RNA Methylation: Methods and Protocols, Lusser Alexandra
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Kwok Pui-Yan Название: Single Nucleotide Polymorphisms / Methods and Protocols ISBN: 0896039684 ISBN-13(EAN): 9780896039681 Издательство: Springer Цена: 5809.00 р. Наличие на складе: Есть (2 шт.) Описание: Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
Автор: Patel Tushar Название: Extracellular RNA: Methods and Protocols ISBN: 1493976516 ISBN-13(EAN): 9781493976515 Издательство: Springer Рейтинг: Цена: 11179.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Extracellular RNAs - A New Awareness of Old Perspectives
Noah Sadik, Lilian Cruz, Alessandra Gurtner, Rodosthenis S. Rodosthenous, Sophie A. Dusoswa, Olivia Ziegler, Thomas Sebastiaan Van Solinge, Zhiyun Wei, Ane Miren Salvador Garicano, Bence Gyorgy, Marike Broekman, and Leonora Balaj
2. Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles
Julia Small, Roger Alexander, and Leonora Balaj
3. Extracellular RNA Isolation from Cell Culture Supernatant
Sawen Bakr., Bridget Simonson, Kirsty M. Danielson, and Saumya Das
4. Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture
Irene K. Yan, Neha Shukla, David A. Borrelli, and Tushar Patel
5. Isolation of Extracellular RNA from Serum/Plasma
Justyna Filant, Parham Nejad, Anu Paul, Bridget Simonson, Srimeenakshi Srinivasan, Xuan Zhang, Leonora Balaj, Saumya Das, Roopali Gandhi, Louise C. Laurent, and Anil K. Sood
6. Isolation of Extracellular RNA from Bile
Irene K. Yan, Valentin X. Berdah, and Tushar Patel
7. Cushioned-Density Gradient Ultracentrifugation (C-DGUC): a Refined and High Performance Method for the Isolation, Characterization & Use of Exosomes
Kang Li, David K. Wong, King Yeung Hong, and Robert L. Raffai
8. Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids
Pete Heinzelman
9. Enrichment of Extracellular Vesicle Subpopulations via Affinity Chromatography
Michelle E. Hung, Stephen B. Lenzini, Devin M. Stranford, and Joshua N. Leonard
10. Detection and Analysis of Non-vesicular Extracellular RNA
Juan Pablo Tosar and Alfonso Cayota
11. Isolation of Plasma Lipoproteins as a Source of Extracellular RNA
Kang Li, David K. Wong, Fu Sang Luk, Roy Y. Kim, and Robert L. Raffai
12. Droplet Digital PCR for Quantitation of Extracellular RNA
Irene K Yan, Rishabh Lohray, and Tushar Patel
13. Preparation of Small RNA NGS Libraries from Biofluids
Alton Etheridge, Kai Wang, David Baxter, and David Galas
14. Multiplexed Detection and Quantitation of Extracellular Vesicle RNA Expression using NanoString
Neha Shukla, Irene K. Yan, and Tushar Patel
15. Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs
Akiko Matsuda and Tushar Patel
16. Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs
Marie-Cйcile Didiot, Reka A. Haraszti, Neil Aronin, and Anastasia Khvorova
Автор: J?rg Tost Название: DNA Methylation Protocols ISBN: 1493974793 ISBN-13(EAN): 9781493974795 Издательство: Springer Рейтинг: Цена: 27950.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This third edition volume expands on the previous editions by providing a comprehensive update on the available technologies required to successfully perform DNA methylation analysis. The different technologies discussed in this book analyze the global DNA methylation contents, comprehensive analyses using various NGS based methods for genome-wide DNA methylation analysis, along with precise quantification of DNA methylation levels on single CpG positions. The chapters in this book are divided into 7 parts: an introduction to the field along with tips on study design and data analysis; global DNA methylation levels; genome-wide DNA methylation analysis; highly multiplexed target regions; locus-specific DNA methylation analysis; DNA methylation analysis of specific biological samples; and hydroxymethylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials
and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, DNA Methylation Protocols, Third Edition is a valuable resource for postdoctoral investigators and research scientists who work with different aspects of genetics, and cellular and molecular biology, as well as clinicians who are involved in diagnostics or treatment of diseases with epigenetic components.
