Transgenic Mouse Methods and Protocols, Marten H. Hofker; Jan van Deursen
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Kwok Pui-Yan Название: Single Nucleotide Polymorphisms / Methods and Protocols ISBN: 0896039684 ISBN-13(EAN): 9780896039681 Издательство: Springer Цена: 5809.00 р. Наличие на складе: Есть (2 шт.) Описание: Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
Описание: Continued refinement of wide-spread access to transgenic technology has allowed for new animal models have been developed that exhibit features of autoimmune disease have been developed that exhibit features of autoimmune disease.
Автор: Allan V. Kalueff; Carisa L. Bergner Название: Transgenic and Mutant Tools to Model Brain Disorders ISBN: 1493961454 ISBN-13(EAN): 9781493961450 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: As the presence of genetically modified animal models in research laboratories has multiplied, the role of genetic factors in the pathogenesis of brain disorders has become particularly important. The refinement of molecular genetic methods has continued to broaden our understanding of the genetic factors associated with a variety of disorders. In "Transgenic and Mutant Tools to Model Brain Disorders", leading scientists specializing in this field contribute a timely collection of recent advances featuring a vast array of topics in order to contribute to the diverse approaches taken toward the evaluation of genetically modified models in biomedical research. Opening with several chapters covering general aspects of genetically modified animal models, the book then continues with detailed chapters on models of specific human brain disorders, including OCD, Rett Syndrome, anxiety disorders, depression, and schizophrenia. As a volume in the successful Neuromethods™ series, the chapters provide authoritative reviews covering the most commonly used approaches in the field. Cutting-edge and concise, "Transgenic and Mutant Tools to Model Brain Disorders" offers a comprehensive and descriptive overview on a variety of topics in neuroscience and biological psychiatry.
Автор: Igor K. Egorov; Chella S. David Название: Transgenic Mice and Mutants in MHC Research ISBN: 3642754449 ISBN-13(EAN): 9783642754449 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume contains papers presented at the international conference on "Transgenic Mice and Mutants in MHC Research," held in Bar Harbor, Maine, in June, 1989. The meeting brought together eighty researchers working in the field. While mouse H-2 mutants have been known for many years their studies continue to contribute a great deal to our understanding of structure/function relationships and evolution of MHC molecules. Recently a new direction of research has emerged on regulation, expression and function of MHC genes using transgenic animals carrying exogenous MHC genes from the same or other species or engineered MHC genes. With the introduction of transgenic mice more specific questions about the various functions of MHC genes can be answered as, for example, the role of soluble and membrane-bound MHC molecules in self tolerance and elimination of specific T cell clones, recognition of human MHC antigens by the mouse immune system, the role of individual human MHC genes in disease susceptibility. New approaches for evaluation of the role of MHC linked and unlinked genes in susceptibility of mice to malignant tumors and their metastases have been also reported. Our purpose has been to provide a forum for discussion of these new developments. Many questions remain to be answered but the necessary tools have become available. We thank the contributors and hope that the readers will benefit from this Pro- ceedings. We wish to acknowledge the financial sponsorship for the meeting by The Pew Charitable Trust and Howard Hughes Medical Institute.
Описание: This Methods in Molecular Biology book details approaches for the detection of DNA and RNA including recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, step-by-step, reproducible protocols, materials lists, tips and more.
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