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Lysosomes: Methods and Protocols, Цllinger Karin, Appelqvist Hanna


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Автор: Цllinger Karin, Appelqvist Hanna
Название:  Lysosomes: Methods and Protocols
ISBN: 9781493983438
Издательство: Springer
Классификация:
ISBN-10: 1493983431
Обложка/Формат: Paperback
Страницы: 311
Вес: 0.57 кг.
Дата издания: 20.07.2018
Серия: Methods in molecular biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 61 illustrations, color; 17 illustrations, black and white; xiii, 311 p. 78 illus., 61 illus. in color.
Размер: 25.40 x 17.78 x 1.75 cm
Читательская аудитория: Professional & vocational
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
Рейтинг:
Поставляется из: Германии
Описание:

1. SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS
Melanie Thelen, Dominic Winter, Thomas Braulke, and Volkmar Gieselmann

2. Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics
Mesut Bilgin, Jesper Nylandsted, Marja Jддttelд, and Kenji Maeda

3. Analysis of N- And O-Glycosylation of Lysosomal Glycoproteins
Elmira Tokhtaeva, Olga A. Mareninova, Anna S. Gukovskaya, and Olga Vagin

4. Analyzing Lysosome Related Organelles By Electron Microscopy
Ilse Hurbain, Maryse Romao, Ptissam Bergam, Xavier Heiligenstein, And Graзa Raposo

5. Microscopic Analysis of Lysosomal Membrane Permeabilization
Ana Maria Vilamill Giraldo, Karin Цllinger, and Vesa Loitto

6. Quantitative Co-localization and Pattern Analysis Of Endo-Lysosomal Cargo in Subcellular Image Cytometry and Validation on Synthetic Image Sets
Frederik W. Lund and Daniel Wьstner

7. Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tissues
Mingguang Ren, Beibei Deng, Xiuqi Kong, Yonghe Tang, and Weiying Lin

8. Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy Dependent Recovery
Takanobu Otomo and Tamotsu Yoshimori

9. Delivery of Cargo to Lysosomes using Gneosomes
Kristina M. Hamill, Ezequiel Wexselblatt, Wenyong Tong, Jeffrey D. Esko, and Yitzhak Tor

10. Lysosomal Acidification in Cultured Astrocytes Using Nanoparticles
Camilla Lццv and Anna Erlandsson

11. Analysis of Lysosomal pH by Flow Cytometry Using FITC-dextran Loaded Cells
Ida Eriksson, Karin Цllinger, and Hanna Appelqvist

12. Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry
Anna L. Sцdergren and Sofia Ramstrцm

13. Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes
Norma W. Andrews

14. Using the MEROPS Database for Investigation of Lysosomal Peptidases, their Inhibitors and Substrates
Neil D. Rawlings

15. Next Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Human Disease: The Example of Lysoplex
Giuseppina Di Fruscio, Sandro Banfi, Vincenzo Nigro, and Andrea Ballabio

16. Gelatin Zymography using Leupeptin for the Detection of Various Cathepsin L Forms
Yoko Hashimoto

17. Methods for Determination of Α-Glycosidase, Β-Glycosidase, and Α-Galactosidase Activities in Dried Blood Spot Samples
Eser Yıldırım Sozmen and Ebru Demirel Sezer

18. Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi
Jyotsna Vermaa, Sunita Bijarnia-Mahaya, and Ishwar C. Vermaa

19. Lysosomal Biology in Cancer
Colin Fennelly and Ravi Amaravadi






Antibiotic Resistance Protocols

Автор: Steven H. Gillespie, Timothy D. McHugh
Название: Antibiotic Resistance Protocols
ISBN: 160327278X ISBN-13(EAN): 9781603272780
Издательство: Springer
Рейтинг:
Цена: 13695.00 р. 19564.00 -30%
Наличие на складе: Есть (1 шт.)
Описание: This book fully updates and builds upon its first edition. Beginning with chapters on epidemiology and population genetics, it continues with sections covering genomics and gene expressions, fitness mutation and physiology, and the detection of resistance.

