Cilia: Methods and Protocols, Satir Peter, Christensen Sшren Tvorup
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Chang Paul, Ohi Ryoma Название: The Mitotic Spindle: Methods and Protocols ISBN: 1493980726 ISBN-13(EAN): 9781493980727 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I Methods Focused on Cytology 1: Using Fluorescence Microscopy to Study MitosisSai K. Balchand, Barbara J. Mann, and Patricia Wadsworth 2: Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation Bin He and Daniela Cimini
Part II Methods Focused on Microtubules and the Mitotic Spindle
3: Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg Extracts Aaron C. Groen and Timothy J. Mitchison 4: Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro Marija Zanic
5: Imaging and Quantifying the Dynamics of γ-Tubulin at Microtubule Minus Ends in Mitotic Spindles Nicolas Lecland and Jens Lьders 6: Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy Matthew King and Sabine Petry 7: Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions in Synthetic Cell-Like Compartments with Tunable Size Matthew C. Good Part III Methods Focused on Kinetochores and the Kinetochore-Microtubule Interface 8: In Vitro Kinetochore Assembly Matthew D. D. Miell and Aaron F. Straight 9: Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes Tatsuya Nishino and Tatsuo Fukagawa 10: Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells Keith F. DeLuca, Jacob A. Herman, and Jennifer G. DeLuca 11: Studying Kinetochores In Vivo Using FLIM-FRET Tae Yeon Yoo and Daniel J. Needleman Section IV Methods Focused on the Spindle Pole 12: Purification of Fluorescently Labeled Saccharomyces Cerevisiae Spindle Pole Bodies Kimberly K. Fong, Beth Graczyk, and Trisha N. Davis 13: A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome Conversion Rajesh Kumar Soni and Meng-Fu Bryan Tsou 14: Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation Vladimir Joukov, Johannes C. Walter, and Arcangela De Nicolo Part V Methods Focused on the Cellular Functions of Microtubule Motor Proteins 15: Analyzing Spindle Positioning Dynamics in Cultured Cells Tomomi Kiyomitsu 16: Quantification of Mitotic Chromosome Alignment Cindy Fonseca and Jason Stumpff 17: Imaging Mitosis in the Moss Physcomitrella Patens Moй Yamada, Tomohiro Miki, and Gohta Goshima 18: Small Molecule Approach to Study the Function of Mitotic Kinesins Naowras Al-Obaidi, Johanna Kastl, and Thomas U. Mayer Part VI Novel Approaches to Study Spindle Function and Regulation 19: Identification and Characterization of Mitotic Spindle-Localized Transcripts Amy B. Emerman, Ashwini Jambhekar, and Michael D. Blower 20: Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible Dimerization Edward R. Ballister and Michael A. Lampson 21: Studying Kinetochore Kinases Adrian T. Saurin and Geert J.P.L. Kops 22: Engineering and Functional Analysis of Mitotic Kinases through Chemical Genetics Mathew J.K. Jones and Prasad V. Jallepalli Part VII The Mitotic Spindle and Cancer 23: Using Cell Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer Mijung Kwon
Автор: Goswami Ritobrata Название: Th9 Cells: Methods and Protocols ISBN: 1493983261 ISBN-13(EAN): 9781493983261 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The techniques described in Th9 Cells: Methods and Protocols study the role of Th9 cells in different inflammatory disease models, including allergic inflammation model, parasite model, tumor model, and EAE and IBD model.
Автор: Niemeyer Christof M. Название: Bioconjugation Protocols / Strategies and Methods ISBN: 1588290980 ISBN-13(EAN): 9781588290984 Издательство: Springer Цена: 11872.00 р. Наличие на складе: Поставка под заказ.
Автор: Satir Название: Cilia ISBN: 1493937871 ISBN-13(EAN): 9781493937875 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume is the result of an explosion ofmolecular-based research on Cilia, which began with the discovery of theuniversality of intraflagellar transport (IFT) and ciliary genomics/proteomics.The chapters in this book cover topics such as: high resolution imaging andfunctional characterization of sensory and primary cilia in mammalian cells andzebrafish, methods to study ciliary-mediated chemoresponse in Paramecium,and methods to study centrosomes and cilia in C. elegans and Drosophila.Written in the highly successful Methods in Molecular Biology series format,chapters include introduction to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Practical and cutting-edge, Cilia:Methods and Protocols is broad and covers motile, sensory, and primarycells. It is a valuable resource to anyone interested in entering the field ofciliary biology using model organisms, including flagellate algae, ciliates,planaria, nematodes, insects, zebrafish, and mammalian cells.
Автор: Michael Melkonian; Robert A. Andersen; Eberhard Sc Название: The Cytoskeleton of Flagellate and Ciliate Protists ISBN: 3709173914 ISBN-13(EAN): 9783709173916 Издательство: Springer Рейтинг: Цена: 19591.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Protists are fascinating organisms that have diversified and protozoologists. For example, phycologists use the evolutionarily to inhabit most of the biosphere. Their term "basal body" while protozoologists use "kine- tosome"; mycologists were indifferent and used both cytoskeleton is the primary reason that such a diver- sification is possible. Microtubules provided freedom terms from the constraint of prokaryotic cell walls and they As knowledge increased, it became more and more were organized in various cytoskeletal configurations. apparent that the organisms studied in the classical The nine pairs plus two flagellum, a major component fields did not fit into monophyletic lineages. And when of many protistan cytoskeletons, gave a new meaning efforts were made to organize the protists into a phy- to the word motility beginning at least one thousand logenetic classification, it became strikingly apparent million years ago. Associated with the development of that we needed a common vocabulary. Until such a the flagellum were new contractile and noncontractile common vocabulary exists, it will remain difficult to proteins that are necessary for control and manipu- compare studies by scientists from different fields, it lation of the flagella. As the new protists ventured out will be difficult to identify homologous structures into new habitats and filled new niches, they evolved among protists, and the historical barriers created by new ways of sensing their surrounding environment. individual fields of mycology, phycology and proto- Phototactic responses became tightly coupled with the zoology will remain.
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