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Cell Cycle Synchronization: Methods and Protocols, Banfalvi Gaspar


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Автор: Banfalvi Gaspar
Название:  Cell Cycle Synchronization: Methods and Protocols
ISBN: 9781493982486
Издательство: Springer
Классификация:
ISBN-10: 1493982486
Обложка/Формат: Paperback
Страницы: 354
Вес: 0.65 кг.
Дата издания: 07.07.2018
Серия: Methods in molecular biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 14 tables, color; 14 illustrations, color; 57 illustrations, black and white; xv, 354 p. 71 illus., 14 illus. in color.
Размер: 25.40 x 17.78 x 1.96 cm
Читательская аудитория: General (us: trade)
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: This second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques.


Cell Cycle Synchronization

Автор: Gaspar Banfalvi
Название: Cell Cycle Synchronization
ISBN: 1493966022 ISBN-13(EAN): 9781493966028
Издательство: Springer
Рейтинг:
Цена: 20962.00 р.
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Описание: This second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques. The chapters in Cell Cycle Synchronization: Methods and Protocols, Second Edition cover subjects such as: physical fractionations including centrifugal elutriation of healthy and apoptotic cells, and nuclei of mammalian cells; large scale mitotic cell synchronization; chromosome formation during fertilization in eggs; synchronization of unicellular organisms; hematopoietic stem cells used to improve the engraftment in transplantation; and cell cycle control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and comprehensive, Cell Cycle Synchronization: Methods and Protocols, Second Edition is a valuable resource for PhD students and postdoctoral fellows, and researchers interested in general science, pharmacy, medicine and public health, computer science, and life sciences. Specialists and professionals in cell biology, genetics, molecular biology, biochemistry, and pharmacology will also find this book useful.

Cell Cycle Synchronization

Автор: Banfalvi
Название: Cell Cycle Synchronization
ISBN: 1617791814 ISBN-13(EAN): 9781617791819
Издательство: Springer
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Цена: 23757.00 р.
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Описание: This Methods in Molecular Biology(TM) volume offers detailed protocols, with theoretical background, and step-by-step instructions on implementing synchronization, as well as current techniques for the enhanced study of regulatory mechanisms in cell cycle events.

3D Cell Culture: Methods and Protocols

Автор: Koledova Zuzana
Название: 3D Cell Culture: Methods and Protocols
ISBN: 1493983709 ISBN-13(EAN): 9781493983704
Издательство: Springer
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Цена: 17468.00 р.
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Описание: 1. 3D Cell Culture: An Introduction

Zuzana Koledova

Part I: Hydrogels and Scaffolds for 3D Cell Culture

2. Preparation of Decellularized Biological Scaffolds for 3D Cell Culture

Bryan N. Brown, Michael J. Buckenmeyer, and Travis A. Prest

3. 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix

Katrina Wisdom and Ovijit Chaudhuri

4. Hydrogel-Based In Vitro Models of Tumor Angiogenesis

Laura J. Bray, Marcus Binner, Uwe Freudenberg, and Carsten Werner

5. Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional PEG Hydrogels

Massimiliano Caiazzo, Yoji Tabata, and Matthias Lutolf

6. Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modelling in Three-Dimensions

Edgar B. Montufar, Lucy Vojtova, Ladislav Celko, and Maria-Pau Ginebra

Part II: 3D Organoid and Organotypic Cultures

7. Establishment of 3D Intestinal Organoid Cultures from Intestinal Stem Cells

Shinya Sugimoto and Toshiro Sato

8. 3D Co-Culture of Mammary Organoids with Fibrospheres: A Model for Studying Epithelial-Stromal Interactions During Mammary Branching Morphogenesis

Zuzana Koledova

9. An Organotypic 3D Assay for Primary Human Mammary Epithelial Cells that Recapitulates Branching Morphogenesis

Jelena R. Linnemann, Lisa K. Meixner, Haruko Miura, and Christina H. Scheel

10. 3D Primary Culture Model to Study Human Mammary Development

Daniel H. Miller, Ethan S. Sokol, and Piyush B. Gupta

11. Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells

Anas Rabata, Ales Hampl, and Zuzana Koledova

12. 3D Hanging Drop Culture to Establish Prostate Cancer Organoids

Theresa Eder and Iris E. Eder

13. 3D Fluid-Dynamic Culture Models of Multiple Myeloma

Marina Ferrarini, Nathalie Steimberg, Jennifer Boniotti, Angiola Berenzi, Daniela Belloni, Giovanna Mazzoleni, and Elisabetta Ferrero

