Detection of Cell Death Mechanisms: Methods and Protocols, Alvero Ayesha B., Mor Gil G.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Gerst Jeffrey E. Название: RNA Detection and Visualization: Methods and Protocols ISBN: 1493958364 ISBN-13(EAN): 9781493958368 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions. In RNA Detection and Visualization: Methods and Protocols, the best and brightest investigators provide an up-to-date and in-depth description of basic methods and protocols used for detecting and visualizing mRNAs in both fixed and live cells, from bacteria to mammals. For novices and experts alike, this mix of classic in situ hybridization and advanced live imaging techniques, cell fractionation and affinity purification procedures, and bioinformatics tools gives researchers the most complete and extensive array of research aids possible. As a volume written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Detection and Visualization: Methods and Protocols offers well-honed techniques in order to inspire researchers around the world to further our knowledge of the vital biological significance of RNA.
Описание: This Methods in Molecular Biology book details approaches for the detection of DNA and RNA including recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, step-by-step, reproducible protocols, materials lists, tips and more.
Автор: Koledova Zuzana Название: 3D Cell Culture: Methods and Protocols ISBN: 1493983709 ISBN-13(EAN): 9781493983704 Издательство: Springer Рейтинг: Цена: 17468.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: 1. 3D Cell Culture: An Introduction
Zuzana Koledova
Part I: Hydrogels and Scaffolds for 3D Cell Culture
2. Preparation of Decellularized Biological Scaffolds for 3D Cell Culture
Bryan N. Brown, Michael J. Buckenmeyer, and Travis A. Prest
3. 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix
Katrina Wisdom and Ovijit Chaudhuri
4. Hydrogel-Based In Vitro Models of Tumor Angiogenesis
Laura J. Bray, Marcus Binner, Uwe Freudenberg, and Carsten Werner
5. Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional PEG Hydrogels
Massimiliano Caiazzo, Yoji Tabata, and Matthias Lutolf
6. Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modelling in Three-Dimensions
Edgar B. Montufar, Lucy Vojtova, Ladislav Celko, and Maria-Pau Ginebra
Part II: 3D Organoid and Organotypic Cultures
7. Establishment of 3D Intestinal Organoid Cultures from Intestinal Stem Cells
Shinya Sugimoto and Toshiro Sato
8. 3D Co-Culture of Mammary Organoids with Fibrospheres: A Model for Studying Epithelial-Stromal Interactions During Mammary Branching Morphogenesis
Zuzana Koledova
9. An Organotypic 3D Assay for Primary Human Mammary Epithelial Cells that Recapitulates Branching Morphogenesis
Jelena R. Linnemann, Lisa K. Meixner, Haruko Miura, and Christina H. Scheel
10. 3D Primary Culture Model to Study Human Mammary Development
Daniel H. Miller, Ethan S. Sokol, and Piyush B. Gupta
11. Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells
Anas Rabata, Ales Hampl, and Zuzana Koledova
12. 3D Hanging Drop Culture to Establish Prostate Cancer Organoids
Theresa Eder and Iris E. Eder
13. 3D Fluid-Dynamic Culture Models of Multiple Myeloma
Marina Ferrarini, Nathalie Steimberg, Jennifer Boniotti, Angiola Berenzi, Daniela Belloni, Giovanna Mazzoleni, and Elisabetta Ferrero
14. Preparation of a Three-Dimensional Full Thickness Skin Equivalent
Christian Reuter, Heike Walles, and Florian Groeber
15. Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System
Romana E. Ranftl and Fernando Calvo
16. 3D Co-Culture Model of the Brain Parenchyma/Metastasis Interface of Brain Metastasis
Raquel Blazquez and Tobias Pukrop
Part III: Micropatterning
Автор: Rainger George Edward, McGettrick Helen M. Название: T-Cell Trafficking: Methods and Protocols ISBN: 1493983423 ISBN-13(EAN): 9781493983421 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Includes bibliographical references and index.
Автор: Didenko Vladimir V. Название: Fast Detection of DNA Damage: Methods and Protocols ISBN: 149398411X ISBN-13(EAN): 9781493984114 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the “research trenches” they are not covered well in the literature. This volume is the first such compendium of the time-saving techniques for detection of DNA damage and its direct physiological outcomes including apoptosis, necrosis and phagocytic clearance. The volume demonstrates all levels of detection, starting from the molecular level up to the level of the entire live organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fast Detection of DNA Damage: Methods and Protocols aims to provide easily reproducible techniques requiring only few steps to perform.
Описание: This volume is a collection of miRNA detection and target identification protocols, detailing new developments in the traditional detection approaches such as northern blot, quantitative real-time PCR, array, next generation sequencing, and in situ hybridization.
Описание: This second edition provides techniques used to study of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. Chapters detail next-generation sequencing technologies, data analysis, protocols on analysis and visualization of single-cell RNA-Seq data, analysis of 3D chromatin architecture, interpretation of large-scale interaction networks, transcriptional networks in embryonic and adult stem cells, derivation of stem cells, and transcriptional programs that promote reprogramming, transdifferentiation, and cancer formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Stem Cell Transcriptional Network: Methods and Protocols, Second Edition aims to provide a key resource for biologists seeking to interrogate these vital networks.
Описание: This volume provides current management and research protocols on oesophageal squamous cell carcinoma.
Автор: Turksen Kursad Название: Stem Cell Nanotechnology: Methods and Protocols ISBN: 1071603590 ISBN-13(EAN): 9781071603598 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Поставка под заказ.
Описание: This fully updated edition brings together a new set of protocols to arm stem cell biologists with tools and approaches to continue the quest to uncover the intricacies and regulatory circuits underlying stem cell biology. The collection explores the tremendous progress in our understanding of the potential of stem cells and the regulatory mechanisms controlling their fate, particularly through the parallel advances in nanotechnologies and their applications in biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cell Nanotechnology: Methods and Protocols, Second Edition serves a valuable tool for experts and novices alike in the vital field of stem cell research.
Автор: Popper Zoл a. Название: The Plant Cell Wall: Methods and Protocols ISBN: 1071606190 ISBN-13(EAN): 9781071606193 Издательство: Springer Рейтинг: Цена: 34937.00 р. Наличие на складе: Поставка под заказ.
Описание: This second edition volume expands on the previous edition with many new and updated chapters discussing the latest techniques used to investigate cell wall biochemistry, biomechanical properties, chemistry, and biology.
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