Nanopore Technology: Methods and Protocols, Fahie Monifa A. V.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Kwok Pui-Yan Название: Single Nucleotide Polymorphisms / Methods and Protocols ISBN: 0896039684 ISBN-13(EAN): 9780896039681 Издательство: Springer Цена: 5809.00 р. Наличие на складе: Есть (2 шт.) Описание: Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
Автор: Branton Daniel, Deamer David Название: Nanopore Sequencing: An Introduction ISBN: 9813270608 ISBN-13(EAN): 9789813270602 Издательство: Marston Book Services Рейтинг: Цена: от 4792.00 р. Наличие на складе: Есть
Описание:
This is an introductory text and laboratory manual to be used primarily in undergraduate courses. It is also useful for graduate students and research scientists who require an introduction to the theory and methods of nanopore sequencing. The book has clear explanations of the principles of this emerging technology, together with instructional material written by experts that describes how to use a MinION nanopore instrument for sequencing in research or the classroom.
At Harvard University the book serves as a textbook and lab manual for a university laboratory course designed to intensify the intellectual experience of incoming undergraduates while exploring biology as a field of concentration. Nanopore sequencing is an ideal topic as a path to encourage students about the range of courses they will take in Biology by pre-emptively addressing the complaint about having to take a course in Physics or Maths while majoring in Biology. The book addresses this complaint by concretely demonstrating the range of topics -- from electricity to biochemistry, protein structure, molecular engineering, and informatics -- that a student will have to master in subsequent courses if he or she is to become a scientist who truly understands what his or her biology instrument is measuring when investigating biological phenomena.
Автор: Daniel Branton, David Deamer Название: Nanopore Sequencing: An Introduction ISBN: 9811213089 ISBN-13(EAN): 9789811213083 Издательство: World Scientific Publishing Рейтинг: Цена: 8712.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
This is an introductory text and laboratory manual to be used primarily in undergraduate courses. It is also useful for graduate students and research scientists who require an introduction to the theory and methods of nanopore sequencing. The book has clear explanations of the principles of this emerging technology, together with instructional material written by experts that describes how to use a MinION nanopore instrument for sequencing in research or the classroom.
At Harvard University the book serves as a textbook and lab manual for a university laboratory course designed to intensify the intellectual experience of incoming undergraduates while exploring biology as a field of concentration. Nanopore sequencing is an ideal topic as a path to encourage students about the range of courses they will take in Biology by pre-emptively addressing the complaint about having to take a course in Physics or Maths while majoring in Biology. The book addresses this complaint by concretely demonstrating the range of topics -- from electricity to biochemistry, protein structure, molecular engineering, and informatics -- that a student will have to master in subsequent courses if he or she is to become a scientist who truly understands what his or her biology instrument is measuring when investigating biological phenomena.
Автор: Maria E. Gracheva Название: Nanopore-Based Technology ISBN: 1493958097 ISBN-13(EAN): 9781493958092 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Research into nanopores holds out the promise of ultrafast-and cheaper-DNA sequencing. This study sets out the molecule characterization techniques now available in the field, with numerous step-by-step protocols and expert troubleshooting advice.
Автор: Joshua B. Edel Название: Engineered Nanopores for Bioanalytical Applications, ISBN: 1437734731 ISBN-13(EAN): 9781437734737 Издательство: Elsevier Science Рейтинг: Цена: 17348.00 р. Наличие на складе: Поставка под заказ.
Описание: Focuses on practical analytical applications of nanopore development. This book provides a professional reference on nanopores for readers in academia, industry and engineering and biomedical fields. It describes the instrumentation, fabrication, and experimental methods necessary to carry out nanopore-based experiments for developing new devices.
Автор: Samir M. Iqbal; Rashid Bashir Название: Nanopores ISBN: 1489988254 ISBN-13(EAN): 9781489988256 Издательство: Springer Рейтинг: Цена: 20896.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book examines the emerging research directions surrounding nanopores such as genome sequencing and early disease detection using biomarker identification. It covers the applications of nanopores in genetics, proteomics, drug discovery and more.
