RNA Abundance Analysis: Methods and Protocols, Jin Hailing, Gassmann Walter

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
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Цена: 5809.00 р.
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Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
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Цена: 2000.00 р.
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Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Автор: Kwok Pui-Yan
Название: Single Nucleotide Polymorphisms / Methods and Protocols
ISBN: 0896039684 ISBN-13(EAN): 9780896039681
Издательство: Springer
Цена: 5809.00 р.
Наличие на складе: Есть (2 шт.)
Описание: Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.

Автор: Patel Tushar
Название: Extracellular RNA: Methods and Protocols
ISBN: 1493976516 ISBN-13(EAN): 9781493976515
Издательство: Springer
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Цена: 11179.00 р.
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Описание:

1. Extracellular RNAs - A New Awareness of Old Perspectives

Автор: Lusser Alexandra
Название: RNA Methylation: Methods and Protocols
ISBN: 1493983040 ISBN-13(EAN): 9781493983049
Издательство: Springer
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Цена: 19564.00 р.
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Описание: This volume provides a comprehensive collection of current methods and protocols to study posttranscriptional base modifications in RNA with special focus on methylation.

Автор: Perez Daniel R.
Название: Reverse Genetics of RNA Viruses: Methods and Protocols
ISBN: 1493983539 ISBN-13(EAN): 9781493983537
Издательство: Springer
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Цена: 15372.00 р.
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Описание: This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Reverse Genetics of RNA Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Автор: Aphasizhev Ruslan
Название: RNA and DNA Editing: Methods and Protocols
ISBN: 1493958216 ISBN-13(EAN): 9781493958214
Издательство: Springer
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Цена: 16769.00 р.
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Описание: The recent expansion in diversity of RNA and DNA editing types has stimulated the development of many unique genetic, molecular, biochemical, and computational approaches to biological issues. In RNA and DNA Editing: Methods and Protocols, leading experts in the field introduce methods developed over the last few years to study editing substrates, mechanisms of specificity, and functions of RNA and DNA editing enzymes and complexes. Sections of the book are dedicated to state-of-the art techniques which enable investigation of uracil insertion/deletion RNA editing in mitochondrion of Trypanosoma brucei, adenosine to inosine RNA editing, cytidine to uracil RNA and DNA editing, as well as tRNA editing and RNA modifications. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA and DNA Editing: Methods and Protocols seeks to inspire the further development of these vital and powerful techniques.

Автор: Napoli Sara
Название: Promoter Associated RNA: Methods and Protocols
ISBN: 1493982818 ISBN-13(EAN): 9781493982813
Издательство: Springer
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Цена: 19564.00 р.
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Описание:
ChIP-seq for the Identification of Functional Elements in the Human Genome.- Identification of Candidate Functional Elements in the Genome from ChIP-seq Data.- GRO-seq, A Tool for Identification of Transcripts Regulating Gene Expression.-NanoCAGE: A Method for the Analysis of Coding and Non-coding 5'-capped Transcriptomes.- Deep Cap Analysis of Gene Expression (CAGE): Genome-Wide Identification of Promoters, Quantification of their Activity Andtranscriptional Network Inference.- Deep-RACE: Comprehensive Search for Novel ncRNAs Associated to a Specific Locus.- In silico Prediction of RNA Secondary Structure.- Computational Prediction of RNA-Protein Interactions.- Isolation of Nuclear RNA-Associated Protein Complexes.- Identification of Long Non-Coding RNAs Associated to Human Disease Susceptibility.- Targeting Promoter-associated RNAs by siRNAs.- RNA-FISH to Study Regulatory RNA at the Site of Transcription.- Detection and Characterization of R Loop Structures.- Induction of Transcriptional Gene Silencing by Expression of shRNA Directed to c-Myc P2 Promoter in Hepatocellular Carcinoma by Tissue Specific Virosomal Delivery.- Targeting Promoter Associated Non-coding RNA In Vivo.- Manipulation of Promoter-Associated Noncoding RNAs in Mouse Early Embryos for Controlling Sequence-Specific Epigenetic Status.

Автор: Bindewald Eckart, Shapiro Bruce A.
Название: RNA Nanostructures: Methods and Protocols
ISBN: 1493983954 ISBN-13(EAN): 9781493983957
Издательство: Springer
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Цена: 20962.00 р.
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Описание: This volume presents a collection of computational and experimental protocols pertaining to the creation, characterization, and utilization of RNA nanostructures.