The Mitotic Exit Network: Methods and Protocols, Monje-Casas Fernando, Queralt Ethel
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Fernando Monje-Casas; Ethelvina Queralt Badia Название: The Mitotic Exit Network ISBN: 149396500X ISBN-13(EAN): 9781493965007 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed book collects the main methodologies used for the analysis of the activity, localization, and regulation of the components of the Mitotic Exit Network (MEN) pathway during mitotic exit in Saccharomyces cerevisiae, as well as for the evaluation of the roles of these proteins in other cellular processes, such as the condensation of the rDNA, the functionality of the mitotic checkpoints, and cytokinesis. Budding yeast serves as an ideal model system for dissecting the mechanisms that regulate cell cycle progression and providing new insights into the molecular basis of cell cycle control and, thus, into the origin of diseases that arise as a consequence of problems during cell division. Therefore, although this volume concentrates on Saccharomyces cerevisiae as a model, it also details the implications that the research about the MEN have on our understanding of the mitotic exit process in higher eukaryotes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, The Mitotic Exit Network: Methods and Protocols will be a valuable reference for cellular and molecular biologists and biochemists as well as for all scientists interested in the study of the regulation of mitotic exit using budding yeast as a model organism.
Автор: Chang Paul, Ohi Ryoma Название: The Mitotic Spindle: Methods and Protocols ISBN: 1493980726 ISBN-13(EAN): 9781493980727 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I Methods Focused on Cytology 1: Using Fluorescence Microscopy to Study MitosisSai K. Balchand, Barbara J. Mann, and Patricia Wadsworth 2: Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation Bin He and Daniela Cimini
Part II Methods Focused on Microtubules and the Mitotic Spindle
3: Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg Extracts Aaron C. Groen and Timothy J. Mitchison 4: Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro Marija Zanic
5: Imaging and Quantifying the Dynamics of γ-Tubulin at Microtubule Minus Ends in Mitotic Spindles Nicolas Lecland and Jens Lьders 6: Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy Matthew King and Sabine Petry 7: Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions in Synthetic Cell-Like Compartments with Tunable Size Matthew C. Good Part III Methods Focused on Kinetochores and the Kinetochore-Microtubule Interface 8: In Vitro Kinetochore Assembly Matthew D. D. Miell and Aaron F. Straight 9: Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes Tatsuya Nishino and Tatsuo Fukagawa 10: Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells Keith F. DeLuca, Jacob A. Herman, and Jennifer G. DeLuca 11: Studying Kinetochores In Vivo Using FLIM-FRET Tae Yeon Yoo and Daniel J. Needleman Section IV Methods Focused on the Spindle Pole 12: Purification of Fluorescently Labeled Saccharomyces Cerevisiae Spindle Pole Bodies Kimberly K. Fong, Beth Graczyk, and Trisha N. Davis 13: A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome Conversion Rajesh Kumar Soni and Meng-Fu Bryan Tsou 14: Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation Vladimir Joukov, Johannes C. Walter, and Arcangela De Nicolo Part V Methods Focused on the Cellular Functions of Microtubule Motor Proteins 15: Analyzing Spindle Positioning Dynamics in Cultured Cells Tomomi Kiyomitsu 16: Quantification of Mitotic Chromosome Alignment Cindy Fonseca and Jason Stumpff 17: Imaging Mitosis in the Moss Physcomitrella Patens Moй Yamada, Tomohiro Miki, and Gohta Goshima 18: Small Molecule Approach to Study the Function of Mitotic Kinesins Naowras Al-Obaidi, Johanna Kastl, and