This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied.
Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area.
Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.
Автор: Ricardo Simpson; Sudhir K. Sastry Название: Chemical and Bioprocess Engineering ISBN: 1493944924 ISBN-13(EAN): 9781493944927 Издательство: Springer Рейтинг: Цена: 7835.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This introductory textbook covers all aspects chemical and bioprocess engineering, and provides a thorough grounding in the fundamentals. It features more than 400 proposed and solved exercises each classified by level of difficulty.
Автор: Jean-Luc Popot Название: Membrane Proteins in Aqueous Solutions ISBN: 3030103242 ISBN-13(EAN): 9783030103248 Издательство: Springer Рейтинг: Цена: 23058.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book is the first to be entirely devoted to the challenging art of handling membrane proteins out of their natural environment, a key process in biological and pharmaceutical research, but one plagued with difficulties and pitfalls. Written by one of the foremost experts in the field, Membrane Proteins in Aqueous Solutions is accessible to any member of a membrane biology laboratory. After presenting the structure, functions, dynamics, synthesis, natural environment and lipid interactions of membrane proteins, the author discusses the principles of extracting them with detergents, the mechanisms of detergent-induced destabilization, countermeasures, and recent progress in developing detergents with weaker denaturing properties. Non-conventional alternatives to detergents, including bicelles, nanodiscs, amphipathic peptides, fluorinated surfactants and amphipols, are described, and their relative advantages and drawbacks are compared. The synthesis and solution properties of the various types of amphipols are presented, as well as the formation and properties of membrane protein/amphipol complexes and the transfer of amphipol-trapped proteins to detergents, nanodiscs, lipidic mesophases, or living cells. The final chapters of the book deal with applications: membrane protein in vitro folding and cell-free expression, solution studies, NMR, crystallography, electron microscopy, mass spectrometry, amphipol-mediated immobilization of membrane proteins, and biomedical applications.Important features of the book include introductory sections describing foundations as well as the state-of-the-art for each of the biophysical techniques discussed, and topical tables which organize a widely dispersed literature. Boxes and annexes throughout the book explain technical aspects, and twelve detailed experimental protocols, ranging from in vitro folding of membrane proteins to single-particle electron cryomicroscopy, have been contributed by and commented on by experienced users.Membrane Proteins in Aqueous Solutions offers a concise, accessible introduction to membrane protein biochemistry and biophysics, as well as comprehensive coverage of the properties and uses of conventional and non-conventional surfactants. It will be useful both in basic and applied research laboratories and as a teaching aid for students, instructors, researchers, and professionals within the field.
Автор: Bernhard Sonnleitner Название: Bioanalysis and Biosensors for Bioprocess Monitoring ISBN: 3662156385 ISBN-13(EAN): 9783662156384 Издательство: Springer Рейтинг: Цена: 36570.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: With contributions by numerous experts
Автор: Simpson Ricardo Название: Chemical and Bioprocess Engineering ISBN: 1461491258 ISBN-13(EAN): 9781461491255 Издательство: Springer Рейтинг: Цена: 8489.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This introductory textbook covers all aspects chemical and bioprocess engineering, and provides a thorough grounding in the fundamentals. It features more than 400 proposed and solved exercises each classified by level of difficulty.
Автор: Dan F. Anghel Название: Aqueous Polymer — Cosolute Systems ISBN: 3642055125 ISBN-13(EAN): 9783642055126 Издательство: Springer Рейтинг: Цена: 30606.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
A large amount of experimental data has been published since the debut of the original CRC Handbook of Thermodynamic Data of Aqueous Polymer Solutions. Incorporating new and updated material, the CRC Handbook of Phase Equilibria and Thermodynamic Data of Aqueous Polymer Solutions provides a comprehensive collection of thermodynamic data of polymer solutions. It helps readers quickly retrieve necessary information from the literature, and assists researchers in planning new measurements where data are missing.
A valuable resource for the modern chemistry field, the Handbook clearly details how measurements were conducted and methodically explains the nomenclature. It presents data essential for the production and use of polymers as well as for understanding the physical behavior and intermolecular interactions in polymer solutions.
Автор: Ferenc Jo? Название: Aqueous Organometallic Catalysis ISBN: 9048159040 ISBN-13(EAN): 9789048159048 Издательство: Springer Рейтинг: Цена: 26120.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Over the past 20 years aqueous organometallic catalysis has found applications in small- scale organic synthesis in the laboratory, as well as in the industrial production of chemicals with a combined output close to one million tons per year.
Автор: Li Название: Comprehensive Organic Reactions in Aqueous Media 2e ISBN: 047176129X ISBN-13(EAN): 9780471761297 Издательство: Wiley Рейтинг: Цена: 26286.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Organic reactions in water have proven a useful synthetic tool, and provide cost-efficient, safe, and environmentally friendly alternatives to organic-solvent based reactions. This field has been greatly expanded over the last decade, and currently there are many diverse applications in both industry and academic laboratories.
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