Genome Editing in Animals: Methods and Protocols, Hatada Izuho

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
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Цена: 5809.00 р.
Наличие на складе: Есть (4 шт.)
Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
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Цена: 2000.00 р.
Наличие на складе: Есть (2 шт.)
Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Автор: Kwok Pui-Yan
Название: Single Nucleotide Polymorphisms / Methods and Protocols
ISBN: 0896039684 ISBN-13(EAN): 9780896039681
Издательство: Springer
Цена: 5809.00 р.
Наличие на складе: Есть (2 шт.)
Описание: Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.

Автор: Aphasizhev Ruslan
Название: RNA and DNA Editing: Methods and Protocols
ISBN: 1493958216 ISBN-13(EAN): 9781493958214
Издательство: Springer
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Цена: 16769.00 р.
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Описание: The recent expansion in diversity of RNA and DNA editing types has stimulated the development of many unique genetic, molecular, biochemical, and computational approaches to biological issues. In RNA and DNA Editing: Methods and Protocols, leading experts in the field introduce methods developed over the last few years to study editing substrates, mechanisms of specificity, and functions of RNA and DNA editing enzymes and complexes. Sections of the book are dedicated to state-of-the art techniques which enable investigation of uracil insertion/deletion RNA editing in mitochondrion of Trypanosoma brucei, adenosine to inosine RNA editing, cytidine to uracil RNA and DNA editing, as well as tRNA editing and RNA modifications. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA and DNA Editing: Methods and Protocols seeks to inspire the further development of these vital and powerful techniques.

Автор: Lee Kiho
Название: Zygotic Genome Activation: Methods and Protocols
ISBN: 1493983598 ISBN-13(EAN): 9781493983599
Издательство: Springer
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Цена: 19564.00 р.
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Описание: This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA.

Автор: Izuho Hatada
Название: Genome Editing in Animals
ISBN: 1493971271 ISBN-13(EAN): 9781493971275
Издательство: Springer
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Цена: 19564.00 р.
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Описание:

1. Construction and Evaluation of Zinc Finger Nucleases

Hiroshi Ochiai and Takashi Yamamoto

2. Current Overview of TALEN Construction Systems

Tetsushi Sakuma and Takashi Yamamoto

3. CRISPR/Cas9

Izuho Hatada and Takuro Horii

4. Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-Target Risks in Human and Mouse

Qingbo Wang and Kumiko Ui-Tei

5. Genome Editing of Mouse by Cytoplasmic Injection

Takuro Horii and Izuho Hatada

6. Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing sgRNA/Cas9 Expressing Plasmids

20. &

Автор: William S. M. Wold; Ann E. Tollefson
Название: Adenovirus Methods and Protocols
ISBN: 1617376698 ISBN-13(EAN): 9781617376696
Издательство: Springer
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Цена: 19564.00 р.
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Описание: This updated book is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. New chapters address innovative, exciting areas of emphasis in Ad research.