Автор: Aphasizhev Ruslan Название: RNA and DNA Editing: Methods and Protocols ISBN: 1493958216 ISBN-13(EAN): 9781493958214 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The recent expansion in diversity of RNA and DNA editing types has stimulated the development of many unique genetic, molecular, biochemical, and computational approaches to biological issues. In RNA and DNA Editing: Methods and Protocols, leading experts in the field introduce methods developed over the last few years to study editing substrates, mechanisms of specificity, and functions of RNA and DNA editing enzymes and complexes. Sections of the book are dedicated to state-of-the art techniques which enable investigation of uracil insertion/deletion RNA editing in mitochondrion of Trypanosoma brucei, adenosine to inosine RNA editing, cytidine to uracil RNA and DNA editing, as well as tRNA editing and RNA modifications. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA and DNA Editing: Methods and Protocols seeks to inspire the further development of these vital and powerful techniques.
Автор: Turksen Название: Genome Editing ISBN: 3319341464 ISBN-13(EAN): 9783319341460 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This timely volume explores the use of CRISPR-Cas9 for genome editing, presenting cutting-edge techniques and their applications in treatment of disease. The chapters describe latest methods such as use of targetable nucleases, investigation of the non-coding genome, mouse genome editing, increasing of knock-in efficiency in mouse zygotes, and generation of reporter stem cells; the text contextualizes these methods in treatment of cardiovascular disease, diabetes mellitus, retinitis pigmentosa, and others. The final chapters round out the book with a discussion of controversies and future directions.
Genome Editing is an essential, of-the-moment contribution to this rapidly growing field. Drawing from a wealth of international perspectives, it presents novel techniques and applications for the engineering of the human genome. This book is essential reading for all clinicians and researchers in stem cells, regenerative medicine, genomics, biochemical and biomedical engineering- especially those interested in learning more about genome editing and applying it in a targeted, specific way.
Автор: Toni Cathomen; Matthew Hirsch; Matthew Porteus Название: Genome Editing ISBN: 1493935070 ISBN-13(EAN): 9781493935079 Издательство: Springer Рейтинг: Цена: 19591.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: "American Society of Gene & Cell Therapy"--Cover.
Автор: Izuho Hatada Название: Genome Editing in Animals ISBN: 1493971271 ISBN-13(EAN): 9781493971275 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Construction and Evaluation of Zinc Finger Nucleases
Hiroshi Ochiai and Takashi Yamamoto
2. Current Overview of TALEN Construction Systems
Tetsushi Sakuma and Takashi Yamamoto
3. CRISPR/Cas9
Izuho Hatada and Takuro Horii
4. Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-Target Risks in Human and Mouse
Qingbo Wang and Kumiko Ui-Tei
5. Genome Editing of Mouse by Cytoplasmic Injection
Takuro Horii and Izuho Hatada
6. Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing sgRNA/Cas9 Expressing Plasmids
Taichi Noda, Asami Oji, and Masahito Ikawa
7. Genome Editing in Mouse and Rat by Electroporation
Takehito Kaneko
8. Generation of Knock-in Mouse by Genome Editing
Wataru Fujii
9. Genome Editing of Rat
Takehito Kaneko
10. Gene Targeting in Rabbits: Single-step Generation of Knockout Rabbits by Microinjection of CRISPR/Cas9 Plasmids
Yoshihiro Kawano and Arata Honda
11. Genome Editing of Pig
Masahito Watanabe and Hiroshi Nagashima
12. Genome Editing of Monkey
Zhen Liu, Yijun Cai, and Qiang Sun
13. Genome Editing Mediated by Primordial Germ Cell in Chicken
Jae Yong Han and Hong Jo Lee
14. CRISPR/Cas9-mediated Targeted Knock-in of Exogenous Reporter Genes in Zebrafish
Atsuo Kawahara
15. Genome Editing of Medaka
Satoshi Ansai and Masato Kinoshita
16. A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders using the CRISPR-Cas System
Yuto Sakane, Ken-ich T Suzuki, and Takashi Yamamoto
17. Genome Editing of Silkworms
Takuya Tsubota and Hideki Sezutsu
18. Genome Editing in the Cricket, Gryllus bimaculatus
Takahito Watanabe, Sumihare Noji, and Taro Mito
19. Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease
Yasunori Sasakura, Keita Yoshida, and Nicholas Treen
20. &
Автор: Rudolf Jaenisch; Feng Zhang; Fred Gage Название: Genome Editing in Neurosciences ISBN: 3319601911 ISBN-13(EAN): 9783319601915 Издательство: Springer Рейтинг: Цена: 6986.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book is open access under a CC BY 4.0 license.
CRISPR-Cas9 is a rapid, efficient, versatile and relatively cheap method for dissecting the molecular pathways that are the basis of life, as well as for investigating and potentially rectifying faults in these pathways that result in disease.
This book reviews how CRISPR-Cas9 and other genome editing techniques are advancing our understanding of development and function in the nervous system, uncovering the molecular causes of neurological disorders and providing tools for gene therapy.
Автор: Jeltsch Название: Epigenome Editing ISBN: 1493977733 ISBN-13(EAN): 9781493977734 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field.
