Yeast Stress Responses, Stefan Hohmann; Willem H. Mager

Автор: Xiao Wei
Название: Yeast protocols
ISBN: 1588294374 ISBN-13(EAN): 9781588294371
Издательство: Springer
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Описание: In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components-including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.

Автор: Alistair J.P. Brown; Mick F. Tuite; John E.G. McCa
Название: Protein Synthesis and Targeting in Yeast
ISBN: 3642849237 ISBN-13(EAN): 9783642849237
Издательство: Springer
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Описание: Proceedings of the NATO Advanced Research Workshop on Protein Synthesis and Targeting in Yeast, held at Canterbury, Kent, UK, July 4-8, 1992

Автор: John F.T. Spencer; Dorothy M. Spencer
Название: Yeasts in Natural and Artificial Habitats
ISBN: 3642081606 ISBN-13(EAN): 9783642081606
Издательство: Springer
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Описание: A Guide to the World of the Yeasts J. F. T. Spencer and D. M. Spencert As the well-known authority on yeasts, the late Professor Rose, frequently pointed out, it is impossible for one person to present, in a single volume, the details of the life, composiotion, habitats, relationships, and actual and potential uses to man- kind of the 500 (at last count) known species of yeasts. This book confirms the truth of this statement. However, our aim is actually more modest than that, and this book is an attempt to introduce the general reader, and possibly some inter- ested specialists, to the lives of the yeasts in their natural and more artificial habitats, their use by human beings, and to give some idea of the wonderfully complex activities within the yeast cell, the characteristics of the metabolism and molecular biology of yeasts, and the applications of these characteristics to life in the present-dayworld ofhuman existence. The book proceeds from a brief chapter on what is and is not known of the origins and early history of the yeasts, through a description of their classification, relationships, habitats and general life style, their external morphology and internal structures and mechanisms within their cells, the regulatory mechanisms controlling processes such as signal transmis- sion, mating, cell fusion, and many others.

Автор: Stefan Hohmann; Willem H. Mager
Название: Yeast Stress Responses
ISBN: 3642078753 ISBN-13(EAN): 9783642078750
Издательство: Springer
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Автор: John F.T. Spencer; Dorothy M. Spencer; I.J. Bruce
Название: Yeast Genetics
ISBN: 3540188053 ISBN-13(EAN): 9783540188056
Издательство: Springer
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Описание: The manual consists of two main sections. The first includes the essential, sometimes laborious, procedures for handling yeasts, for inducing mating and isolation of hybrids, for inducing sporulation and isolation of single-spore clones, with some details of tetrad analysis, and including techniques and ancillary equipment for use of the micromanipulator. There are also proce- dures for induction of mutants by physical and chemical agents, and for iso- lation of particular types of mutants, such as to temperature sensitivity, for increased frequency of mutations, for mutations in the mitochondrial genome, both to the petite colonie form and to resistance to antibiotics, for mutations in that part of the yeast genome controlling the glycolytic cycle, and numerous others. Mapping of mutations is discussed briefly, though this aspect of yeast genetics is probably one which should not be undertaken until the investigator has gained a certain amount of experience in the field. How- ever, as is pointed out in the pertinent part of the manual, the task of mapping has been tremendouslysimplified by the availability from the Yeast Genetics Stock Center at the University of California at Berkeley of a set of auxo- trophic strains designed to permit mapping of most unknown genes with a minimum number of crosses and tetrad analyses. The first section concludes with the description of methods for hybridization of yeasts by protoplast fusion, which has been described as the poor man's system for genetic engineering.

Автор: Paike Jayadeva Bhat
Название: Galactose Regulon of Yeast
ISBN: 3642093302 ISBN-13(EAN): 9783642093302
Издательство: Springer
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Описание: This self contained book presents a comprehensive overview of the past, present and future of the galactose regulon of yeast, the classical model system of molecular biologists. This is followed by molecular genetics of the galactose regulon, isolation of genes and testing of the hypotheses.

