Автор: Patrick Legembre Название: CD95 ISBN: 1493967789 ISBN-13(EAN): 9781493967780 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Production of the Non-apoptotic Metalloprotease-cleaved CD95L and its Cytotoxic Recombinant Counterpart Designed Ig-CD95L.- CD95 Stimulation with CD95L and DISC Analysis.- Immunoprecipitation of Death Inducing Signaling Complex by Caspase-8.- In vitro Evaluation of the Apoptosis Function in Human Activated T cells.- Proximity Ligation Assay (PLA) to Evaluate DISC and MISC Composition.- Fluorometric Methods for Detection of Mitochondrial Membrane Depolarization.- Generation and Application of Bioluminescent CD95 Ligand Fusion Proteins.- CD95-mediated Calcium Signaling.- CD95-mediated Proton Regulation.- Study of the CD95-mediated Non-apoptotic Signaling Pathway - PI3K.- Organelle Separation and Cell Signaling.- Boyden Chamber Assay to Study of Cell Migration Induced by Metalloprotease Cleaved-CD95L.- Isolation of Lipid Rafts through Discontinuous Sucrose Gradient Centrifugation and Fas/CD95 Death Receptor Localization in Raft Fractions.- Quantifying CD95/cl-CD95L Implications in Cell Mechanics And Membrane Tension by Atomic Force Microscopy Based Force Measurements.- Sketching of CD95 Oligomers by In Silico Investigations.- Site-specific Detection of Tyrosine Phosphorylated CD95 Following Protein Separation by Conventional and Phospho-Protein Affinity SDS-PAGE.- Detection of S-acylated CD95 by Acyl-biotin Exchange.- Exploration of Fas S-nitrosylation by the Biotin Switch Assay.- Method to Measure Sphingomyelin Synthase Activity Changes in Response to CD95L.- Liquid Chromatography-High Resolution Mass Spectrometry Method to Study Sphingolipid Metabolism Changes in Response to CD95L.- CD95 and the MRL-lpr Mouse Model.
Автор: Dissmeyer Nico, Schnittger Arp Название: Plant Kinases: Methods and Protocols ISBN: 149395721X ISBN-13(EAN): 9781493957217 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.
Автор: Comai Lucio, Katz Jonathan E., Mallick Parag Название: Proteomics: Methods and Protocols ISBN: 1493982907 ISBN-13(EAN): 9781493982905 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume aims to provide protocols on a wide range of biochemical methods, analytical approaches, and bioinformatics tools developed to analyze the proteome.
Автор: Rйdini Franзoise Название: Proteoglycans: Methods and Protocols ISBN: 1493958798 ISBN-13(EAN): 9781493958795 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Now recognized as a reservoir for growth factors and cytokines modulating cell activation status and turnover, proteoglycans have stimulated great amount of interest and research. In Proteoglycans: Methods and Protocols, experts in the field examine issues of basic concepts and up-to-date analysis methods for proteoglycan and glycosaminoglycan (GAG) at the protein and saccharide levels. The volume continues with the multifunctional aspect of proteoglycans, highlighted through three relevant examples of proteoglycans with highly different strucutures: serglycin, aggrecan, and heparin sulphate proteoglycans. The final chapter describes proteoglycan involvement in the pathogenesis of various disorders and their potential therapeutic value in osteo-articular diseases. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Proteoglycans: Methods and Protocols is an ideal guide for scientists attempting to pursue further research in this vital and complex area of study.
Автор: Kister Alexander E. Название: Protein Supersecondary Structures: Methods and Protocols ISBN: 1493991604 ISBN-13(EAN): 9781493991600 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This second edition volume expands on the previous edition with an update on the latest developments in the field and new techniques used to study secondary and supersecondary structures (SSS) in proteins. Chapters in this book discuss topics such as sequence and structural features of different SSS elements; software used for the automatic annotation of secondary structure elements in proteins; the new developments in secondary and SSS prediction and the modern approaches for energy landscape calculation; protein misfolding and amyloid structural formation; analysis of ‘transformer proteins’; discovery of the structure of the hydrophobic nucleus; and discussions of the main principles of protein structures formation. The contributing authors of the volume are the eminent experts in the field of protein research and bioinformatics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Protein Supersecondary Structures: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about the relationship between amino acids sequences and protein structures, the evolution of proteins and the dynamics of protein formation.
Автор: Abbott D. Wade, Lammerts Van Bueren Alicia Название: Protein-Carbohydrate Interactions: Methods and Protocols ISBN: 1493983326 ISBN-13(EAN): 9781493983322 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume is a wide-ranging tool for studying protein-carbohydrate interactions that extend from traditional biochemical methods to state-of-the-art techniques.