Автор: Theodoridis Georgios A., Gika Helen G., Wilson Ian D. Название: Metabolic Profiling: Methods and Protocols ISBN: 1493976427 ISBN-13(EAN): 9781493976423 Издательство: Springer Рейтинг: Цена: 13275.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Helen G. Gika, Georgios A. Theodoridis, and Ian D. Wilson
2. Quality Control and Validation Issues in LC-MS Metabolomics
Olga Begou, Helen G. Gika, Georgios A. Theodoridis, and Ian D. Wilson
3. Data Treatment for LC-MS Untargeted Analysis
Samantha Riccadonna and Pietro Franceschi
4. Bio- and Chemoinformatics Approaches for Metabolomics Data Analysis
Michael Witting
Part II Methods
5. HILIC- MS/MS Multi-Targeted Method for Metabolomics Applications
Christina Virgiliou, Helen G. Gika, and Georgios A. Theodoridis
6. Ion Pair Chromatography for Endogenous Metabolites LC-MS Analysis in Tissue Samples Following Targeted Acquisition
Filippos Michopoulos
7. LC-MS Untargeted Analysis
Elizabeth Want
8. NMR Based Metabolic Profiling Procedures for Biofluids, and Cell and Tissue Extracts
Dimitra Benaki and Emmanuel Mikros
9. Untargeted GC-MS Metabolomics
Μatthaios-Εmmanouil P. Papadimitropoulos, Catherine G. Vasilopoulou, Christoniki Maga-Nteve, and Maria I. Klapa
10. Rat Fecal Metabolomics Based Analysis
Olga Deda, Helen G. Gika, and Georgios A. Theodoridis
11. GC-MS Metabolomic Profiling of Protic Metabolites Following Heptafluorobutyl Chloroformate Mediated Dispersive Liquid Microextraction Sample Preparation Protocol
Petr Husek, Zdeněk Svagera, Dagmar Hanzlнkovб, Iva Opekarovб, Lucie Řimnбčovб, Helena Zahradnнčkovб, and Petr Simek
12. Sheathless Capillary Electrophoresis-Mass Spectrometry for the Profiling of Charged Metabolites in Biological Samples
Rawi Ramautar
Part III Plant/Food and Other Applications
13. Two-Phase Extraction for Comprehensive Analysis of the Plant Metabolome by NMR
Jan Schripsema and Denise Dagnino
14. NMR Spectroscopy Protocols for Food Metabolomics Applications
Evangelia Ralli, Maria Amargianitaki, Efi Manolopoulou, Maria Misiak, Georgios Markakis, Sofia Tachtalidou, Alexandra Kolesnikova, Photis Dais, and Apostolos Spyros
15. Direct Injection Analysis of Fruit VOCs by PTR-ToF-MS: The Apple Case Study
Brian Farneti
16. LC-MS Untargeted Protocol for the Analysis of Wine
Panagiotis Arapitsas and Fulvio Mattivi
Part IV Life Science Applications
17. Tissue Multiplatform-Based Metabolomics/Metabonomics for Enhanced Metabolome Coverage
Panagiotis A. Vorkas, M.R. Abellona U, and Jia V. Li
18. UHPLC-HRMS Analysis for Steroid Profiling in Serum (Steroidomics)
19. Metabolomics in Human Acute-Exercise Trials: Study Design and Preparation
Aikaterina Siopi and Vassilis Mougios
Автор: Peter Molnar; James J. Hickman Название: Patch-Clamp Methods and Protocols ISBN: 1617377244 ISBN-13(EAN): 9781617377242 Издательство: Springer Рейтинг: Цена: 21661.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively.
Автор: Patel Tushar Название: Extracellular RNA: Methods and Protocols ISBN: 1493976516 ISBN-13(EAN): 9781493976515 Издательство: Springer Рейтинг: Цена: 11179.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Extracellular RNAs - A New Awareness of Old Perspectives
Noah Sadik, Lilian Cruz, Alessandra Gurtner, Rodosthenis S. Rodosthenous, Sophie A. Dusoswa, Olivia Ziegler, Thomas Sebastiaan Van Solinge, Zhiyun Wei, Ane Miren Salvador Garicano, Bence Gyorgy, Marike Broekman, and Leonora Balaj
2. Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles
Julia Small, Roger Alexander, and Leonora Balaj
3. Extracellular RNA Isolation from Cell Culture Supernatant
Sawen Bakr., Bridget Simonson, Kirsty M. Danielson, and Saumya Das
4. Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture
Irene K. Yan, Neha Shukla, David A. Borrelli, and Tushar Patel
5. Isolation of Extracellular RNA from Serum/Plasma
Justyna Filant, Parham Nejad, Anu Paul, Bridget Simonson, Srimeenakshi Srinivasan, Xuan Zhang, Leonora Balaj, Saumya Das, Roopali Gandhi, Louise C. Laurent, and Anil K. Sood
6. Isolation of Extracellular RNA from Bile
Irene K. Yan, Valentin X. Berdah, and Tushar Patel
7. Cushioned-Density Gradient Ultracentrifugation (C-DGUC): a Refined and High Performance Method for the Isolation, Characterization & Use of Exosomes
Kang Li, David K. Wong, King Yeung Hong, and Robert L. Raffai
8. Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids
Pete Heinzelman
9. Enrichment of Extracellular Vesicle Subpopulations via Affinity Chromatography
Michelle E. Hung, Stephen B. Lenzini, Devin M. Stranford, and Joshua N. Leonard
10. Detection and Analysis of Non-vesicular Extracellular RNA
Juan Pablo Tosar and Alfonso Cayota
11. Isolation of Plasma Lipoproteins as a Source of Extracellular RNA
Kang Li, David K. Wong, Fu Sang Luk, Roy Y. Kim, and Robert L. Raffai
12. Droplet Digital PCR for Quantitation of Extracellular RNA
Irene K Yan, Rishabh Lohray, and Tushar Patel
13. Preparation of Small RNA NGS Libraries from Biofluids
Alton Etheridge, Kai Wang, David Baxter, and David Galas
14. Multiplexed Detection and Quantitation of Extracellular Vesicle RNA Expression using NanoString
Neha Shukla, Irene K. Yan, and Tushar Patel
15. Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs
Akiko Matsuda and Tushar Patel
16. Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs
Marie-Cйcile Didiot, Reka A. Haraszti, Neil Aronin, and Anastasia Khvorova
Автор: Pamela J. Russell; Paul Jackson; Elizabeth A. King Название: Prostate Cancer Methods and Protocols ISBN: 1489938508 ISBN-13(EAN): 9781489938503 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
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