Photoswitching Proteins: Methods and Protocols, Niopek Dominik

Автор: Takigawa Masaharu
Название: Ccn Proteins: Methods and Protocols
ISBN: 1493981978 ISBN-13(EAN): 9781493981977
Издательство: Springer
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Цена: 17468.00 р.
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Описание: This volume provides a comprehensive guide on the Cyr61 (Cysteine-rich 61) (CCN) family of proteins and genes from basic research to cutting-edge methodologies and state-of-the-art techniques.

Автор: Garcia Fruitуs Elena, Arнs Giralt Anna
Название: Insoluble Proteins: Methods and Protocols
ISBN: 107161858X ISBN-13(EAN): 9781071618585
Издательство: Springer
Цена: 18167.00 р.
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Описание: Chapters describe recombinant protein production in different expression systems, prokaryotic and eukaryotic expression systems, purification protocols, characterization of insoluble proteins and a general overview of interesting applications of insoluble proteins.

Автор: Bhupendra Pushkar
Название: Protein Synthesis: Methods and Protocols
ISBN: 1774077035 ISBN-13(EAN): 9781774077030
Издательство: Mare Nostrum (Eurospan)
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Цена: 22730.00 р.
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Описание: Takes readers through the various ways in which proteins are synthesized and then further used for other applications. The book throws light on chemical reactions taking place in the process, and lays down different kinds of methods and the procedures that are followed in protein synthesis.

Автор: Dominik Niopek
Название: Photoswitching Proteins
ISBN: 1071607545 ISBN-13(EAN): 9781071607541
Издательство: Springer
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Цена: 15372.00 р.
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Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.

Название: Photoswitching proteins
ISBN: 1493904698 ISBN-13(EAN): 9781493904693
Издательство: Springer
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Цена: 15372.00 р.
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Описание: Photoswitching Proteins: Methods and Protocols, focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Photoswitching Proteins: Methods and Protocols highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems.

Автор: Sidney Cambridge
Название: Photoswitching Proteins
ISBN: 1493954350 ISBN-13(EAN): 9781493954353
Издательство: Springer
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Цена: 14673.00 р.
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Описание: Photoswitching Proteins Methods and Protocols, focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Photoswitching Proteins: Methods and Protocols highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems.

Автор: Lanzuolo Chiara, Bodega Beatrice
Название: Polycomb Group Proteins: Methods and Protocols
ISBN: 1493981811 ISBN-13(EAN): 9781493981816
Издательство: Springer
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Цена: 15372.00 р.
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Описание:

Mapping the Function of Polycomb Proteins.- Chromatin Immunoprecipitation.- Chromatin preparation and Chromatin Immuno-Precipitation from Drosophila embryos.- ChIP-seq data processing for PcG proteins and associated histone modifications.- Analysis of single locus replication timing in asynchronous cycling cells.- Non coding RNA interplay with the genome.- RIP: RNA Immunoprecipitation.- Capture Hybridization Analysis of DNA Targets.- Identification of RNA-protein interactions through in-vitro RNA pull-down assays.- Understanding RNA-chromatin interactions using Chromatin Isolation by RNA Purification (ChIRP).- Analysis RNA-seq and non coding RNA.- The dynamics of Polycomb complexes.- Fluorescence resonance energy transfer microscopy for measuring chromatin complex structure and dynamics.- Analysis of endogenous protein interactions of Polycomb-group of proteins in mouse embryonic stem cells.- Determination of Polycomb group of proteins compartmentalization through chromatin fractionation procedure.- An Automatic Segmentation Method Combining an Active Contour Model and a Classification Technique for Detecting Polycomb-group Proteins in High-Throughput Microscopy Images.- Polymer physics of the large-scale structure of chromatin.- Chromosome Conformation Capture in Drosophila.- Chromosome Conformation Capture in primary human cells.- Determination of high-resolution 3D chromatin organization using Circular Chromosome Conformation Capture (4C-seq).- Chromosome Conformation Capture on Chip (4C): Data Processing.- In vivo models to address the function of Polycomb Group Proteins.- A rapid TALEN assembly protocol.- Following the motion of Polycomb bodies in living Drosophila embryos.- Reprogramming of Somatic Cells towards Pluripotency by Cell Fusion.- Imaginal discs transplantation in Drosophila.- Isolation and culture of muscle stem cells.

Автор: Dos Santos Patricia C.
Название: Fe-S Proteins: Methods and Protocols
ISBN: 1071616048 ISBN-13(EAN): 9781071616048
Издательство: Springer
Цена: 18167.00 р.
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Описание: This detailed book aims to provide an overview of novel bioinformatics resources for exploring diverse aspects of RNA biology. This edition focuses on methods dealing with non-coding RNA (miRNAs, circRNAs or lncRNAs), RNA modifications (m6A or RNA editing), single cell RNA-seq and statistical models to handle count data from RNA-seq experiments.

Автор: Postis Vincent L. G., Goldman Adrian
Название: Biophysics of Membrane Proteins: Methods and Protocols
ISBN: 1071607235 ISBN-13(EAN): 9781071607237
Издательство: Springer
Цена: 31678.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.

Автор: Boudvillain Marc
Название: RNA Remodeling Proteins: Methods and Protocols
ISBN: 1071609343 ISBN-13(EAN): 9781071609347
Издательство: Springer
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Цена: 16769.00 р.
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Описание: This volume provides the most current methods to study RNA remodeling proteins.

Автор: Hobman Tom C., Duchaine Thomas F.
Название: Argonaute Proteins: Methods and Protocols
ISBN: 1493958275 ISBN-13(EAN): 9781493958276
Издательство: Springer
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Цена: 20263.00 р.
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Описание: With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner. Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels. In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research.

Автор: Kragelund Birthe B., Skriver Karen
Название: Intrinsically Disordered Proteins: Methods and Protocols
ISBN: 1071605232 ISBN-13(EAN): 9781071605233
Издательство: Springer
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Цена: 39130.00 р.
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Описание: The edition details methods to study intrinsically disordered proteins (IDPs) including recent topics such as extremely high-affinity disordered complexes, kinetics that evade established concepts, liquid-liquid phase separation, and novel disorder-driven allosteric mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Intrinsically Disordered Proteins: Methods and Protocols aims to help scientists with different backgrounds to further their investigations into these fascinating and dynamic molecules. Chapter 24 is available open access under a CC BY 4.0 license via link.springer.com. Chapters “40 and 42 ” are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.