Oxytocin: Methods and Protocols, Werry Eryn L., Reekie Tristan A., Kassiou Michael
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Kobeissy Firas H., Dixon C. Edward, Hayes Ronald L. Название: Injury Models of the Central Nervous System: Methods and Protocols ISBN: 1493981471 ISBN-13(EAN): 9781493981472 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I Introduction
1. The History and Evolution of Experimental Traumatic Brain Injury Models John Povlishock
Part II. General Consideration in Using Animal Laboratory in CNS Injury Research 2. Clinical Traumatic Brain Injury in the Preclinical Setting Justin Berkner, Rebekah Mannix, and Jianhua Qiu 3. Rodent Models of Traumatic Brain Injury: Methods and Challenges Niklas Marklund 4.Traumatic Brain Injury Models in Animals Elham Rostami 5.Systematic Review of Traumatic Brain Injury Animal Models Helen W. Phipps 6.Methods of Drug Delivery in Neurotrauma Ying Deng-Bryant, Ryan Readnower, Lai Yee Leung, Frank Tortella, and Deborah Shear 7.Bridging the Gap of Standardized Animals Models for Blast Neurotrauma: Methodology for Appropriate Experimental Testing Pamela J. VandeVord, Alessandra Dal Cengio Leonardi, and David Ritzel 8.Cellular Mechanisms and Behavioral Outcomes in Blast-Induced Neurotrauma: Comparing Experimental Set-Ups Zachary S. Bailey, William Brad Hubbard, and Pamela J. VandeVord 9.Application of Systems Biology to Neuroproteomics: The Path to Enhanced Theranostics for Traumatic Brain Injury Zaynab Jaber, Patrick Aouad, Mohamad Al Medawar, Hisham Bahmad, Hussein Abou Abbass, and Firas Kobeissy 10.Role of Systems Biology in Brain Injury Biomarker Discovery: A Neuroproteomic Application Zaynab Jaber, Patrick Aouad, Mohamad Al Medawar, Hisham Bahmad, Hussein Abou Abbass, Hiba Ghandour, Stefania Mondello, and Firas Kobeissy Part III Classical TBI Models and Their Link with Pathophysiological Features of CNS Injury - Models 11. The Controlled Cortical Impact Model of Experimental Brain Trauma: Overview, Research Applications, and Protocol Nicole Osier and C. Edward Dixon 12.Weight Drop Models in Traumatic Brain Injury Brian T. Kalish and Michael J. Whalen 13.Midline (Central) Fluid Percussion Model of Traumatic Brain Injury Rachel K. Rowe, Daniel R. Griffiths, and Jonathan Lifshitz 14.Lateral (Parasagittal) Fluid Percussion Model of Brain Injury Ken C. Van and Bruce G. Lyeth 15.Impact Acceleration Model of Diffuse Traumatic Brain Injury Sarah C. Hellewell, Jenna M. Ziebell, Jonathan Lifshitz, and M. Cristina Morganti-Kossmann 16.Experimental Models for Neurotrauma Research Johan Davidsson and Mеrten Risling
D. Kacy Cullen, James P. Harris, Kevin D. Browne, John A. Wolf, John E. Duda, David F. Meaney, Susan S. Margulies, and Douglas H. Smith 18.Pediatric Rodent Models of Traumatic Brain Injury Bridgette D. Semple, Jaclyn Carlson, and Linda J. Noble-Haeusslein 19.Modeling Pediatric Brain Trauma: Piglet Model of Controlled Cortical Impact Jennifer C. Munoz Paraja, Kristen Keeley, Ann-Christine Duhaime, and Carter P. Dodge 20.Thromboembolic Model of Cerebral Ischemia and Reperfusion in Mice Ali Alawieh, Wenxue Wang, Aarti Narang, and Stephen Tomlinson 21.Animal Stroke Model: Ischemia/Reperfusion and Intracerebral Hemorrhage Changhong Ren, Christopher Sy, Jinhuan Gao, Yuchuan Ding, and Xunming Ji iv
Автор: Schьller Stephanie, Bielaszewska Martina Название: Shiga Toxin-Producing E. Coli: Methods and Protocols ISBN: 1071613383 ISBN-13(EAN): 9781071613382 Издательство: Springer Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides a comprehensive outline of the current methods used to detect, characterize, and investigate Shiga toxin-producing E.
Автор: Langman Loralie J., Snozek Christine L. H. Название: LC-MS in Drug Analysis: Methods and Protocols ISBN: 1493961993 ISBN-13(EAN): 9781493961993 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Liquid-Chromatography-Mass-Spectrometry procedures have been shown to be successful when applied to drug development and analysis. LC-MS in Drug Analysis: Methods and Protocols provides detailed LC-MS/MS procedures for the analysis of several compounds of clinical significance. The first chapters provide the reader with an overview of mass spectroscopy, its place in clinical practice, its application of MS to TDM and toxicology, and the merits of LC-MS(/MS) and new sample preparation techniques. The following chapters discuss different approaches to screening for drugs of abuse and for general unknowns, as well as targeted measurement of specific analytes or classes of analytes including abused drugs, toxic compounds, and therapeutic agents. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, LC-MS in Drug Analysis: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.
