Histones: Methods and Protocols, Guillemette Benoit, Gaudreau Luc R.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Stock Peggy, Christ Bruno Название: Hepatocyte Transplantation: Methods and Protocols ISBN: 1493982214 ISBN-13(EAN): 9781493982219 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume features up-to-date protocols for the isolation, preservation, and validation of various cell sources comprising large and small animal models, examining the impact of cell transplantation on acute and chronic liver diseases.
Автор: Stephen Misener; Stephen A. Krawetz Название: Bioinformatics Methods and Protocols ISBN: 1617371564 ISBN-13(EAN): 9781617371561 Издательство: Springer Рейтинг: Цена: 23058.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: We divided Bioinformatics Methods and Protocols into five parts, including a thorough survey of the basic sequence analysis software packages that are available at most institutions, as well as the design and implemen- tion of an essential introductory Bioinformatics course.
Автор: Costa Cristina, Mбсez Rafael Название: Xenotransplantation: Methods and Protocols ISBN: 1493958690 ISBN-13(EAN): 9781493958696 Издательство: Springer Рейтинг: Цена: 20263.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In this book, experts study characterizations of xenogeneic interactions at the cellular and molecular levels and describe the use of relevant small-animal and pig-to-primate models. It also covers related ethical and legal considerations.
Автор: Duque Paula Название: Environmental Responses in Plants: Methods and Protocols ISBN: 1493980297 ISBN-13(EAN): 9781493980291 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
PaPart I Tropisms
1.11. Hydrotropism: Analysis of the Root Response to a Moisture Gradient Regina Antoni, Daniela Dietrich, Malcolm J. Bennett, and Pedro L. Rodriguez
2. Assessing Gravitropic Responses in Arabidopsis Richard Barker, Benjamin Cox, Logan Silber, Arah Sangari, Amir Assadi, and Patrick Masson
3. Physiological Analysis of Phototropic Responses in Arabidopsis Mathias Zeidler
4. Automatic Chloroplast Movement Analysis Henrik Johansson and Mathias Zeidler
5. Micro
scopic and Biochemical Visualization of Auxins in Plant Tissues Joshua J. Blakeslee and Angus S. Murphy
6. Immunolocalization of PIN and ABCB Transporters in Plants Nicola Carraro and Wendy A. Peer
Part II Photoperiodism and Circasian Rhythms
7.Analysis of Circadian Leaf Movements Niels A. Mьller and Josй M. Jimйmez-Gуmez
8. Sample Preparation of Arabidopsis Thaliana Shoot Apices for Expression Studies of Photoperiod-Induced Genes Fernando Andrйs, Stefano Torti, Coral Vincent, and George Coupland
9. A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs are
Temporally Restricted by the Circadian Clock Amaury de Montaigu, Markus C. Berns, and Geroge Coupland
10. Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hyrbid Screens Using a Transcription Factor ORFeome Ghislain Breton, Steve A. Kay, and Josй L. Pruneda-Paz
11. Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes Craig G. Simpson, John Fuller, Cristiane P. G. Calixto, Jim McNicol, Clare Booth, John W. S. Brown, and Dorothee Staiger
12. Assessing the Impact of Photosynthetic Sugars in the Arabidopsis Circadian Clock Michael J. Haydon and Alex A. R. Webb
13. Assessing Protein Stability Under Different Light and Circadian Conditions Takatoshi Kiba and Rossana Henriques
Part IIIAbiotic Stress Responses
14. Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought Diego M. Almeida, M. Cecнlia Almadanim, Tiago Louren
зo, Isabel A. Abreu, Nelson J. M. Saibo, and M. Margarida Oliveira
15. Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis Thaliana Urszula Piskurewicz and Luis Lopez-Molina
16. Assessing Tolerance to Heavy-Metal Stress in Arabidopsis Thaliana Seedings Estelle Remy and Paula Duque
17. Assessing Drought Responses Using Thermals Infrared Imaging Ankush Prashar and Hamlyn G. Jones
18. Generating Targeted Gene Knockout Lines in Physcomotrella Patens to Study Evolution of Stress- Responsive Mechanisms Monika Maronova and Maria Kalyna
19. Screening Stress Tolerance Traits in Arabidopsis Cell Cultures Imma P. Salamу, Bogбta Boros, and L
бszlу Szabados
20. Using Arabidopsis Protoplasts to Study Cellular responses to Environmental Stress Ana Confraria and Elena Baena-Gonzбlez
21. Construction of Artificial miRNAs to Prevent Drought Stress in Solanum Tuberosum Anna Wyrzykowska, Marcin Pieczyński, and Zofia Szweykowska-Kuli
Автор: Chang Paul, Ohi Ryoma Название: The Mitotic Spindle: Methods and Protocols ISBN: 1493980726 ISBN-13(EAN): 9781493980727 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I Methods Focused on Cytology 1: Using Fluorescence Microscopy to Study MitosisSai K. Balchand, Barbara J. Mann, and Patricia Wadsworth 2: Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation Bin He and Daniela Cimini
Part II Methods Focused on Microtubules and the Mitotic Spindle
3: Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg Extracts Aaron C. Groen and Timothy J. Mitchison 4: Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro Marija Zanic