Автор: J?rg Tost Название: DNA Methylation Protocols ISBN: 1493984993 ISBN-13(EAN): 9781493984992 Издательство: Springer Рейтинг: Цена: 21661.00 р. Наличие на складе: Поставка под заказ.
Описание:
This third edition volume expands on the previous editions by providing a comprehensive update on the available technologies required to successfully perform DNA methylation analysis. The different technologies discussed in this book analyze the global DNA methylation contents, comprehensive analyses using various NGS based methods for genome-wide DNA methylation analysis, along with precise quantification of DNA methylation levels on single CpG positions. The chapters in this book are divided into 7 parts: an introduction to the field along with tips on study design and data analysis; global DNA methylation levels; genome-wide DNA methylation analysis; highly multiplexed target regions; locus-specific DNA methylation analysis; DNA methylation analysis of specific biological samples; and hydroxymethylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials
and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, DNA Methylation Protocols, Third Edition is a valuable resource for postdoctoral investigators and research scientists who work with different aspects of genetics, and cellular and molecular biology, as well as clinicians who are involved in diagnostics or treatment of diseases with epigenetic components.
Автор: J. Jost; H. Saluz Название: DNA Methylation ISBN: 3034899157 ISBN-13(EAN): 9783034899154 Издательство: Springer Рейтинг: Цена: 15672.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The explosive increase in the number of publications dealing with DNA methylation attests to its importance and makes it impossible to write a comprehensive coverage of the literature within the scope of a general review. Since the publication of the 3 most recent books dealing with the subject (DNA methylation by Razin A.
Автор: Jin Hailing, Gassmann Walter Название: RNA Abundance Analysis: Methods and Protocols ISBN: 1493959166 ISBN-13(EAN): 9781493959167 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: RNA abundance analysis is one of the most important approaches for gene expression studies in the field of molecular biology. In RNA Abundance Analysis: Methods and Protocols, expert researchers cover a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. This volume includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, sulfur-containing RNAs, viral and satellite RNAs, RNA isoforms, and alternatively spliced RNA variants from various organisms, as well as key discussions of computational data processing for genome-wide datasets. Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Essential and easy to use, RNA Abundance Analysis: Methods and Protocols provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and modified RNAs, which can assist you in your gene expression studies.
Автор: Perez Daniel R. Название: Reverse Genetics of RNA Viruses: Methods and Protocols ISBN: 1493983539 ISBN-13(EAN): 9781493983537 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Reverse Genetics of RNA Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Автор: Bindewald Eckart, Shapiro Bruce A. Название: RNA Nanostructures: Methods and Protocols ISBN: 1493983954 ISBN-13(EAN): 9781493983957 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume presents a collection of computational and experimental protocols pertaining to the creation, characterization, and utilization of RNA nanostructures.
Автор: Aphasizhev Ruslan Название: RNA and DNA Editing: Methods and Protocols ISBN: 1493958216 ISBN-13(EAN): 9781493958214 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The recent expansion in diversity of RNA and DNA editing types has stimulated the development of many unique genetic, molecular, biochemical, and computational approaches to biological issues. In RNA and DNA Editing: Methods and Protocols, leading experts in the field introduce methods developed over the last few years to study editing substrates, mechanisms of specificity, and functions of RNA and DNA editing enzymes and complexes. Sections of the book are dedicated to state-of-the art techniques which enable investigation of uracil insertion/deletion RNA editing in mitochondrion of Trypanosoma brucei, adenosine to inosine RNA editing, cytidine to uracil RNA and DNA editing, as well as tRNA editing and RNA modifications. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA and DNA Editing: Methods and Protocols seeks to inspire the further development of these vital and powerful techniques.
Автор: Alexandra Lusser Название: RNA Methylation ISBN: 149396805X ISBN-13(EAN): 9781493968053 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides a comprehensive collection of current methods and protocols to study posttranscriptional base modifications in RNA with special focus on methylation.
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