Автор: Lanzuolo Chiara, Bodega Beatrice Название: Polycomb Group Proteins: Methods and Protocols ISBN: 1493981811 ISBN-13(EAN): 9781493981816 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Mapping the Function of Polycomb Proteins.- Chromatin Immunoprecipitation.- Chromatin preparation and Chromatin Immuno-Precipitation from Drosophila embryos.- ChIP-seq data processing for PcG proteins and associated histone modifications.- Analysis of single locus replication timing in asynchronous cycling cells.- Non coding RNA interplay with the genome.- RIP: RNA Immunoprecipitation.- Capture Hybridization Analysis of DNA Targets.- Identification of RNA-protein interactions through in-vitro RNA pull-down assays.- Understanding RNA-chromatin interactions using Chromatin Isolation by RNA Purification (ChIRP).- Analysis RNA-seq and non coding RNA.- The dynamics of Polycomb complexes.- Fluorescence resonance energy transfer microscopy for measuring chromatin complex structure and dynamics.- Analysis of endogenous protein interactions of Polycomb-group of proteins in mouse embryonic stem cells.- Determination of Polycomb group of proteins compartmentalization through chromatin fractionation procedure.- An Automatic Segmentation Method Combining an Active Contour Model and a Classification Technique for Detecting Polycomb-group Proteins in High-Throughput Microscopy Images.- Polymer physics of the large-scale structure of chromatin.- Chromosome Conformation Capture in Drosophila.- Chromosome Conformation Capture in primary human cells.- Determination of high-resolution 3D chromatin organization using Circular Chromosome Conformation Capture (4C-seq).- Chromosome Conformation Capture on Chip (4C): Data Processing.- In vivo models to address the function of Polycomb Group Proteins.- A rapid TALEN assembly protocol.- Following the motion of Polycomb bodies in living Drosophila embryos.- Reprogramming of Somatic Cells towards Pluripotency by Cell Fusion.- Imaginal discs transplantation in Drosophila.- Isolation and culture of muscle stem cells.
Описание: This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro.
Автор: Pompa Andrea, De Marchis Francesca Название: Unconventional Protein Secretion: Methods and Protocols ISBN: 1493981447 ISBN-13(EAN): 9781493981441 Издательство: Springer Рейтинг: Цена: 6986.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I: Background
1. ER to Golgi Dependent Protein Secretion: The Conventional Pathway
Corrado Viotti
2. Unconventional Protein Secretion in Animal Cells
Fanny Ng and Bor Luen Tang
3. Unconventional Protein Secretion in Plants
Destiny J. Davis, Byung-Ho Kang, Angelo S. Heringer, Thomas E. Wilkop, and Georgia Drakakaki
Part II: UPS Contemplates Multidisciplinary Approaches for Plants
4. Chemical Secretory Pathway Modulation in Plant Protoplasts
Francesca De Marchis, Andrea Pompa, and Michele Bellucci
5. From Cytosol to the Apoplast: The Hygromycin Phosphotransferase (HYGR) Model in Arabidopsis
Haiyan Zhang and Jinjin Li
6. Following the Time-Course of Post-Pollination Events by Transmission Electron Microscopy (TEM): Buildup of Exosome-Like Structures with Compatible Pollinations
Darya Safavian, Jennifer Doucet, and Daphne R. Goring
Part III: UPS Contemplates Multidisciplinary Approaches for Animals and Fungi
7. Investigating Alternative Transport of Integral Plasma Membrane Proteins from the ER to the Golgi: Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)
Margarida D. Amaral, Carlos M. Farinha, Paulo Matos, and Hugo M. Botelho
8. Quantification of a Non-Conventional Protein Secretion: The Low Molecular Weight FGF-2 Example
Tania Arcondйguy, Christian Touriol, and Eric Lacazette
9. Human Primary Keratinocytes as a Tool for the Analysis of Caspase-1-Dependent Unconventional Protein Secretion
Gerhard E. Strittmatter, Martha Garstkiewicz, Jennifer Sand, Serena Grossi, Hans-Dietmar Beer
10. A Reporter System to Study Unconventional Secretion of Proteins Avoiding N-glycosylation in Ustilago maydis
Janpeter Stock, Marius Terfrьchte, and Kerstin Schipper
11. Stress-inducible Protein 1 (STI1): Extracellular Vesicles Analysis And Quantification
Marcos Vinicios Salles Dias, Vilma Regina Martins, and Glaucia Noeli Maroso Hajj
12. Analysis of Yeast Extracellular Vesicles
Marcio L. Rodrigues, Debora L. Oliveira, Gabriele Vargas, Wendell Girard-Dias, Anderson J. Franzen, Susana Frasйs, Kildare Miranda, and Leonardo Nimrichter
13. Exploring the Leishmania Hydrophilic Acylated Surface Protein B (HASPB) Export Pathway by Live Cell Imaging Methods
Lorna MacLean, Helen Price, and Peter O'Toole
14. Characterization of the Unconventional Secretion of the Ebola Matrix Protein VP40
Olivier Reynard and Mathieu Mateo
15. Role and Characterization of synuclein-γ Unconventional Protein Secretion in Cancer Cells
