RNA and DNA Editing: Methods and Protocols, Aphasizhev Ruslan
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Kwok Pui-Yan Название: Single Nucleotide Polymorphisms / Methods and Protocols ISBN: 0896039684 ISBN-13(EAN): 9780896039681 Издательство: Springer Цена: 5809.00 р. Наличие на складе: Есть (2 шт.) Описание: Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
Автор: Hanada Katsuhiro Название: DNA Electrophoresis: Methods and Protocols ISBN: 1071603221 ISBN-13(EAN): 9781071603222 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume explores the latest techniques used by researchers to study DNA electrophoresis, with focus on various species including bacteria, yeasts, and mammalian cells. The chapters in this book cover topics such as two-dimensional gel electrophoresis; DNA replication; pulsed-field gel electrophoresis; ChIP; and post-labeling/PAGE method for detection of DNA adducts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, DNA Electrophoresis: Methods and Protocols is a valuable resource for any researchers looking to learn more about this developing field.
Автор: Hatada Izuho Название: Genome Editing in Animals: Methods and Protocols ISBN: 1493983938 ISBN-13(EAN): 9781493983933 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Construction and Evaluation of Zinc Finger Nucleases
Hiroshi Ochiai and Takashi Yamamoto
2. Current Overview of TALEN Construction Systems
Tetsushi Sakuma and Takashi Yamamoto
3. CRISPR/Cas9
Izuho Hatada and Takuro Horii
4. Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-Target Risks in Human and Mouse
Qingbo Wang and Kumiko Ui-Tei
5. Genome Editing of Mouse by Cytoplasmic Injection
Takuro Horii and Izuho Hatada
6. Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing sgRNA/Cas9 Expressing Plasmids
Taichi Noda, Asami Oji, and Masahito Ikawa
7. Genome Editing in Mouse and Rat by Electroporation
Takehito Kaneko
8. Generation of Knock-in Mouse by Genome Editing
Wataru Fujii
9. Genome Editing of Rat
Takehito Kaneko
10. Gene Targeting in Rabbits: Single-step Generation of Knockout Rabbits by Microinjection of CRISPR/Cas9 Plasmids
Yoshihiro Kawano and Arata Honda
11. Genome Editing of Pig
Masahito Watanabe and Hiroshi Nagashima
12. Genome Editing of Monkey
Zhen Liu, Yijun Cai, and Qiang Sun
13. Genome Editing Mediated by Primordial Germ Cell in Chicken
Jae Yong Han and Hong Jo Lee
14. CRISPR/Cas9-mediated Targeted Knock-in of Exogenous Reporter Genes in Zebrafish
Atsuo Kawahara
15. Genome Editing of Medaka
Satoshi Ansai and Masato Kinoshita
16. A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders using the CRISPR-Cas System
Yuto Sakane, Ken-ich T Suzuki, and Takashi Yamamoto
17. Genome Editing of Silkworms
Takuya Tsubota and Hideki Sezutsu
18. Genome Editing in the Cricket, Gryllus bimaculatus
Takahito Watanabe, Sumihare Noji, and Taro Mito
19. Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease
Yasunori Sasakura, Keita Yoshida, and Nicholas Treen
20. &
Автор: Ke Yonggang, Wang Pengfei Название: 3D DNA Nanostructure: Methods and Protocols ISBN: 1493982052 ISBN-13(EAN): 9781493982059 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I: DNA/RNA Structure Design
1. Designed 3D DNA Crystals
Nadrian C. Seeman, Ruojie Sha, Jens Birktoft, Jianping Zheng, Wenyan Liu, Tong Wang, and Chengde Mao
2. Three-Dimensional DNA Nanostructures Assembled from DNA Star Motifs
Cheng Tian and Chuan Zhang
3. Design of Wireframe DNA Nanostructures-DNA Gridiron
Dongran Han
4. Complex DNA Brick Assembly
Luvena Ong, Yonggang Ke, and Peng Yin
5. Computer-Aided Design of RNA Origami Structures