14. Preparation of a Three-Dimensional Full Thickness Skin Equivalent

Christian Reuter, Heike Walles, and Florian Groeber

15. Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System

Romana E. Ranftl and Fernando Calvo

16. 3D Co-Culture Model of the Brain Parenchyma/Metastasis Interface of Brain Metastasis

Raquel Blazquez and Tobias Pukrop

Part III: Micropatterning

Neuronal Cell Culture: Methods and Protocols (2013) ( Methods in Molecular Biology #1078 )

Автор: Amini, Shohreh (Editor), White, Martyn K (Editor)
Название: Neuronal Cell Culture: Methods and Protocols (2013) ( Methods in Molecular Biology #1078 )
ISBN: 1627036393 ISBN-13(EAN): 9781627036399
Издательство: Springer
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Цена: 20962.00 р.
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Описание: This book details the latest aspects of the culture of neural cells and explains the practical and theoretical considerations of the techniques involved. It features step-by-step, readily reproducible laboratory protocols and expert implementation advice.

Natural Killer Cell Protocols: Cellular and Molecular Methods (Methods in Molecular Biology, Vol. 612)

Автор: Kerry S. Campbell
Название: Natural Killer Cell Protocols: Cellular and Molecular Methods (Methods in Molecular Biology, Vol. 612)
ISBN: 1607613611 ISBN-13(EAN): 9781607613619
Издательство: Springer
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Цена: 30745.00 р.
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Описание: Research has revealed that natural killer (NK) cells are sentinels of the innate immune response. This text offers detailed protocols plus the latest methods and techniques designed to study NK cells within specialized tissue sites in both mice and humans.

Cell Cycle Synchronization

Автор: Gaspar Banfalvi
Название: Cell Cycle Synchronization
ISBN: 1493961942 ISBN-13(EAN): 9781493961948
Издательство: Springer
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Цена: 19564.00 р.
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Описание: To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the “arrest-and-release” approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Detailed and easy to follow, Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so.

Cell Tracking: Methods and Protocols

Автор: Basel Matthew T., Bossmann Stefan H.
Название: Cell Tracking: Methods and Protocols
ISBN: 1071603639 ISBN-13(EAN): 9781071603635
Издательство: Springer
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Цена: 20962.00 р.
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Detection of Cell Death Mechanisms: Methods and Protocols

Автор: Alvero Ayesha B., Mor Gil G.
Название: Detection of Cell Death Mechanisms: Methods and Protocols
ISBN: 1071611615 ISBN-13(EAN): 9781071611616
Издательство: Springer
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Цена: 23757.00 р.
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Описание: This volume provides detailed protocols for the performance, analysis, and troubleshooting of in vitro and in vivo experiments related to programmed cell death. Chapters compile conventional techniques such as western blot and qPCR and state-of-the art transmission electron microscopy and real-time multiplexed imaging assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Detection of Cell Death Mechanisms: Methods and Protocols aims to ensure seamless execution of protocols on specific cell death type.

Cell Cycle Checkpoint Control Protocols

Автор: Howard B. Lieberman
Название: Cell Cycle Checkpoint Control Protocols
ISBN: 1617373699 ISBN-13(EAN): 9781617373695
Издательство: Springer
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Цена: 20263.00 р.
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Описание: The field of cell cycle regulation is based on the observation that the life cycle of a cell progresses through several distinct phases, G1, M, S, and G2, occurring in a well-defined temporal order.

Stem Cell Heterogeneity: Methods and Protocols

Автор: Turksen Kursad
Название: Stem Cell Heterogeneity: Methods and Protocols
ISBN: 1493982346 ISBN-13(EAN): 9781493982349
Издательство: Springer
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Цена: 15372.00 р.
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Описание: Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity.


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