Affinity Chromatography / Methods and Protocols

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
Рейтинг:
Цена: 2000.00 р.
Наличие на складе: Есть (2 шт.)
Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

In Situ Detection of DNA Damage / Methods and Protocols

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
Рейтинг:
Цена: 5809.00 р.
Наличие на складе: Есть (4 шт.)
Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Matrix Metalloproteinase Protocols

Автор: Ian M. Clark
Название: Matrix Metalloproteinase Protocols
ISBN: 1603272984 ISBN-13(EAN): 9781603272988
Издательство: Springer
Рейтинг:
Цена: 14673.00 р. 20962.00 -30%
Наличие на складе: Есть (1 шт.)
Описание: Detailing proven laboratory techniques for the study of MMPs, this book covers the methods including those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a variety of circumstances.

Human Embryonic Stem Cell Protocols

Автор: Turksen
Название: Human Embryonic Stem Cell Protocols
ISBN: 1607613689 ISBN-13(EAN): 9781607613688
Издательство: Springer
Рейтинг:
Цена: 14673.00 р. 20962.00 -30%
Наличие на складе: Есть (1 шт.)
Описание: Controversy surrounds the study of human embryonic stem cells. In this book, respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells.

Lysosomes

Автор: Eric Holtzman
Название: Lysosomes
ISBN: 0306429667 ISBN-13(EAN): 9780306429668
Издательство: Springer
Рейтинг:
Цена: 34799.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: In 1976 I wrote a monograph on lysosomes (Lysosomes: A Survey, Springer- Verlag, Vienna) that was intended as an up-to-date, comprehensive survey. My goal here, therefore, is simply to introduce the major features of lysosomes at a level I hope will be useful both to I;ldvanced students and to researchers interested in obtaining a broad background.

3D Cell Culture

Автор: Zuzana Koledova
Название: 3D Cell Culture
ISBN: 1493970194 ISBN-13(EAN): 9781493970193
Издательство: Springer
Рейтинг:
Цена: 17468.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, 3D Cell Culture: Methods and Protocols aims to inspire researchers to develop novel 3D cell culture techniques according to their specific scientific needs and interests, leading to a new generation of physiologically relevant and realistic 3D cell cultures.
Chapter 15 of this book is available open access under a CC BY 4.0 license.

Inositol Phosphates

Автор: Gregory J. Miller
Название: Inositol Phosphates
ISBN: 1071601660 ISBN-13(EAN): 9781071601662
Издательство: Springer
Рейтинг:
Цена: 15372.00 р.
Наличие на складе: Поставка под заказ.

Описание: This volume describes modern approaches for the detection and analysis of inositol phosphates from various biological, clinical, and environmental samples. Chapters focus on methods to study enzymes that modify inositol signals, chemical biology of inositol phosphates, and methods to synthesize and purify inositol phosphates and their analogs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Inositol Phosphates: Methods and Protocol aims to ensure successful results in the further study of this vital field.

Heterologous Expression of Membrane Proteins

Автор: Isabelle Mus-Veteau
Название: Heterologous Expression of Membrane Proteins
ISBN: 1493981021 ISBN-13(EAN): 9781493981021
Издательство: Springer
Рейтинг:
Цена: 13974.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs).

Radiation Cytogenetics

Автор: Takamitsu A. Kato; Paul F. Wilson
Название: Radiation Cytogenetics
ISBN: 1493994301 ISBN-13(EAN): 9781493994304
Издательство: Springer
Рейтинг:
Цена: 22359.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA damage in cells, but it is also a standard biological method to estimate human radiation exposure in the radiation accidents. Therefore, we prepared a series of chapters not only covering biological aspects but also exploring radiation physics and radiation protection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Radiation Cytogenetics: Methods and Protocols serves both novices working on their first experiments as well as experienced researchers searching for tried-and-true techniques to adapt for their own laboratory.
Cell-Cell Interactions

Автор: Troy A. Baudino
Название: Cell-Cell Interactions
ISBN: 1493960490 ISBN-13(EAN): 9781493960491
Издательство: Springer
Рейтинг:
Цена: 14673.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This book details protocols to examine interactions between cells, including those that focus on both in vivo and in vitro methods-based approaches. It provides step-by-step detail essential for reproducible results and contains key implementation advice.

Neural Crest Cells

Автор: Quenten Schwarz; Sophie Wiszniak
Название: Neural Crest Cells
ISBN: 149399414X ISBN-13(EAN): 9781493994144
Издательство: Springer
Рейтинг:
Цена: 15372.00 р.
Наличие на складе: Поставка под заказ.


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