Steffen L. Sparvath, Cody W. Geary, and Ebbe S. Andersen
6. Assembling RNA Nanoparticles
Shou-Jun Xiao
Part II: Applications of DNA Structures
7. DNA Functionalization of Nanoparticles
Fang Lu and Oleg Gang
8. Purification Techniques for Three-Dimensional DNA Nanostructures
Travis A. Meyer
9. DNA Nanostructure as Smart Carriers for Drug Delivery
Xiangyuan Ouyang, Jie Chao, Shao Su, and Chunhai Fan
10. DNA G-Quadruplex-Based Assay of Enzyme Activity
Zhuoliang Liu, Kaiyu He, Wang Li, Xin Liu, Xiahong Xu, Zhou Nie, and Shouzhuo Yao
11. Assembly of Multi-Enzyme Cascade on a DNA Origami Nanostructure
Jinglin Fu and Tianran Li
12. Lipid Membrane Encapsulation of a 3D DNA NanoOctahedron
Steven D. Perrault and William M. Shih
13. DNA-PAINT Super-Resolution Imaging for Nucleic Acid Nanostructures
Mingjie Dai
14. Designing DNA Nanotube Liquid Crystals as a Weak-Alignment Medium for NMR Structure Determination of Membrane Proteins
John Min, William M. Shih, and Gaлtan Bellot
15. Direct Nanofabrication Using DNA Nanostructure
Feng Zhou and Haitao Liu
16. Confined Growth of Metal Nanoparticles Within 3D DNA Origami Molds
Wei Sun and Jie Shen
17. DNA-Directed Self-Assembly of Highly Ordered and Dense Single Walled Carbon Nanotube Arrays
Hareem Maune and Si-ping Han
18. A Proximity-Based Programmable DNA Nanoscale Assembly Line
Xiaoyan Zhang, Xiaoqiang Ding, Jianzhou Zou, and Hongzhou Gu
19. DNA Walkers as Transport Vehicles of Nanoparticles Along a Carbon Nanotube Track
Jing Pan, Tae-Gon Cha
Автор: Didenko Vladimir V. Название: Fast Detection of DNA Damage: Methods and Protocols ISBN: 149398411X ISBN-13(EAN): 9781493984114 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the “research trenches” they are not covered well in the literature. This volume is the first such compendium of the time-saving techniques for detection of DNA damage and its direct physiological outcomes including apoptosis, necrosis and phagocytic clearance. The volume demonstrates all levels of detection, starting from the molecular level up to the level of the entire live organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fast Detection of DNA Damage: Methods and Protocols aims to provide easily reproducible techniques requiring only few steps to perform.
Автор: Lusser Alexandra Название: RNA Methylation: Methods and Protocols ISBN: 1493983040 ISBN-13(EAN): 9781493983049 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides a comprehensive collection of current methods and protocols to study posttranscriptional base modifications in RNA with special focus on methylation.
Автор: Jin Hailing, Gassmann Walter Название: RNA Abundance Analysis: Methods and Protocols ISBN: 1493959166 ISBN-13(EAN): 9781493959167 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: RNA abundance analysis is one of the most important approaches for gene expression studies in the field of molecular biology. In RNA Abundance Analysis: Methods and Protocols, expert researchers cover a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. This volume includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, sulfur-containing RNAs, viral and satellite RNAs, RNA isoforms, and alternatively spliced RNA variants from various organisms, as well as key discussions of computational data processing for genome-wide datasets. Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Essential and easy to use, RNA Abundance Analysis: Methods and Protocols provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and modified RNAs, which can assist you in your gene expression studies.