Thomas U. Mayer Part VI Novel Approaches to Study Spindle Function and Regulation 19: Identification and Characterization of Mitotic Spindle-Localized Transcripts Amy B. Emerman, Ashwini Jambhekar, and Michael D. Blower 20: Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible Dimerization Edward R. Ballister and Michael A. Lampson 21: Studying Kinetochore Kinases Adrian T. Saurin and Geert J.P.L. Kops 22: Engineering and Functional Analysis of Mitotic Kinases through Chemical Genetics Mathew J.K. Jones and Prasad V. Jallepalli Part VII The Mitotic Spindle and Cancer 23: Using Cell Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer Mijung Kwon
Автор: Paul Chang; Ryoma Ohi Название: The Mitotic Spindle ISBN: 1493935402 ISBN-13(EAN): 9781493935406 Издательство: Springer Рейтинг: Цена: 22203.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Part I Methods Focused on Cytology 1: Using Fluorescence Microscopy to Study MitosisSai K. Balchand, Barbara J. Mann, and Patricia Wadsworth 2: Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation Bin He and Daniela Cimini
Part II Methods Focused on Microtubules and the Mitotic Spindle
3: Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg Extracts Aaron C. Groen and Timothy J. Mitchison 4: Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro Marija Zanic
5: Imaging and Quantifying the Dynamics of γ-Tubulin at Microtubule Minus Ends in Mitotic Spindles Nicolas Lecland and Jens Lьders 6: Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy Matthew King and Sabine Petry 7: Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions in Synthetic Cell-Like Compartments with Tunable Size Matthew C. Good Part III Methods Focused on Kinetochores and the Kinetochore-Microtubule Interface 8: In Vitro Kinetochore Assembly Matthew D. D. Miell and Aaron F. Straight 9: Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes Tatsuya Nishino and Tatsuo Fukagawa 10: Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells Keith F. DeLuca, Jacob A. Herman, and Jennifer G. DeLuca 11: Studying Kinetochores In Vivo Using FLIM-FRET Tae Yeon Yoo and Daniel J. Needleman Section IV Methods Focused on the Spindle Pole 12: Purification of Fluorescently Labeled Saccharomyces Cerevisiae Spindle Pole Bodies Kimberly K. Fong, Beth Graczyk, and Trisha N. Davis 13: A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome Conversion Rajesh Kumar Soni and Meng-Fu Bryan Tsou 14: Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation Vladimir Joukov, Johannes C. Walter, and Arcangela De Nicolo Part V Methods Focused on the Cellular Functions of Microtubule Motor Proteins 15: Analyzing Spindle Positioning Dynamics in Cultured Cells Tomomi Kiyomitsu 16: Quantification of Mitotic Chromosome Alignment Cindy Fonseca and Jason Stumpff 17: Imaging Mitosis in the Moss Physcomitrella Patens Moй Yamada, Tomohiro Miki, and Gohta Goshima 18: Small Molecule Approach to Study the Function of Mitotic Kinesins Naowras Al-Obaidi, Johanna Kastl, and Thomas U. Mayer Part VI Novel Approaches to Study Spindle Function and Regulation 19: Identification and Characterization of Mitotic Spindle-Localized Transcripts Amy B. Emerman, Ashwini Jambhekar, and Michael D. Blower 20: Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible Dimerization Edward R. Ballister and Michael A. Lampson 21: Studying Kinetochore Kinases Adrian T. Saurin and Geert J.P.L. Kops 22: Engineering and Functional Analysis of Mitotic Kinases through Chemical Genetics Mathew J.K. Jones and Prasad V. Jallepalli Part VII The Mitotic Spindle and Cancer 23: Using Cell Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer Mijung Kwon
Автор: Vicente-Manzanares Miguel Название: The Integrin Interactome: Methods and Protocols ISBN: 1071609610 ISBN-13(EAN): 9781071609613 Издательство: Springer Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.
