PCR Primer Design, Anton Yuryev

Автор: Connell Joe
Название: RT-PCR Protocols
ISBN: 0896038750 ISBN-13(EAN): 9780896038752
Издательство: Springer
Цена: 13270.00 р.
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Описание: A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.

Автор: Mark Wilks
Название: PCR Detection of Microbial Pathogens
ISBN: 1493960997 ISBN-13(EAN): 9781493960996
Издательство: Springer
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Цена: 16769.00 р.
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Описание: PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one`s own tests or introducing tests developed by other workers.

Автор: Yuryev Anton
Название: PCR Primer Design
ISBN: 158829725X ISBN-13(EAN): 9781588297259
Издательство: Springer
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Цена: 23757.00 р.
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Описание: At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.

Автор: Bimal D.M. Theophilus; Ralph Rapley
Название: PCR Mutation Detection Protocols
ISBN: 1493956299 ISBN-13(EAN): 9781493956296
Издательство: Springer
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Цена: 19564.00 р.
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Описание: Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR). In PCR Mutation Detection Protocols, Second Edition, expert researchers bring the subject up-to-date with key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The volume also addresses key areas such as Southern blotting, accurate diagnostics with high throughput, as well as microarray systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which provide the often hard to find information that may mean the difference between the success and failure of the method. Authoritative and cutting-edge, PCR Mutation Detection Protocols, Second Edition aims to stimulate postgraduate scientists, researchers, and clinicians already engaged in the area and to provide an important first step for those new to this practice wanting to adopt the powerful and essential technique in their own laboratories.

Автор: Sergio Colonna-Romano; Antonella Leone; Bruno Mare
Название: Differential-Display Reverse Transcription-PCR (DDRT-PCR)
ISBN: 3540632972 ISBN-13(EAN): 9783540632979
Издательство: Springer
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Цена: 18167.00 р.
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Описание: Identification of differentially expressed genes is one of the major challenges in molecular biology. This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types.

Автор: Roberto Biassoni; Alessandro Raso
Название: Quantitative Real-Time PCR
ISBN: 149394665X ISBN-13(EAN): 9781493946655
Издательство: Springer
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Цена: 13974.00 р.
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Описание: Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ? Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation. --

Автор: Daniel J. Park
Название: PCR Protocols
ISBN: 1627038191 ISBN-13(EAN): 9781627038195
Издательство: Springer
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Цена: 20263.00 р.
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Описание: In its 3rd Edition, this book selects recently developed tools and tricks, for the value they add to established methods. The text describes core applications: PCR cloning and sequencing, expression, copy number or methylation profile analysis, and more.

Автор: Stephen J Meltzer
Название: PCR in Bioanalysis
ISBN: 1617370525 ISBN-13(EAN): 9781617370526
Издательство: Springer
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Цена: 20263.00 р.
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Описание: Examines the evolution of US federal education policy and outlines a bold and controversial vision for its future. Jack Jennings offers a vivid analysis of federal efforts in the education arena and reveals some of the factors that shaped their enactment. His rich descriptions and lively anecdotes provide pointed lessons about the partisan climate that stymies much federal policy making today.

Автор: Franck Pellestor
Название: PRINS and In Situ PCR Protocols
ISBN: 1617376361 ISBN-13(EAN): 9781617376368
Издательство: Springer
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Цена: 16769.00 р.
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Описание: Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS detection of unique sequences in situ.

Автор: Jiang Gu
Название: In Situ PCR and Related Technology
ISBN: 1468468456 ISBN-13(EAN): 9781468468458
Издательство: Springer
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Цена: 6986.00 р.
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Описание: Ever since the introduction of the polymerase chain reaction (peR) in 1986, morphologists, whose interests lie in the analysis of intact tissue structures, have been attempting to adapt this technique to intact cells or tissue sections to detect low copy numbers of DNA or RNA in situ while preserving tissue morphology.