Автор: Patel Tushar Название: Extracellular RNA: Methods and Protocols ISBN: 1493976516 ISBN-13(EAN): 9781493976515 Издательство: Springer Рейтинг: Цена: 11179.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Extracellular RNAs - A New Awareness of Old Perspectives
Noah Sadik, Lilian Cruz, Alessandra Gurtner, Rodosthenis S. Rodosthenous, Sophie A. Dusoswa, Olivia Ziegler, Thomas Sebastiaan Van Solinge, Zhiyun Wei, Ane Miren Salvador Garicano, Bence Gyorgy, Marike Broekman, and Leonora Balaj
2. Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles
Julia Small, Roger Alexander, and Leonora Balaj
3. Extracellular RNA Isolation from Cell Culture Supernatant
Sawen Bakr., Bridget Simonson, Kirsty M. Danielson, and Saumya Das
4. Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture
Irene K. Yan, Neha Shukla, David A. Borrelli, and Tushar Patel
5. Isolation of Extracellular RNA from Serum/Plasma
Justyna Filant, Parham Nejad, Anu Paul, Bridget Simonson, Srimeenakshi Srinivasan, Xuan Zhang, Leonora Balaj, Saumya Das, Roopali Gandhi, Louise C. Laurent, and Anil K. Sood
6. Isolation of Extracellular RNA from Bile
Irene K. Yan, Valentin X. Berdah, and Tushar Patel
7. Cushioned-Density Gradient Ultracentrifugation (C-DGUC): a Refined and High Performance Method for the Isolation, Characterization & Use of Exosomes
Kang Li, David K. Wong, King Yeung Hong, and Robert L. Raffai
8. Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids
Pete Heinzelman
9. Enrichment of Extracellular Vesicle Subpopulations via Affinity Chromatography
Michelle E. Hung, Stephen B. Lenzini, Devin M. Stranford, and Joshua N. Leonard
10. Detection and Analysis of Non-vesicular Extracellular RNA
Juan Pablo Tosar and Alfonso Cayota
11. Isolation of Plasma Lipoproteins as a Source of Extracellular RNA
Kang Li, David K. Wong, Fu Sang Luk, Roy Y. Kim, and Robert L. Raffai
12. Droplet Digital PCR for Quantitation of Extracellular RNA
Irene K Yan, Rishabh Lohray, and Tushar Patel
13. Preparation of Small RNA NGS Libraries from Biofluids
Alton Etheridge, Kai Wang, David Baxter, and David Galas
14. Multiplexed Detection and Quantitation of Extracellular Vesicle RNA Expression using NanoString
Neha Shukla, Irene K. Yan, and Tushar Patel
15. Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs
Akiko Matsuda and Tushar Patel
16. Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs
Marie-Cйcile Didiot, Reka A. Haraszti, Neil Aronin, and Anastasia Khvorova
Автор: Perez Daniel R. Название: Reverse Genetics of RNA Viruses: Methods and Protocols ISBN: 1493983539 ISBN-13(EAN): 9781493983537 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Reverse Genetics of RNA Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Автор: Napoli Sara Название: Promoter Associated RNA: Methods and Protocols ISBN: 1493982818 ISBN-13(EAN): 9781493982813 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: ChIP-seq for the Identification of Functional Elements in the Human Genome.- Identification of Candidate Functional Elements in the Genome from ChIP-seq Data.- GRO-seq, A Tool for Identification of Transcripts Regulating Gene Expression.-NanoCAGE: A Method for the Analysis of Coding and Non-coding 5'-capped Transcriptomes.- Deep Cap Analysis of Gene Expression (CAGE): Genome-Wide Identification of Promoters, Quantification of their Activity Andtranscriptional Network Inference.- Deep-RACE: Comprehensive Search for Novel ncRNAs Associated to a Specific Locus.- In silico Prediction of RNA Secondary Structure.- Computational Prediction of RNA-Protein Interactions.- Isolation of Nuclear RNA-Associated Protein Complexes.- Identification of Long Non-Coding RNAs Associated to Human Disease Susceptibility.- Targeting Promoter-associated RNAs by siRNAs.- RNA-FISH to Study Regulatory RNA at the Site of Transcription.- Detection and Characterization of R Loop Structures.- Induction of Transcriptional Gene Silencing by Expression of shRNA Directed to c-Myc P2 Promoter in Hepatocellular Carcinoma by Tissue Specific Virosomal Delivery.- Targeting Promoter Associated Non-coding RNA In Vivo.- Manipulation of Promoter-Associated Noncoding RNAs in Mouse Early Embryos for Controlling Sequence-Specific Epigenetic Status.
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