Yeast Genetic Networks: Methods and Protocols, Becskei Attila
Автор: Wei Xiao Название: Yeast Protocols ISBN: 1493942778 ISBN-13(EAN): 9781493942770 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Basic Investigations in Saccharomyces cerevisiae
Brendan Curran and Virginia Bugeja
2. Isolation of Yeast Nucleic Acids
Michael Biss, Michelle Hanna and Wei Xiao
3. Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry
Ting Wei and Heping Dai
4. Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method
R. Daniel Gietz
5. Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe
Johannes H. Hegemann, Sven Boris Heick, Jennifer Pцhlmann, Markus Langen and Ursula Fleig
6. See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry
Changhui Deng and Andrew N. Krutchinsky
7. Yeast Two Hybrid Liquid Screening
Elisa Donnard, Erica M. Queiroz, J. Miguel Ortega, and R. Daniel Gietz
8. Targeted Mutagenesis of a Specific Gene in Yeast
Likui Zhang, Alina Radziwon, and Linda J. Reha-Krantz
9. Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae
Kai Stefan Dimmer
10. Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy
Wei-Pang Huang, Takahiro Shintani, and Zhiping Xie
11. Assays for Autophagy II: Mitochondrial Autophagy
Tomotake Kanki and Koji Okamoto
12. Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction
Danielle Hagstrom and Changle Ma
13. Yeast Survival and Growth Assays
Xin Xu, Amanda Lambrecht, and Wei Xiao
14. Spontaneous Mutagenesis Assay
Susan Blackwell, Michelle Hanna, and Wei Xiao
15. Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene
Bernard A. Kunz
16. piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe
Jun Li and Li-Lin Du
17. Replicative and Chronological Life Span Assays
Spike D. L. Postnikoff and Troy A. A. Harkness
18. Detection of Protein Arginine Methylation in Saccharomyces cerevisiae
Christopher Aa. Jackson and Michael C. Yu
19. Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae
Lindsay G. Ball and Wei Xiao
20. Ligand Engineering Using Yeast Surface Display
Benjamin J. Hackel
21. Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay
Kris Barreto and C. Ronald Geyer
Автор: Alistair J.P. Brown; Mick F. Tuite; John E.G. McCa Название: Protein Synthesis and Targeting in Yeast ISBN: 3642849237 ISBN-13(EAN): 9783642849237 Издательство: Springer Рейтинг: Цена: 16979.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Proceedings of the NATO Advanced Research Workshop on Protein Synthesis and Targeting in Yeast, held at Canterbury, Kent, UK, July 4-8, 1992
Автор: Watson Dean Название: Microbiology: Yeast and Fungi ISBN: 1682861481 ISBN-13(EAN): 9781682861486 Издательство: Неизвестно Цена: 23333.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book contains some path-breaking studies in the field of microbiology with particular emphasis on yeast and fungi. It traces the progress of this field and highlights some of its key concepts and applications. The chapters included in this book focus on structures and classification of yeast and fungi, their physiology, applications of yeast and fungi in different fields that of utmost significance and bound to provide incredible insights to readers. Some of the diverse topics covered in this book address the varied branches that fall under this category. The book aims to provide a comprehensive overview of the discipline as well as the recent developments and researches in the field of yeast and fungal science.
Описание: In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.
Автор: Wei Xiao Название: Yeast Protocols ISBN: 1493907980 ISBN-13(EAN): 9781493907984 Издательство: Springer Рейтинг: Цена: 20962.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Автор: Xiao Wei Название: Yeast Protocols ISBN: 1071608673 ISBN-13(EAN): 9781071608678 Издательство: Springer Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Human trafficking is one of the most pressing social justice issues of our time. Though renewed interest in this issue among Christians is a wonderful thing, misinformed and misguided efforts can do more harm than good. Written by seasoned leaders and grounded in theology and up-to-date data, this accessible and compelling handbook will educate churches and organizations for truly effective work.
Автор: Mitsuyoshi Ueda Название: Yeast Cell Surface Engineering ISBN: 9811358672 ISBN-13(EAN): 9789811358678 Издательство: Springer Рейтинг: Цена: 13974.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book provides a detailed and up-to-date overview of all aspects of yeast cell surface engineering, including fundamental principles, practical strategies for the construction of engineered yeasts, as well as medical and industrial applications. The technique makes it possible to add eukaryotic modifications to the surface-displayed proteins/peptides, which is of significant value in basic and applied research. Generally referred to as an arming (molecular display) technology, it allows yeast to be used as a whole-cell biocatalyst for a range of purposes, including bio-energy production, pollutant removal, recovery of rare metal ions, and preparation of functional cells, all of which are comprehensively covered in the book. Among the medical applications discussed are in vitro antibody preparation and the production of oral vaccines. In addition, it presents the latest advances in protein engineering and high-throughput screening for directed evolution of enzymes. The book enables graduate students and researchers to gain a deeper, comprehensive understanding of the technology, and offers further inspiration for researchers and industrial experts in this rapidly evolving field.
