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Quantitative Real-Time PCR, Roberto Biassoni; Alessandro Raso


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Автор: Roberto Biassoni; Alessandro Raso
Название:  Quantitative Real-Time PCR
ISBN: 9781493946655
Издательство: Springer
Классификация:


ISBN-10: 149394665X
Обложка/Формат: Paperback
Страницы: 231
Вес: 0.43 кг.
Дата издания: 23.08.2016
Серия: Methods in Molecular Biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 18 tables, black and white; 66 illustrations, color; 14 illustrations, black and white; xi, 231 p. 80 illus., 66 illus. in color.
Размер: 254 x 178 x 13
Читательская аудитория: Professional & vocational
Основная тема: Life Sciences
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ? Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation. --
Дополнительное описание: Twenty Years of qPCR: A Mature Technology?.- Minimum Information Necessary for Quantitative Real-Time PCR Experiments.- Selection of Reliable Reference Genes for RT-qPCR Analysis.- Introduction to Digital PCR.- mRNA and micro RNA Purity and Integrity: The



PCR Protocols

Автор: Daniel J. Park
Название: PCR Protocols
ISBN: 1627038191 ISBN-13(EAN): 9781627038195
Издательство: Springer
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Цена: 20263.00 р.
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Описание: In its 3rd Edition, this book selects recently developed tools and tricks, for the value they add to established methods. The text describes core applications: PCR cloning and sequencing, expression, copy number or methylation profile analysis, and more.

PCR Mutation Detection Protocols

Автор: Bimal D.M. Theophilus; Ralph Rapley
Название: PCR Mutation Detection Protocols
ISBN: 1493956299 ISBN-13(EAN): 9781493956296
Издательство: Springer
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Цена: 19564.00 р.
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Описание: Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR). In PCR Mutation Detection Protocols, Second Edition, expert researchers bring the subject up-to-date with key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The volume also addresses key areas such as Southern blotting, accurate diagnostics with high throughput, as well as microarray systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which provide the often hard to find information that may mean the difference between the success and failure of the method. Authoritative and cutting-edge, PCR Mutation Detection Protocols, Second Edition aims to stimulate postgraduate scientists, researchers, and clinicians already engaged in the area and to provide an important first step for those new to this practice wanting to adopt the powerful and essential technique in their own laboratories.

PCR Detection of Microbial Pathogens

Автор: Mark Wilks
Название: PCR Detection of Microbial Pathogens
ISBN: 1493960997 ISBN-13(EAN): 9781493960996
Издательство: Springer
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Цена: 16769.00 р.
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Описание: PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one`s own tests or introducing tests developed by other workers.

PCR Primer Design

Автор: Anton Yuryev
Название: PCR Primer Design
ISBN: 1617377430 ISBN-13(EAN): 9781617377433
Издательство: Springer
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Цена: 18167.00 р.
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Описание: At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.

RT-PCR Protocols

Автор: Nicola King
Название: RT-PCR Protocols
ISBN: 1493956825 ISBN-13(EAN): 9781493956821
Издательство: Springer
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Цена: 20962.00 р.
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Описание: Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.

PCR Primer Design

Автор: Yuryev Anton
Название: PCR Primer Design
ISBN: 158829725X ISBN-13(EAN): 9781588297259
Издательство: Springer
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Цена: 23757.00 р.
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Описание: At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.

RT-PCR Protocols

Автор: Nicola King
Название: RT-PCR Protocols
ISBN: 1607616289 ISBN-13(EAN): 9781607616283
Издательство: Springer
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Цена: 23757.00 р.
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Описание: This book fully updates the RT-PCR technologies presented in the popular first edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells and RT-PCR for cloning. In addition, this edition explores new technologies.

Molecular and Quantitative Animal Genetics. HasanKhatib (ed).2015.-Wiley-Backwell; 1 edition (March 2, 2015)

Название: Molecular and Quantitative Animal Genetics. HasanKhatib (ed).2015.-Wiley-Backwell; 1 edition (March 2, 2015)
ISBN: 1118677404 ISBN-13(EAN): 9781118677407
Издательство: Wiley
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Цена: 14090.00 р.
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Описание: Animal genetics is a foundational discipline in the fields of animal science, animal breeding, and veterinary sciences. While genetics underpins the healthy development and breeding of all living organisms, this is especially true in domestic animals, specifically with respect to breeding for key traits.

Quantitative Trait Loci (QTL)

Автор: Scott A. Rifkin
Название: Quantitative Trait Loci (QTL)
ISBN: 1493958321 ISBN-13(EAN): 9781493958320
Издательство: Springer
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Цена: 20263.00 р.
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Описание: This Methods in Molecular Biology (TM) volume offers techniques for the entire process of quantitative train loci experiments: the mapping populations, identifying quantitative trait loci, extending the power of quantitative trait locus analysis and case studies.

Analytical and Quantitative Cardiology

Автор: S. Sideman; Rafael Beyar
Название: Analytical and Quantitative Cardiology
ISBN: 1461377315 ISBN-13(EAN): 9781461377313
Издательство: Springer
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Цена: 6986.00 р.
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Описание: Proceedings of the 10th Goldberg Workshop held in Haifa, Israel, December 2-5, 1996

Likelihood, Bayesian, and MCMC Methods in Quantitative Genetics

Автор: Daniel Sorensen; Daniel Gianola
Название: Likelihood, Bayesian, and MCMC Methods in Quantitative Genetics
ISBN: 1441929975 ISBN-13(EAN): 9781441929976
Издательство: Springer
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Цена: 26120.00 р.
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Описание: This book, suitable for numerate biologists and for applied statisticians, provides the foundations of likelihood, Bayesian and MCMC methods in the context of genetic analysis of quantitative traits.

Statistical Genetics of Quantitative Traits

Автор: Rongling Wu; Changxing Ma; George Casella
Название: Statistical Genetics of Quantitative Traits
ISBN: 1441919120 ISBN-13(EAN): 9781441919120
Издательство: Springer
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Цена: 22201.00 р.
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Описание: Aimed at graduate students and researchers and including statistical models and analytical procedures, this book introduces the basic concepts and methods used in the statistical analysis and modeling of the DNA-based marker and phenotypic data that arise in agriculture and related fields.


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