DNA Electrophoresis: Methods and Protocols, Hanada Katsuhiro

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
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Цена: 5809.00 р.
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Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
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Цена: 2000.00 р.
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Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Автор: Kwok Pui-Yan
Название: Single Nucleotide Polymorphisms / Methods and Protocols
ISBN: 0896039684 ISBN-13(EAN): 9780896039681
Издательство: Springer
Цена: 5809.00 р.
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Описание: Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.

Автор: Antonio Alonso
Название: DNA Electrophoresis Protocols for Forensic Genetics
ISBN: 1493962191 ISBN-13(EAN): 9781493962198
Издательство: Springer
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Цена: 20263.00 р.
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Описание: This volume in the Methods in Molecular Biology (TM) series covers methods in forensic DNA profiling, including protocols for profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and mtDNA hypervariable regions HV1 and HV2.

Автор: Philippe Schmitt-Kopplin
Название: Capillary Electrophoresis
ISBN: 1493956655 ISBN-13(EAN): 9781493956654
Издательство: Springer
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Цена: 27950.00 р.
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Описание: Capillary Electrophoresis: Methods and Protocols presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques. The volume is intended for beginners in the field and provides overviews of each technique and a starting point for the exploration of the defined literature on the different application topics. As a beginning text, each technique is explained in simple terms, assuming only minimal previous knowledge of the methods. A Notes section accompanies each chapter and addresses problems that may occur and how to overcome them. In Part I, Analyte-Oriented, contributors present methods and protocols based on sample type and size for inorganic and organic small ions, anionic, cationic, and neutral pollutants and pharmaceuticals, sugars, amino acids, nucleotides and DNA, and synthetic copolymers up to microorganisms. In Part II, Methods-Oriented, authors present the more methodological descriptions of particular and new CE techniques including CZE, MEKC/MECC, CEC, affinity CE, coating and online concentration techniques, single-cell analysis, and multidimensional techniques. Comprehensive and timely, Capillary Electrophoresis: Methods and Protocols is ideal for researchers looking for a beginning text on capillary electrophoresis methods.

Автор: Svetlana Makovets
Название: DNA Electrophoresis
ISBN: 1627035648 ISBN-13(EAN): 9781627035644
Издательство: Springer
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Цена: 14673.00 р.
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Описание: This book details methods used to study DNA dynamics both in live cells and in test tubes. It features step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Автор: V.G. Babskii; M.Yu. Zhukov; V.I. Yudovich
Название: Mathematical Theory of Electrophoresis
ISBN: 146128225X ISBN-13(EAN): 9781461282259
Издательство: Springer
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Цена: 13060.00 р.
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Описание: The development of contemporary molecular biology with its growing tendency toward in-depth study of the mechanisms of biological processes, structure, function, and identification of biopolymers requires application of accurate physicochemical methods.

Автор: Didenko Vladimir V.
Название: Fast Detection of DNA Damage: Methods and Protocols
ISBN: 149398411X ISBN-13(EAN): 9781493984114
Издательство: Springer
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Цена: 15372.00 р.
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Описание: This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the “research trenches” they are not covered well in the literature. This volume is the first such compendium of the time-saving techniques for detection of DNA damage and its direct physiological outcomes including apoptosis, necrosis and phagocytic clearance. The volume demonstrates all levels of detection, starting from the molecular level up to the level of the entire live organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fast Detection of DNA Damage: Methods and Protocols aims to provide easily reproducible techniques requiring only few steps to perform.

Автор: Aphasizhev Ruslan
Название: RNA and DNA Editing: Methods and Protocols
ISBN: 1493958216 ISBN-13(EAN): 9781493958214
Издательство: Springer
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Цена: 16769.00 р.
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Описание: The recent expansion in diversity of RNA and DNA editing types has stimulated the development of many unique genetic, molecular, biochemical, and computational approaches to biological issues. In RNA and DNA Editing: Methods and Protocols, leading experts in the field introduce methods developed over the last few years to study editing substrates, mechanisms of specificity, and functions of RNA and DNA editing enzymes and complexes. Sections of the book are dedicated to state-of-the art techniques which enable investigation of uracil insertion/deletion RNA editing in mitochondrion of Trypanosoma brucei, adenosine to inosine RNA editing, cytidine to uracil RNA and DNA editing, as well as tRNA editing and RNA modifications. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA and DNA Editing: Methods and Protocols seeks to inspire the further development of these vital and powerful techniques.

Автор: Ke Yonggang, Wang Pengfei
Название: 3D DNA Nanostructure: Methods and Protocols
ISBN: 1493982052 ISBN-13(EAN): 9781493982059
Издательство: Springer
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Цена: 15372.00 р.
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Описание:

Part I: DNA/RNA Structure Design

Jing Pan, Tae-Gon Cha

Автор: Almeida de Sousa
Название: DNA Vaccines: Methods and Protocols
ISBN: 1071608711 ISBN-13(EAN): 9781071608715
Издательство: Springer
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Цена: 18167.00 р.
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Описание: This volume details practical procedures on the latest DNA vaccine technology. Chapters guide readers through methods and protocols on DNA vaccine design, the adjuvant influence, production and purification methodologies, delivery systems, and approaches of the influence of DNA vaccines in the immunological response performance and in the cancer immunotherapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Vaccines: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Автор: Bill Roslyn M.
Название: Recombinant Protein Production in Yeast: Methods and Protocols
ISBN: 1493958712 ISBN-13(EAN): 9781493958719
Издательство: Springer
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Цена: 15372.00 р.
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Описание: In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.