Caiyun Liu, Like Qu, and Chengchao Shou
Part IV: Secretome Isolation from Plant and Animal Samples to Identify Leaderless Secretory Proteins (LSP)
16. Characterization of the Tumor Secretome from Tumor Interstitial Fluid (TIF)
Pavel Gromov and Irina Gromova
17. Vacuum Infiltration-Centrifugation Method for Apoplastic Protein Extraction in Grapevine
Bertrand Delaunois, Fabienne Baillieul, Christophe Clйment, Philippe Jeandet, and Sylvain Cordelier
18. Isolation of Exosome-Like Vesicles from Plants by Ultracentrifugation on Sucrose/Deuterium Oxide (D2O) Density Cushions
Christopher Stanly, Immacolata Fiume, Giovambattista Capasso, and Gabriella Pocsfalvi
Автор: Pulido Rafael Название: Protein Tyrosine Phosphatases: Methods and Protocols ISBN: 1493981277 ISBN-13(EAN): 9781493981274 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vitro functional assays, suitable cell systems, and animal and microorganism models. Chapters covering state-of-the-art technical approaches suitable to decipher the physiologic roles of PTPs, and their involvement in tissue-specific functions, are also included, which will be of utility for both newcomers and experienced researchers in the field of tyrosine- and phosphoinositide- phosphorylation/dephosphorylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Tyrosine Phosphatases: Methods and Protocols aims to aid researchers in better defining the common and individual features of the PTP family members and translating this knowledge into PTP-based therapy for human disease.
Автор: Takigawa Masaharu Название: Ccn Proteins: Methods and Protocols ISBN: 1493981978 ISBN-13(EAN): 9781493981977 Издательство: Springer Рейтинг: Цена: 17468.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides a comprehensive guide on the Cyr61 (Cysteine-rich 61) (CCN) family of proteins and genes from basic research to cutting-edge methodologies and state-of-the-art techniques.
Автор: Schilling Oliver Название: Protein Terminal Profiling: Methods and Protocols ISBN: 1493983172 ISBN-13(EAN): 9781493983179 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. [14C]-Acetyl-CoenzymeA-Based In Vitro N-Terminal Acetylation Assay Adrian Drazic and Thomas Arnesen 2. DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity Hеvard Foyn, Paul R. Thompson, and Thomas Arnesen 3. SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation Willy V. Bienvenut, Carmela Giglione, and Thierry Meinnel 4. Profiling of Protein N-Termini and their Modifications in Complex Samples Fatih Demir, Stefan Niedermaier, Jayachandran N. Kizhakkedathu, and Pitter F. Huesgen 5. Protease Substrate Profiling by N-Terminal COFRADIC An Staes, Petra Van Damme, Evy Timmerman, Bart Ruttens, Elisabeth Stes, Kris Gevaert, and Francis Impens 6. Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification Benoit Westermann, Alvaro Sebastian Vaca Jacome, Magali Rompais, Christine Carapito, and Christine Schaeffer-Reiss 7. Multidimensional Analysis of Protease Substrates and their Cellular Origins in Mixed Secretomes from Multiple Cell Types Pascal Schlage and Ulrich auf dem Keller 8. System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates Zon W. Lai and Oliver Schilling 9. Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC Sebastian Tanco, Francesc Xavier Aviles, Kris Gevaert, Julia Lorenzo, and Petra Van Damme 10. ProC-TEL: Profiling of Protein C-Termini by Enzymatic Labeling Wenwen Duan and Guoqiang Xu 11. Determining Protease Substrates within a Complex Protein Background using the PROtein TOpography and Migration Analysis Platform (PROTOMAP) R.A. Fuhrman-Luck, L.M. Silva, M.L. Hastie, J.J. Gorman, J.A. Clements 12. Multiplexed Protease Specificity Profiling Using Isobaric Labeling Joanna Tucher and Andreas Tholey 13. FPPS - Fast Profiling of Protease Specificity Matej Vizovisek, Robert Vidmar, and Marko Fonovic 14. Profiling of Protease Cleavage Sites by Proteome-Derived Peptide Libraries and Quantitative Proteomics Chia-yi Chen, Bettina Mayer, and Oliver Schilling 15. Prediction of Proteases Involved in Peptide Generation Mercedes Arguello Casteleiro, Robert Stevens, and Julie Klein 16. Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches Anita Chalasani, Kyungmin Ji, Mansoureh Sameni, Samia H. Mazumder, Yong Xu, Kamiar Moin, and Bonnie F. Sloane 17. Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors Angelina Hahlbrock, Dorothйe Gц wein, and Roland H. Stauber 18. Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method Catharina Conrad, Miles A. Miller, Jцrg W Bartsch, Uwe Schlomann, Douglas A. Lauffenburger 19. Synthesis and Application of Activity-Based Probes for Proteases Tim Van Kersavond, Minh T. N. Nguyen, and Steven H. L. Verhelst
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