Insoluble Proteins: Methods and Protocols, Garcia Fruitуs Elena, Arнs Giralt Anna

Автор: Elena Garc?a-Fruit?s
Название: Insoluble Proteins
ISBN: 1493946757 ISBN-13(EAN): 9781493946754
Издательство: Springer
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Цена: 15372.00 р.
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Описание: With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples.

Автор: Kragelund Birthe B., Skriver Karen
Название: Intrinsically Disordered Proteins: Methods and Protocols
ISBN: 1071605232 ISBN-13(EAN): 9781071605233
Издательство: Springer
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Цена: 39130.00 р.
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Описание: The edition details methods to study intrinsically disordered proteins (IDPs) including recent topics such as extremely high-affinity disordered complexes, kinetics that evade established concepts, liquid-liquid phase separation, and novel disorder-driven allosteric mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Intrinsically Disordered Proteins: Methods and Protocols aims to help scientists with different backgrounds to further their investigations into these fascinating and dynamic molecules. Chapter 24 is available open access under a CC BY 4.0 license via link.springer.com. Chapters “40 and 42 ” are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Автор: Hobman Tom C., Duchaine Thomas F.
Название: Argonaute Proteins: Methods and Protocols
ISBN: 1493958275 ISBN-13(EAN): 9781493958276
Издательство: Springer
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Цена: 20263.00 р.
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Описание: With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner. Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels. In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research.

Автор: Bhupendra Pushkar
Название: Protein Synthesis: Methods and Protocols
ISBN: 1774077035 ISBN-13(EAN): 9781774077030
Издательство: Mare Nostrum (Eurospan)
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Цена: 22730.00 р.
Наличие на складе: Нет в наличии.

Описание: Takes readers through the various ways in which proteins are synthesized and then further used for other applications. The book throws light on chemical reactions taking place in the process, and lays down different kinds of methods and the procedures that are followed in protein synthesis.

Автор: Boudvillain Marc
Название: RNA Remodeling Proteins: Methods and Protocols
ISBN: 1071609343 ISBN-13(EAN): 9781071609347
Издательство: Springer
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Цена: 16769.00 р.
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Описание: This volume provides the most current methods to study RNA remodeling proteins.

Автор: Postis Vincent L. G., Goldman Adrian
Название: Biophysics of Membrane Proteins: Methods and Protocols
ISBN: 1071607235 ISBN-13(EAN): 9781071607237
Издательство: Springer
Цена: 31678.00 р.
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Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.

Автор: Dos Santos Patricia C.
Название: Fe-S Proteins: Methods and Protocols
ISBN: 1071616048 ISBN-13(EAN): 9781071616048
Издательство: Springer
Цена: 18167.00 р.
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Описание: This detailed book aims to provide an overview of novel bioinformatics resources for exploring diverse aspects of RNA biology. This edition focuses on methods dealing with non-coding RNA (miRNAs, circRNAs or lncRNAs), RNA modifications (m6A or RNA editing), single cell RNA-seq and statistical models to handle count data from RNA-seq experiments.

Автор: Takigawa Masaharu
Название: Ccn Proteins: Methods and Protocols
ISBN: 1493981978 ISBN-13(EAN): 9781493981977
Издательство: Springer
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Цена: 17468.00 р.
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Описание: This volume provides a comprehensive guide on the Cyr61 (Cysteine-rich 61) (CCN) family of proteins and genes from basic research to cutting-edge methodologies and state-of-the-art techniques.

Автор: Lanzuolo Chiara, Bodega Beatrice
Название: Polycomb Group Proteins: Methods and Protocols
ISBN: 1493981811 ISBN-13(EAN): 9781493981816
Издательство: Springer
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Цена: 15372.00 р.
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Описание:

Mapping the Function of Polycomb Proteins.- Chromatin Immunoprecipitation.- Chromatin preparation and Chromatin Immuno-Precipitation from Drosophila embryos.- ChIP-seq data processing for PcG proteins and associated histone modifications.- Analysis of single locus replication timing in asynchronous cycling cells.- Non coding RNA interplay with the genome.- RIP: RNA Immunoprecipitation.- Capture Hybridization Analysis of DNA Targets.- Identification of RNA-protein interactions through in-vitro RNA pull-down assays.- Understanding RNA-chromatin interactions using Chromatin Isolation by RNA Purification (ChIRP).- Analysis RNA-seq and non coding RNA.- The dynamics of Polycomb complexes.- Fluorescence resonance energy transfer microscopy for measuring chromatin complex structure and dynamics.- Analysis of endogenous protein interactions of Polycomb-group of proteins in mouse embryonic stem cells.- Determination of Polycomb group of proteins compartmentalization through chromatin fractionation procedure.- An Automatic Segmentation Method Combining an Active Contour Model and a Classification Technique for Detecting Polycomb-group Proteins in High-Throughput Microscopy Images.- Polymer physics of the large-scale structure of chromatin.- Chromosome Conformation Capture in Drosophila.- Chromosome Conformation Capture in primary human cells.- Determination of high-resolution 3D chromatin organization using Circular Chromosome Conformation Capture (4C-seq).- Chromosome Conformation Capture on Chip (4C): Data Processing.- In vivo models to address the function of Polycomb Group Proteins.- A rapid TALEN assembly protocol.- Following the motion of Polycomb bodies in living Drosophila embryos.- Reprogramming of Somatic Cells towards Pluripotency by Cell Fusion.- Imaginal discs transplantation in Drosophila.- Isolation and culture of muscle stem cells.

Автор: Niopek Dominik
Название: Photoswitching Proteins: Methods and Protocols
ISBN: 107160757X ISBN-13(EAN): 9781071607572
Издательство: Springer
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Цена: 15372.00 р.
Наличие на складе: Поставка под заказ.

Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.

Автор: Bayry Jagadeesh
Название: The Tnf Superfamily: Methods and Protocols
ISBN: 1071611291 ISBN-13(EAN): 9781071611296
Издательство: Springer
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Цена: 18167.00 р.
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Описание: This second edition focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Chapters detail protocols on the signaling process of TNF family members, technical examples to investigating the role of TNF family members in physiopathologies, modulation of TNF signaling by pathogens, experimental applications of TNF-reporter mice, methodologies for various assays of TNF family members and the production of recombinant molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, The TNF Superfamily: Methods and Applications, Second Edition serves to aid researchers investigating this key family of proteins.

Автор: Apte Suneel S.
Название: Adamts Proteases: Methods and Protocols
ISBN: 1493997009 ISBN-13(EAN): 9781493997008
Издательство: Springer
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Цена: 18167.00 р.
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Описание: This volume provides a variety of methods used to analyze ADAMTS proteases and ADAMTS-like proteins, including their structure, substrate profile, tissue and cell distribution, post-translational modification and biological pathways. Chapters discuss genetic, cell biology, biochemical and proteomics techniques applicable to the analysis of all the members of the ADAMTS superfamily. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, ADAMTS Proteases: Methods and Protocols is a valuable tool for researchers to study both simple and sophisticated methodologies that are often difficult to locate in primary research literature.