Автор: Stephen G. Oliver; Juan I. Castrillo Название: Yeast Systems Biology ISBN: 1493997351 ISBN-13(EAN): 9781493997350 Издательство: Springer Рейтинг: Цена: 21661.00 р. Наличие на складе: Поставка под заказ.
Описание: This second edition volume expands on the previous edition with a look at the latest advances in techniques to study yeast and its core set of interactions, modules, architectures, and network dynamics that are common in all eukaryotes. The chapters in this book are organized into Four Parts: Part One provides readers with an update on the development of novel experimental and computational approaches to yeast systems biology; Part Two explores high-throughput methods used to study yeast epigenome, transcriptome, proteome, and metabolome; Part Three talks about computational systems biology, and focuses on data management, dynamic modeling, constraint-based models of metabolic networks, and multi-level ‘omics data; while Part Four looks at experimental platforms that utilize yeast to model systemic human diseases such as Alzheimer’s and Parkinson’s diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step, readily reproducible laboratory protocols; and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Yeast System Biology: Methods and Protocols, Second Edition is a valuable tool for graduate students, post-doctoral researchers, and experts who are interested in learning about the latest developments in the study of yeast.
Автор: Jure Pi?kur; Concetta Compagno Название: Molecular Mechanisms in Yeast Carbon Metabolism ISBN: 366251222X ISBN-13(EAN): 9783662512227 Издательство: Springer Рейтинг: Цена: 19589.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Introduction to Carbon Metabolism in Yeast.- Glucose Sensing and Signal Transduction in Saccharomyces cerevisiae.- Anaerobic Carbon Metabolism of Saccharomyces cerevisiae.- Systems Biology: Developments and Applications.- Comparative Genomics and Evolutionary Genetics of Yeast Carbon Metabolism.- Similarities and Differences Between Cancer and Yeast Carbohydrate Metabolism.- Carbon Metabolism in Pathogenic Yeasts (Especially Candida): The Role of Cell Wall Metabolism in Virulence.- Molecular Mechanisms in Yeast Carbon Metabolism: Lipid Metabolism and Lipidomics.- Molecular Mechanisms in Yeast Carbon Metabolism: Bioethanol and Other Biofuels.- Wine, Beer and Cider: Unravelling the Aroma Profile.- Production of Metabolites and Heterologous Proteins.
Автор: Stephen G. Oliver; Juan I. Castrillo Название: Yeast Systems Biology ISBN: 1493997386 ISBN-13(EAN): 9781493997381 Издательство: Springer Рейтинг: Цена: 21661.00 р. Наличие на складе: Поставка под заказ.
Описание: This second edition volume expands on the previous edition with a look at the latest advances in techniques to study yeast and its core set of interactions, modules, architectures, and network dynamics that are common in all eukaryotes. The chapters in this book are organized into Four Parts: Part One provides readers with an update on the development of novel experimental and computational approaches to yeast systems biology; Part Two explores high-throughput methods used to study yeast epigenome, transcriptome, proteome, and metabolome; Part Three talks about computational systems biology, and focuses on data management, dynamic modeling, constraint-based models of metabolic networks, and multi-level ‘omics data; while Part Four looks at experimental platforms that utilize yeast to model systemic human diseases such as Alzheimer’s and Parkinson’s diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step, readily reproducible laboratory protocols; and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Yeast System Biology: Methods and Protocols, Second Edition is a valuable tool for graduate students, post-doctoral researchers, and experts who are interested in learning about the latest developments in the study of yeast.
Автор: Valeria Mapelli Название: Yeast Metabolic Engineering ISBN: 1493952862 ISBN-13(EAN): 9781493952861 Издательство: Springer Рейтинг: Цена: 14673.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications.
Автор: Graham G. Stewart Название: Distilling and Brewing Yeast ISBN: 3319691244 ISBN-13(EAN): 9783319691244 Издательство: Springer Рейтинг: Цена: 30745.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Yeast stress, vitality and viability are also addressed together with flavor production, genetic manipulation, bioethanol formation and ethanol production by non-Saccharomyces yeasts and a Gram-negative bacterium.
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