Автор: Duque Paula Название: Environmental Responses in Plants: Methods and Protocols ISBN: 1493980297 ISBN-13(EAN): 9781493980291 Издательство: Springer Рейтинг: Цена: 16070.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
PaPart I Tropisms
1.11. Hydrotropism: Analysis of the Root Response to a Moisture Gradient Regina Antoni, Daniela Dietrich, Malcolm J. Bennett, and Pedro L. Rodriguez
2. Assessing Gravitropic Responses in Arabidopsis Richard Barker, Benjamin Cox, Logan Silber, Arah Sangari, Amir Assadi, and Patrick Masson
3. Physiological Analysis of Phototropic Responses in Arabidopsis Mathias Zeidler
4. Automatic Chloroplast Movement Analysis Henrik Johansson and Mathias Zeidler
5. Micro
scopic and Biochemical Visualization of Auxins in Plant Tissues Joshua J. Blakeslee and Angus S. Murphy
6. Immunolocalization of PIN and ABCB Transporters in Plants Nicola Carraro and Wendy A. Peer
Part II Photoperiodism and Circasian Rhythms
7.Analysis of Circadian Leaf Movements Niels A. Mьller and Josй M. Jimйmez-Gуmez
8. Sample Preparation of Arabidopsis Thaliana Shoot Apices for Expression Studies of Photoperiod-Induced Genes Fernando Andrйs, Stefano Torti, Coral Vincent, and George Coupland
9. A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs are
Temporally Restricted by the Circadian Clock Amaury de Montaigu, Markus C. Berns, and Geroge Coupland
10. Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hyrbid Screens Using a Transcription Factor ORFeome Ghislain Breton, Steve A. Kay, and Josй L. Pruneda-Paz
11. Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes Craig G. Simpson, John Fuller, Cristiane P. G. Calixto, Jim McNicol, Clare Booth, John W. S. Brown, and Dorothee Staiger
12. Assessing the Impact of Photosynthetic Sugars in the Arabidopsis Circadian Clock Michael J. Haydon and Alex A. R. Webb
13. Assessing Protein Stability Under Different Light and Circadian Conditions Takatoshi Kiba and Rossana Henriques
Part IIIAbiotic Stress Responses
14. Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought Diego M. Almeida, M. Cecнlia Almadanim, Tiago Louren
зo, Isabel A. Abreu, Nelson J. M. Saibo, and M. Margarida Oliveira
15. Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis Thaliana Urszula Piskurewicz and Luis Lopez-Molina
16. Assessing Tolerance to Heavy-Metal Stress in Arabidopsis Thaliana Seedings Estelle Remy and Paula Duque
17. Assessing Drought Responses Using Thermals Infrared Imaging Ankush Prashar and Hamlyn G. Jones
18. Generating Targeted Gene Knockout Lines in Physcomotrella Patens to Study Evolution of Stress- Responsive Mechanisms Monika Maronova and Maria Kalyna
19. Screening Stress Tolerance Traits in Arabidopsis Cell Cultures Imma P. Salamу, Bogбta Boros, and L
бszlу Szabados
20. Using Arabidopsis Protoplasts to Study Cellular responses to Environmental Stress Ana Confraria and Elena Baena-Gonzбlez
21. Construction of Artificial miRNAs to Prevent Drought Stress in Solanum Tuberosum Anna Wyrzykowska, Marcin Pieczyński, and Zofia Szweykowska-Kuli
Автор: Goswami Ritobrata Название: Th9 Cells: Methods and Protocols ISBN: 1493983261 ISBN-13(EAN): 9781493983261 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The techniques described in Th9 Cells: Methods and Protocols study the role of Th9 cells in different inflammatory disease models, including allergic inflammation model, parasite model, tumor model, and EAE and IBD model.
Автор: Ivan A. Vorobjev Название: Cellular Heterogeneity Methods and Protocols ISBN: 1493976796 ISBN-13(EAN): 9781493976799 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts.
Автор: Blouin Cedric M. Название: Caveolae: Methods and Protocols ISBN: 1071607316 ISBN-13(EAN): 9781071607312 Издательство: Springer Цена: 22359.00 р. Наличие на складе: Поставка под заказ.
Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.