Автор: Marzia Martina; Stefano Taverna Название: Patch-Clamp Methods and Protocols ISBN: 1493954148 ISBN-13(EAN): 9781493954148 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. History of Electrophysiology and the Patch-Clamp
Alexej Verkhratysky and Vladimir Parpura
Part I. Pharmacology
2. Whole-Cell Patch-Clamp Analysis of Recombinant NMDA Receptor Pharmacology Using Brief Glutamate Applications
Nate Glasgow and Jon W. Johnson
3. Patch-Clamp Combined with Voltage/Concentration Clamp to Determine the Kinetics and Voltage Dependency of N-methyl-D-aspartate (NMDA) Receptor Open Channel Blockers
Chris G. Parsons and Kate E. Gilling
4. State-of-the-art Automated Patch-Clamp: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening
Sonja Stoelzle-Feix
5. Industrializing Electrophysiology: HT Automated Patch-Clamp on SyncroPatch(R) 96 Using Instant Frozen Cells
Liudmila Polonchuk
6. Planar Patch Clamp for Neuronal Networks-Considerations and Future Perspectives
Alessandro Bosca, Marzia Martina, and Christophe Py
Part II. Physiology
7. Methods for the Study of Synaptic Receptor Functional Properties
Enrica Maria Petrini and Andrea Barberis
8. Single-Cell RT-PCR, A Technique to Decipher the Electrical, Anatomical and Genetic Determinants of Neuronal Diversity
Maria Toledo-Rodriguez and Henry Markram
9. Whole-Cell Voltage-Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique
Romain Lefebvre, Sandrine Pouvreau, Claude Collet, B
runo Allard, and Vincent Jacquemond
10. Multiphoton Imaging Approaches for Studying Striatal Dendritic Excitability
Joshua L. Plotkin and James D. Surmeier
11. Investigation of Synaptic Microcircuits Using Paired Patch-Clamp Recordings in Acute Brain Slices
Giovanni Russo and Stefano Taverna
12. Outside-Out 'Sniffer-patch' Clamp Technique for in situ Measures of Neurotransmitter Release
Йmilie Muller-Chrйtien
13. A Cost Effective Method for Preparing, Maintaining and Transfecting Neurons in Organotypic Slices
Cary Soares, Kevin F.H. Lee, Denise Cook, and Jean-Claude Bйпque
14. Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics
Jonathan T. Ting, Tanya L. Daigle, Qian Chen, and Guoping Feng
15. Synaptic Connectivity in Engineered Neuronal Networks
Peter Molnar, Jung-Fong Kang, Neelima Bhargava, Mainak Das, and James J. Hickman
16. Modeling of Action Potential Generation in NG108-15 Cells
Peter Molnar and James J. Hickman
17. Whole-Cell Patch-Clamp Recordings in Freely Moving Animals
Albert K. Lee, Jйrфme Epsztein, and Michael Brecht
Part III. Biophysics
18. Pressure-Polished Borosilicate Pipettes are "Universal Sealer" Yielding Low Access Resistance and Efficient Intracellular Perfusion
Marco Aquila, Mascia B
enedusi, Anna Fasoli, and Giorgio Rispoli
19. Current Recordings at the Single Channel Level in Adult Mammalian Isolated Cardiomyocytes
Romain Guinamard, Thomas Hof, and Laurent Sallй II
20. Dynamic Clamp as a Tool to Study the Functional Effects of Individual Membrane Currents
Gйza Berecki, Arie O. Verkerk, Antoni C.G. van Ginneken, and Ronald Wilders
21. Dynamic Clamp in Cardiac and Neuronal Systems Using RTXI
Francis A. Ortega, Robert J. Butera, David J. Christini, John A. White, and Alan D. Dorval
22. Ion Selectivity of Pore-Forming Peptides and Ion Channels Measured in Xenopus Oocytes
Thierry Cens and Pierre Charnet
23. Principles of Single-Channel Kinetic Analysis
Feng Qin
Автор: Barreiro Carlos, Barredo Josй-Luis Название: Antimicrobial Therapies: Methods and Protocols ISBN: 107161360X ISBN-13(EAN): 9781071613603 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Antimicrobial resistance will become a global health threat since antimicrobial treatments continue at the forefront of the defense against microbial infections. To respond to the issue, this detailed book explores vital methodologies currently in use to advance our understanding of antibiotic issues and answer the worldwide demand for novel antibiotics therapies. Beginning with a review chapter that guides the reader through the worldwide demand for novel antibiotics therapies, the volume continues with sections covering new screening procedures and environmental sources, advances in analytical, microbiological, and biotechnological methodologies, antibiotic production and antibiotic resistances, as well as considerations of drug trials and clinical concerns regarding multi-resistant patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antimicrobial Therapies: Methods and Protocols provides a reference source for health, laboratory, and industrial professionals, as well as for graduate students in a number of bio-sanitary disciplines, including medicine, nursery, biotechnology, veterinary, microbiology, genetics, molecular biology, nutrition, farming, and more. Chapter “14” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Автор: Niopek Dominik Название: Photoswitching Proteins: Methods and Protocols ISBN: 107160757X ISBN-13(EAN): 9781071607572 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Нет в наличии.
Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.
Автор: Poulopoulos Alexandros Название: Synapse Development: Methods and Protocols ISBN: 1493982737 ISBN-13(EAN): 9781493982738 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Contributed by experts and pioneers in their respective techniques, the book covers synapses in the brain and in culture, their constituents, their structures, their dynamics, and the assemblies they form, all in the structure of a laboratory guide.
Автор: Deleyrolle Loic P. Название: Neural Progenitor Cells: Methods and Protocols ISBN: 1071617826 ISBN-13(EAN): 9781071617823 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This updated edition collects cutting-edge techniques used to study neural stem and progenitor cells as well as the brain microenvironment. Featuring a wide range of technological advances in the study of neural stem cells, the volume highlights the promises of stem cell-based therapeutic applications for central nervous system ailments. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neural Progenitor Cells: Methods and Protocols, Second Edition serves as an invaluable resource for the next generation of neuroscientists as they develop innovative experimental paradigms and progress toward therapeutic applications in the field of neurobiology.
Автор: Rasponi Marco Название: Organ-On-A-Chip: Methods and Protocols ISBN: 1071616927 ISBN-13(EAN): 9781071616925 Издательство: Springer Рейтинг: Цена: 23058.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book provides a collection of microphysiological systems employed for chemical/drug screening and strategies to mimic various physiological conditions. Chapters guide readers through Organ-on-a-Chip( OoC) platforms such as liver, intestine, blood-brain barrier, kidney, vessels, cardiac and skeletal muscles, articular joint, human fat. Additional chapters detail microfabrication technologies used to fabricate OoC devices such as, standard photo- and soft-lithography, techniques to fabricate membranes, and industrial-oriented fabrication methods.Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Organ-On-a Chip: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
Автор: Vagnoni Alessio Название: Axonal Transport: Methods and Protocols ISBN: 1071619896 ISBN-13(EAN): 9781071619896 Издательство: Springer Рейтинг: Цена: 23757.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I: Squid, Aplysia, and Xenopus
1. The Giant Axon of the Squid: A Simple System for Axonal Transport Studies
Joseph DeGiorgis, Marcus Jang, and Elaine L Bearer
2. Live Imaging and Quantitative Analysis of Organelle Transport In Sensory Neurons of Aplysia Californica
Kerriann Badal, Yibo Zhao, Kyle Miller, and Sathyanarayanan Puthanveettil
3. Live imaging of RNA Transport and Translation in Xenopus Retinal Axons
Julie Qiaojin Lin and Jean-Michel Cioni
Part II: Mouse in vivo and ex vivo
4. Imaging Axonal Transport in Ex Vivo Central and Peripheral Nerves Stacey Anne Gould, Robert Adalbert, Stefan Milde, and Michael Coleman
5. In vivo Imaging of Axonal Organelle Transport in the Mouse Brain
Johannes Knabbe, Jil Protzmann, and Thomas Kuner
6. Studying Axonal Transport in the Brain by Manganese-Enhanced Magnetic Resonance Imaging (MEMRI)
Elaine L. Bearer, Xiaowei Zhang, and Russell E. Jacobs
Part III: Rodent Primary Neurons
7. Microfluidic Neuromuscular Co-Culture System for Tracking Cell-To-Cell Transfer and Axonal Transport of Labeled Proteins
Ariel Ionescu and Eran Perlson
8. Imaging Diversity in Slow Axonal Transport
Archan Ganguly and Subhojit Roy
9. Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses
Wesley M. Tierney, Ian A. Vicino, Stella Y. Sun, Wah Chiu, Esteban A. Engel, Matthew P. Taylor, and Ian B. Hogue
10. Molecular Analysis of Axonal Transport Dynamics Upon Modulation of Microtubule Acetylation Silvia Turchetto, Romain Le Bail, Loic Broix, and Laurent Nguyen 11. Live-cell Imaging of RNA Transport In Axons Of Cultured Primary Neurons Tabitha J. Hees and Angelika B. Harbauer
12. Visualizing Vesicle-Bound Kinesins in Cultured Hippocampal Neurons
Andrew Montgomery, Alex Garbouchian, and Marvin Bentley
13. Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons.
Arman Shekari and Margaret Fahnestock
14. Use of Microfluidics Chambers to Image Axonal Transport In Adult Sensory Neurons
Maria Fransiska Emily, Lokesh Agrawal, Paolo Barzaghi, Miki Otsuki, and Marco Terenzio
Part IV: Human-derived Neurons
15. High-resolution Imaging of Mitochondria and Mitochondrial Nucleoids in Differentiated SH-SY5Y Cells
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Автор: Pfeifer Blaine A., Hill Andrew Название: Vaccine Delivery Technology: Methods and Protocols ISBN: 1071607944 ISBN-13(EAN): 9781071607947 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume discusses the vaccine development process and the role delivery concepts contribute to a global goal of effective health outcomes.
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