Wnt Signaling: Methods and Protocols, Barrett Quinn, Lum Lawrence

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
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Цена: 5809.00 р.
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Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
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Цена: 2000.00 р.
Наличие на складе: Есть (2 шт.)
Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Автор: Banafsh? Larijani; Rudiger Woscholski; Colin A. Ro
Название: Lipid Signaling Protocols
ISBN: 1627038825 ISBN-13(EAN): 9781627038829
Издательство: Springer
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Цена: 20263.00 р.
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Описание: In a single volume this book assembles the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function. This is an essential resource for both experienced and novice researchers.

Автор: Seger Rony
Название: Map Kinase Signaling Protocols
ISBN: 1493961055 ISBN-13(EAN): 9781493961054
Издательство: Springer
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Цена: 15372.00 р.
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Описание: Exploring the activation and function of components of the MAPK signaling cascades, this volume presents fully updated techniques to study MAPK. Chapters include lists of the necessary materials, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Автор: Feng Xin-Hua, Xu Pinglong, Lin Xia
Название: Tgf-β Signaling: Methods and Protocols
ISBN: 1493949934 ISBN-13(EAN): 9781493949939
Издательство: Springer
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Цена: 16070.00 р.
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Описание: Chapters provide basic introduction of TGF-ss signaling from the cell surface to the nucleus, methods and techniques for the investigation of TGF-ss signaling mechanism including receptors, Smads, intracellular kinases, microRNA, epigenetic regulation, post-translational regulations, non-Smad pathway;

Автор: Jamila I. Horabin
Название: Hedgehog Signaling Protocols
ISBN: 161737721X ISBN-13(EAN): 9781617377211
Издательство: Springer
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Цена: 21661.00 р.
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Описание: Intended for Molecular Biologists, Biochemists, and Geneticists interested in manipulating and analyzing the Hedgehog Signaling Pathway, this volume offers various detailed chapters on experimental description, including anecdotal pointers and notes.

Автор: Barrett
Название: Wnt Signaling
ISBN: 1493963910 ISBN-13(EAN): 9781493963911
Издательство: Springer
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Цена: 19564.00 р.
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Описание: Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues from induced pluripotent and embryonic stem cells, monitoring Wnt transcriptional responses in diverse tissues such as bone and skin, and using specific biochemical markers of Wnt signaling to either screen molecular libraries or evaluate novel reagents. These protocols also leverage unique experimental strengths from five different model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Автор: Mark Waugh
Название: Lipid Signaling Protocols
ISBN: 149395007X ISBN-13(EAN): 9781493950072
Издательство: Springer
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Цена: 13974.00 р.
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Описание:

1. Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase α in Quantitative High-Throughput Screening (qHTS) Bioluminescent Format

Mindy I. Davis, Atsuo T. Sasaki, and Anton Simeonov

2. Assaying Ceramide Synthase Activity In Vitro and in Living Cells using Liquid Chromatography-Mass Spectrometry

Xin Ying Lim, Russell Pickford, and Anthony S Don

3. Fluorescent Assays for Ceramide Synthase Activity

Timothy A Couttas and Anthony S Don

4. Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-kinase type II Alpha

Avanti Gokhale, Pearl V. Ryder, Stephanie A. Zlatic, and Victor Faundez

5. Measurement of Long Chain Fatty Acyl-CoA synthetase Activity

Joachim Fьllekrug and Margarete Poppelreuther

6. Qualitative and Quantitative in vitro Analysis of Phosphatidylinositol Phosphatase Substrate Specificity

Laura Ren Huey Ip and Christina Anja Gewinner

7. Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity

Matthew C. Gage, Benoit Pourcet, and Inйs Pineda-Torra

8. Metabolically Biotinylated Reporters for Electron Microscopic Imaging of Cytoplasmic Membrane Microdomains

Kimberly J. Krager and John G. Koland

9. Fluorescence Recovery after Photobleaching Analysis of the Diffusional Mobility of Plasma Membrane Proteins: HER3 Mobility in Breast Cancer Cell