecular medicine. Section 3 includes carefully chosen examples that represent typical applications of PCR in diverse clinical fields, encompassing hematology, oncology, genetics, and microbiology.
Автор: Gerrit J. Viljoen; Louis H. Nel; John R. Crowther Название: Molecular Diagnostic PCR Handbook ISBN: 9048168562 ISBN-13(EAN): 9789048168569 Издательство: Springer Рейтинг: Цена: 28732.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
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