5: Imaging and Quantifying the Dynamics of γ-Tubulin at Microtubule Minus Ends in Mitotic Spindles Nicolas Lecland and Jens Lьders 6: Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy Matthew King and Sabine Petry 7: Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions in Synthetic Cell-Like Compartments with Tunable Size Matthew C. Good Part III Methods Focused on Kinetochores and the Kinetochore-Microtubule Interface 8: In Vitro Kinetochore Assembly Matthew D. D. Miell and Aaron F. Straight 9: Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes Tatsuya Nishino and Tatsuo Fukagawa 10: Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells Keith F. DeLuca, Jacob A. Herman, and Jennifer G. DeLuca 11: Studying Kinetochores In Vivo Using FLIM-FRET Tae Yeon Yoo and Daniel J. Needleman Section IV Methods Focused on the Spindle Pole 12: Purification of Fluorescently Labeled Saccharomyces Cerevisiae Spindle Pole Bodies Kimberly K. Fong, Beth Graczyk, and Trisha N. Davis 13: A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome Conversion Rajesh Kumar Soni and Meng-Fu Bryan Tsou 14: Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation Vladimir Joukov, Johannes C. Walter, and Arcangela De Nicolo Part V Methods Focused on the Cellular Functions of Microtubule Motor Proteins 15: Analyzing Spindle Positioning Dynamics in Cultured Cells Tomomi Kiyomitsu 16: Quantification of Mitotic Chromosome Alignment Cindy Fonseca and Jason Stumpff 17: Imaging Mitosis in the Moss Physcomitrella Patens Moй Yamada, Tomohiro Miki, and Gohta Goshima 18: Small Molecule Approach to Study the Function of Mitotic Kinesins Naowras Al-Obaidi, Johanna Kastl, and Thomas U. Mayer Part VI Novel Approaches to Study Spindle Function and Regulation 19: Identification and Characterization of Mitotic Spindle-Localized Transcripts Amy B. Emerman, Ashwini Jambhekar, and Michael D. Blower 20: Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible Dimerization Edward R. Ballister and Michael A. Lampson 21: Studying Kinetochore Kinases Adrian T. Saurin and Geert J.P.L. Kops 22: Engineering and Functional Analysis of Mitotic Kinases through Chemical Genetics Mathew J.K. Jones and Prasad V. Jallepalli Part VII The Mitotic Spindle and Cancer 23: Using Cell Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer Mijung Kwon
Автор: Benoit Guillemette; Luc R. Gaudreau Название: Histones ISBN: 1493966286 ISBN-13(EAN): 9781493966288 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides a comprehensive list of protocols for molecular biologists, biochemists and geneticists. Chapters cover protocols that further the study into protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the nucleosome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Histones: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Автор: Sarkar Sibaji Название: Histone Deacetylases: Methods and Protocols ISBN: 1493981110 ISBN-13(EAN): 9781493981113 Издательство: Springer Рейтинг: Цена: 6986.00 р. Наличие на складе: Поставка под заказ.
Описание: This volume provides different methodologies for all classes of histone deacetylases, which includes detailed procedures on Class I and II histone deacetylase inhibitors, SIRT inhibitors, and bromodomain inhibitors.
Автор: Shelley L. Berger; O. Nakanishi; Bernhard Haendler Название: The Histone Code and Beyond ISBN: 364242175X ISBN-13(EAN): 9783642421754 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Methylation of DNA at cytosine residues as well as post-translational modifications of histones, including phosphorylation, acetylation, methylation and ubiquitylation, contribute to the epigenetic information carried by chromatin.
Автор: Stefan Jurga; Jan Barciszewski Название: The DNA, RNA, and Histone Methylomes ISBN: 3030147916 ISBN-13(EAN): 9783030147914 Издательство: Springer Рейтинг: Цена: 23757.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book reviews the chemical, regulatory, and physiological mechanisms of protein arginine and lysine methyltransferases, as well as nucleic acid methylations and methylating enzymes. Protein and nucleic acid methylation play key and diverse roles in cellular signalling and regulating macromolecular cell functions. Protein arginine and lysine methyltransferases are the predominant enzymes that catalyse S-adenosylmethionine (SAM)-dependent methylation of protein substrates. These enzymes catalyse a nucleophilic substitution of a methyl group to an arginine or lysine side chain nitrogen (N) atom. Cells also have additional protein methyltransferases, which target other amino acids in peptidyl side chains or N-termini and C-termini, such as glutamate, glutamine, and histidine. All these protein methyltransferases use a similar mechanism. In contrast, nucleic acids (DNA and RNA) are substrates for methylating enzymes, which employ various chemical mechanisms to methylate nucleosides at nitrogen (N), oxygen (O), and carbon (C) atoms. This book illustrates how, thanks to there ability to expand their repertoire of functions to the modified substrates, protein and nucleic acid methylation processes play a key role in cells.
Автор: Jurga Stefan, Barciszewski Jan Название: The Dna, Rna, and Histone Methylomes ISBN: 3030147940 ISBN-13(EAN): 9783030147945 Издательство: Springer Рейтинг: Цена: 23757.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book reviews the chemical, regulatory, and physiological mechanisms of protein arginine and lysine methyltransferases, as well as nucleic acid methylations and methylating enzymes. Protein and nucleic acid methylation play key and diverse roles in cellular signalling and regulating macromolecular cell functions. Protein arginine and lysine methyltransferases are the predominant enzymes that catalyse S-adenosylmethionine (SAM)-dependent methylation of protein substrates. These enzymes catalyse a nucleophilic substitution of a methyl group to an arginine or lysine side chain nitrogen (N) atom. Cells also have additional protein methyltransferases, which target other amino acids in peptidyl side chains or N-termini and C-termini, such as glutamate, glutamine, and histidine. All these protein methyltransferases use a similar mechanism. In contrast, nucleic acids (DNA and RNA) are substrates for methylating enzymes, which employ various chemical mechanisms to methylate nucleosides at nitrogen (N), oxygen (O), and carbon (C) atoms. This book illustrates how, thanks to there ability to expand their repertoire of functions to the modified substrates, protein and nucleic acid methylation processes play a key role in cells.
ООО "Логосфера " Тел:+7(495) 980-12-10 www.logobook.ru