Автор: Yuko Giga-Hama; Hiromichi Kumagai
Название: Foreign Gene Expression in Fission Yeast: Schizosaccharomyces pombe
ISBN: 3662034743 ISBN-13(EAN): 9783662034743
Издательство: Springer
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Цена: 18167.00 р.
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Описание: Recombinant proteins, produced in cells or microorganisms, play an important role in basic research, medical science and the biotechnology industry. Many foreign gene expression systems using mammalian cells, E. coli, insect cells, yeast and other organisms as hosts have been developed. However, only recently has the expression system of Schizosaccharomyces pombe as a host become a subject of focus, as it is expected to be profitable for expressing foreign genes. The reason for this is that S. pombe is evolutionarily more advanced than other types of yeast. This book describes the use of S. pombe in basic research and industrial applications.

Автор: Wei Xiao
Название: Yeast Protocols
ISBN: 1617375691 ISBN-13(EAN): 9781617375699
Издательство: Springer
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Описание: Basic Investigations in Saccharomyces cerevisiae Brendan P. G. Curran and Virginia Bugeja Isolation of Nucleic Acids Michelle Hanna and Wei Xiao Purification of Yeast Peroxisomes Ben Distel and Astrid Kragt Isolation of Yeast Plasma Membranes Barry Panaretou and Peter Piper Isolation of Yeast Mitochondria Chris Meisinger, Nikolaus Pfanner, and Kaye N. Truscott Extraction of Yeast Lipids Roger Schneiter and Gьnther Daum Two-Dimensional Gel Electrophoresis of Total Yeast Proteins Hйlian Boucherie and Christelle Monribot-Espagne Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes Laura Maringele and David Lydall. Analysis of Yeast Lipids, Roger Schneiter and Gьnther Daum Yeast Fluorescence Microscopy Jirн Hasek Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe Michelle A. Alting-Mees, Eddy P. Risseeuw, Enwu Liu, Michel Desautels, William A. Crosby, and Sean M. Hemmingsen Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method R. Daniel Gietz and Robin A. Woods Mutagenesis Leslie Barbour, Michelle Hanna, and Wei Xiao Gene Disruption in the Budding Yeast Saccharomyces cerevisiae Johannes H. Hegemann, Ulrich Gьldener, and Gabriele J. Kцhler Inducible Degron and Its Application to Creating Conditional Mutants R. Jьrgen Dohmen Synthetic Lethal Screen Leslie Barbour and Wei Xiao Synthetic Genetic Array Analysis in Saccharomyces cerevisiae Amy Hin Yan Tong and Charles Boone Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates Alain T. Dandjinou, Michel Larrivйe, Ralf E. Wellinger, and Raymund J. Wellinger Chromatin Assembly in a Crude Fraction From Yeast Cells Karen M.Robinson and Michael C. Schultz Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast Elena Ezhkova and William P. Tansey Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning Zhengjian Zhang and Joseph C. Reese Study of Transcriptional Regulation Using a Reporter Gene Assay Yu Fu and Wei Xiao Assessing Telomeric Phenotypes Catherine LeBel, Michel Larrivйe, Amadou Bah, Nancy Laterreur, Nancy Lйvesque, and Raymund J. Wellinger Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast Zhigang Wang Production of Heterologous Proteins in Yeast With the Aid of the Hsp150D Carrier Marja Makarow, Anna-Liisa Hдnninen, Taina Suntio, and Ricardo Nunes Bastos Yeast Two-Hybrid System Screening R. Daniel Gietz Import of Precursor Proteins Into Isolated Yeast Mitochondria Nils Wiedemann, Nikolaus Pfanner, and Peter Rehling Index

Автор: Klaus Wolf
Название: Nonconventional Yeasts in Biotechnology
ISBN: 3642798586 ISBN-13(EAN): 9783642798580
Издательство: Springer
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Описание: This is the first book to extensively and exclusively cover nonconventional yeasts - all yeasts other than S.