Название: Human flourishing in an age of gene editing ISBN: 0190940360 ISBN-13(EAN): 9780190940362 Издательство: Oxford Academ Рейтинг: Цена: 6018.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: International uproar followed the recent announcement of the birth of twin girls whose genomes had been edited with a breakthrough DNA editing-technology. This technology, called clustered regularly interspaced short palindrome repeats or CRISPR-Cas9, can alter any DNA, including DNA in embryos, meaning that changes can be passed to the offspring of the person that embryo becomes. Should we use gene editing technologies to change ourselves, our children, and future generations to come? The potential uses of CRISPR-Cas9 and other gene editing technologies are unprecedented in human history. By using these technologies, we eradicate certain dreadful diseases. Altering human DNA, however, raises enormously difficult questions. Some of these questions are about safety: Can these technologies be deployed without posing an unreasonable risk of physical harm to current and future generations? Can all physical risks be adequately assessed, and responsibly managed? But gene editing technologies also raise other moral questions, which touch on deeply held, personal, cultural, and societal values: Might such technologies redefine what it means to be healthy, or normal, or cherished? Might they undermine relationships between parents and children, or exacerbate the gap between the haves and have-nots? The broadest form of this second kind of question is the focus of this book: What might gene editing--and related technologies--mean for human flourishing? In the new essays collected here, an interdisciplinary group of scholars asks age--old questions about the nature and well-being of humans in the context of a revolutionary new biotechnology--one that has the potential to change the genetic make-up of both existing people and future generations. Welcoming readers who study related issues and those not yet familiar with the formal study of bioethics, the authors of these essays open up a conversation about the ethics of gene editing. It is through this conversation that citizens can influence laws and the distribution of funding for science and medicine, that professional leaders can shape understanding and use of gene editing and related technologies by scientists, patients, and practitioners, and that individuals can make decisions about their own lives and the lives of their families.
Описание: The cutting-edge science that will revolutionise the way we prevent disease
Автор: Albert Jeltsch; Marianne G. Rots Название: Epigenome Editing ISBN: 1493992813 ISBN-13(EAN): 9781493992812 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Нет в наличии.
Описание: This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Epigenome Editing: Methods and Protocols will be of great assistance to people new to the field but also to those already engaged, as epigenetic editing is still a relatively unexplored field with many issues to be resolved.
Автор: Yonglun Luo Название: CRISPR Gene Editing ISBN: 1493991698 ISBN-13(EAN): 9781493991693 Издательство: Springer Рейтинг: Цена: 25155.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field.
Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.
Автор: Hatada Izuho Название: Genome Editing in Animals: Methods and Protocols ISBN: 1493983938 ISBN-13(EAN): 9781493983933 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Construction and Evaluation of Zinc Finger Nucleases
Hiroshi Ochiai and Takashi Yamamoto
2. Current Overview of TALEN Construction Systems
Tetsushi Sakuma and Takashi Yamamoto
3. CRISPR/Cas9
Izuho Hatada and Takuro Horii
4. Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-Target Risks in Human and Mouse
Qingbo Wang and Kumiko Ui-Tei
5. Genome Editing of Mouse by Cytoplasmic Injection
Takuro Horii and Izuho Hatada
6. Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing sgRNA/Cas9 Expressing Plasmids
Taichi Noda, Asami Oji, and Masahito Ikawa
7. Genome Editing in Mouse and Rat by Electroporation
Takehito Kaneko
8. Generation of Knock-in Mouse by Genome Editing
Wataru Fujii
9. Genome Editing of Rat
Takehito Kaneko
10. Gene Targeting in Rabbits: Single-step Generation of Knockout Rabbits by Microinjection of CRISPR/Cas9 Plasmids
Yoshihiro Kawano and Arata Honda
11. Genome Editing of Pig
Masahito Watanabe and Hiroshi Nagashima
12. Genome Editing of Monkey
Zhen Liu, Yijun Cai, and Qiang Sun
13. Genome Editing Mediated by Primordial Germ Cell in Chicken
Jae Yong Han and Hong Jo Lee
14. CRISPR/Cas9-mediated Targeted Knock-in of Exogenous Reporter Genes in Zebrafish
Atsuo Kawahara
15. Genome Editing of Medaka
Satoshi Ansai and Masato Kinoshita
16. A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders using the CRISPR-Cas System
Yuto Sakane, Ken-ich T Suzuki, and Takashi Yamamoto
17. Genome Editing of Silkworms
Takuya Tsubota and Hideki Sezutsu
18. Genome Editing in the Cricket, Gryllus bimaculatus
Takahito Watanabe, Sumihare Noji, and Taro Mito
19. Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease
Yasunori Sasakura, Keita Yoshida, and Nicholas Treen
20. &
Автор: Cathomen Toni, Hirsch Matthew, Porteus Matthew Название: Genome Editing: The Next Step in Gene Therapy ISBN: 1493980610 ISBN-13(EAN): 9781493980611 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This comprehensive volume explores human genetic engineering its pre-clinical and clinical applications, current developments, and as treatment for hereditary diseases. It presents and evaluates the most recent advances in the understanding of mammalian host DNA repair mechanisms, such as double-strand break induced gene targeting and mutagenesis, the development of zinc-finger nucleases, genome editing for neuromuscular diseases, phase integrases, triplex forming oligonucleotides and peptide nucleic acids, aptamer-guided gene targeting, AAV gene editing via DSB repair, engineered nucleases and trinucleotide repeat diseases, and creation of HIV-resistant cells. The expertly authored chapters contextualize current developments within the history of genome editing while also discussing the current and potential safety concerns of this rapidly growing field. Genome Editing: The Next Step in Gene Therapy, the latest volume in the American Society of Gene and Cell Therapy series, deftly illuminates the potential of genetic engineering technology to eradicate today’s deadliest and most prolific diseases. It is ideal reading for clinicians and researchers in genetics and immunology.
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