Название: Crispr/cas9 system: applications and technology ISBN: 1536164267 ISBN-13(EAN): 9781536164268 Издательство: Nova Science Рейтинг: Цена: 13462.00 р. Наличие на складе: Невозможна поставка.
Описание: This compilation focuses on the CRISPR/Cas9 system, a genome editing tool that has been hailed as the most profound molecular biology discovery in the past decade. By employing the natural process of bacterial immunity towards bacteriophages, the tool allows researchers to precisely excise and edit parts of the genetic sequence to modify them. The authors summarize the molecular pathogenesis of hepatocellular carcinoma, available treatments/drugs and their limitations, the landscape of CRISPR targeting hepatocellular carcinoma, limitations and potential targets in future. The closing review summarises the use of CRISPR/Cas9 gene editing in ophthalmology and focuses on the advancement of gene editing in the cornea. The majority of corneal dystrophies are the result an autosomal dominant inheritance pattern within the TGFBI gene, which presents an ideal model suited for a CRISPR/Cas9 knock out methodology.
Описание: With the advent of CRISPR gene-editing technology, designer babies have become a reality. Francoise Baylis insists that scientists alone cannot decide the terms of this new era in human evolution. Members of the public, with diverse interests and perspectives, must have a role in determining our future as a species.
CRISPR in Animals and Animal Models, Volume 152, the latest release in the Progress in Molecular Biology and Translational Science series, explores the genome editing CRISPR system in cells and animal models, its applications, the uses of the CRISPR system, and the past, present and future of CRISPR genome editing. Topics of interest in this updated volume include a section on CRISPR history, The genome editing revolution, Programming CRISPR and its applications, CRISPR Delivery methods, CRISPR libraries and screening, CRISPR investigation in haploid cells, CRISPR in the generation of transgenic animals, CRISPR therapeutics, and Promising strategies and present challenges.
Автор: Stephen Tsang; George M. Church Название: Precision Medicine, CRISPR, and Genome Engineering ISBN: 331963903X ISBN-13(EAN): 9783319639031 Издательство: Springer Рейтинг: Цена: 22359.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book presents descriptive overviews of gene editing strategies across multiple species while also offering in-depth insight on complex cases of application in the field of tissue engineering and regenerative medicine.
Описание: This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.
Автор: Tsang, Stephen H. Название: Crispr Genome Surgery In Stem Cells And Disease Tissues ISBN: 0128178760 ISBN-13(EAN): 9780128178768 Издательство: Elsevier Science Рейтинг: Цена: 19875.00 р. Наличие на складе: Поставка под заказ.
Описание:
CRISPR Genome Surgery in Stem Cells and Disease Tissues focuses uniquely on the clinical applications of CRISPR/Cas9 based technology. Topics include the latest advances in gene editing and its translational applications to various diseases, including retinal degenerative disease, recessively inherited diseases, and dominantly inherited diseases, to name a few. The book's target audience includes researchers, students, clinicians and the general public. This space that is not currently served by any existing resource, so this publication fills a gap in current literature.
Автор: Magnus Lundgren Название: CRISPR : methods and protocols ISBN: 1493926861 ISBN-13(EAN): 9781493926862 Издательство: Springer Рейтинг: Цена: 23757.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.
Автор: Bailey, Scott Название: CRISPR-Cas Enzymes ISBN: 0128167602 ISBN-13(EAN): 9780128167601 Издательство: Elsevier Science Рейтинг: Цена: 24423.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: CRISPR-Cas Enzymes, Volume 616, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered in this release include CRISPR bioinformatics, A method for one-step assembly of Class 2 CRISPR arrays, Biochemical reconstitution and structural analysis of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems, Mechanistic dissection of the CRISPR interference pathway in Type I-E CRISPR-Cas system, Site-specific fluorescent labeling of individual proteins within CRISPR complexes, Fluorescence-based methods for measuring target interference by CRISPR-Cas systems, Native State Structural Characterization of CRISRP Associated Complexes using Mass Spectrometry, and more.
Автор: Jaworski L. a. Название: Hindsight: Crispr, the Last Word in Wmd ISBN: 1973673916 ISBN-13(EAN): 9781973673910 Издательство: Неизвестно Рейтинг: Цена: 5235.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Hindsight tells the riveting story of a brilliant but na ve young scientist, Katie Pendleton. Her use of gene-editing technology creates a super-seed. The super-seed is capable of feeding the world's population. Unfortunately, her research is stolen by a crazed terrorist who creates an unstoppable self-repairing super-spore capable of wiping out all agriculture. Independent of her actions, an NSA double naught spy wannabe, Larry Seabreeze, discovers the act albeit late. Separately they both work to solve their piece of the puzzle while the sitting president struggles with world events and personal betrayal.
This novel appeals to readers of all ages. Hindsight contains many irresistible elements: world-wide espionage, current events, intrigue, character growth and development.
Автор: Yonglun Luo Название: CRISPR Gene Editing ISBN: 1493991698 ISBN-13(EAN): 9781493991693 Издательство: Springer Рейтинг: Цена: 25155.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field.
Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.
Автор: DOUDNA & MALI Название: CRISPR-Cas : ISBN: 1621821315 ISBN-13(EAN): 9781621821311 Издательство: NBN International Рейтинг: Цена: 18216.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before. Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system--especially for minimizing off-target effects--are also provided. Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits.
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