Описание: 1. 3D Cell Culture: An Introduction Zuzana Koledova
Part I: Hydrogels and Scaffolds for 3D Cell Culture
2. Preparation of Decellularized Biological Scaffolds for 3D Cell Culture Bryan N. Brown, Michael J. Buckenmeyer, and Travis A. Prest
3. 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix
Katrina Wisdom and Ovijit Chaudhuri
4. Hydrogel-Based In Vitro Models of Tumor Angiogenesis
Laura J. Bray, Marcus Binner, Uwe Freudenberg, and Carsten Werner
5. Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional PEG Hydrogels
Massimiliano Caiazzo, Yoji Tabata, and Matthias Lutolf
6. Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modelling in Three-Dimensions Edgar B. Montufar, Lucy Vojtova, Ladislav Celko, and Maria-Pau Ginebra
Part II: 3D Organoid and Organotypic Cultures
7. Establishment of 3D Intestinal Organoid Cultures from Intestinal Stem Cells
Shinya Sugimoto and Toshiro Sato
8. 3D Co-Culture of Mammary Organoids with Fibrospheres: A Model for Studying Epithelial-Stromal Interactions During Mammary Branching Morphogenesis
Zuzana Koledova
9. An Organotypic 3D Assay for Primary Human Mammary Epithelial Cells that Recapitulates Branching Morphogenesis
Jelena R. Linnemann, Lisa K. Meixner, Haruko Miura, and Christina H. Scheel
10. 3D Primary Culture Model to Study Human Mammary Development
Daniel H. Miller, Ethan S. Sokol, and Piyush B. Gupta
11. Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells
Anas Rabata, Ales Hampl, and Zuzana Koledova
12. 3D Hanging Drop Culture to Establish Prostate Cancer Organoids
Theresa Eder and Iris E. Eder
13. 3D Fluid-Dynamic Culture Models of Multiple Myeloma
Marina Ferrarini, Nathalie Steimberg, Jennifer Boniotti, Angiola Berenzi, Daniela Belloni, Giovanna Mazzoleni, and Elisabetta Ferrero
14. Preparation of a Three-Dimensional Full Thickness Skin Equivalent
Christian Reuter, Heike Walles, and Florian Groeber
15. Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System
Romana E. Ranftl and Fernando Calvo
16. 3D Co-Culture Model of the Brain Parenchyma/Metastasis Interface of Brain Metastasis
Raquel Blazquez and Tobias Pukrop
Part III: Micropatterning