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Stem Cells and Tissue Repair: Methods and Protocols, Kioussi Chrissa


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Автор: Kioussi Chrissa
Название:  Stem Cells and Tissue Repair: Methods and Protocols
ISBN: 9781071606544
Издательство: Springer
Классификация:

ISBN-10: 1071606549
Обложка/Формат: Hardcover
Страницы: 214
Вес: 0.59 кг.
Дата издания: 31.05.2020
Серия: Methods in molecular biology
Язык: English
Издание: 2nd ed. 2020
Иллюстрации: 33 tables, color; 34 illustrations, color; 2 illustrations, black and white; xi, 214 p. 36 illus., 34 illus. in color.; 33 tables, color; 34 illustrat
Размер: 25.91 x 19.56 x 1.52 cm
Читательская аудитория: Professional & vocational
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: This volume looks at a collection of stem cell and regenerative techniques used by both novice and expert scientists. Chapters cover topics such as tissue repaired by expansion and reprogramming; induced pluripotent stem cells driven in neuronal or vascular differentiation; using mesenchymal stem cells to derive skeletal muscle, osteoblasts, and spermatogonial cells; and the technique of monitoring the development of sub-organ microenvironments in the developing pancreas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Stem Cells and Tissue Repair: Methods and Protocols, Second Edition is a valuable resource that provides readers with the latest descriptions and references for exploring this vast field in regenerative medicine.
Дополнительное описание: Culturing and Manipulating Mouse Embryonic Stem Cells.- Generation of an Induced Pluripotent Stem Cell Line with the Constitutive EGFP Reporter.- Engraftable Induced Pluripotent Stem Cell-Derived Neural Precursors for Brain Repair.- In Vitro Direct Reprog



Affinity Chromatography / Methods and Protocols

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
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Цена: 2000.00 р.
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Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

In Situ Detection of DNA Damage / Methods and Protocols

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
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Цена: 5809.00 р.
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Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Adult Stem Cells: Methods and Protocols

Автор: Di Nardo Paolo, Dhingra Sanjiv, Singla Dinender K.
Название: Adult Stem Cells: Methods and Protocols
ISBN: 1493982931 ISBN-13(EAN): 9781493982936
Издательство: Springer
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Цена: 16769.00 р.
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Описание:

Preface...
Table of Contents...
Contributing Authors...

1. A Simple Protocol to Isolate, Characterize and Expand Dental Pulp Stem Cells
Federica Di Scipio, Andrea Elio Sprio, Maria Elisabetta Carere, Zhiqian Yang, and Giovanni Nicolao Berta

2. Derivation of Mesenchymal Stem cells from Embryonic Stem Cells: A Non-Variable and Inexhaustive Source of Adult Stem Cells
Glen Lester Sequiera, Niketa Sareen, Ejlal Abu Al-Rub, and Sanjiv Dhingra

3. Compact Bone-Derived Multipotent Mesenchymal Stromal Cells (MSCs) for the Treatment of Sjogren's-Like Disease in NOD Mice
Ghada Abu Elghanam, Younan Liu, Saeed Khalili, Dongdong Fang, and Simon D.Tran

4. Manipulating the Proliferative Potential of Cardiomyocytes by Gene Transfer
Giulia Prosdocimo and Mauro Giacca

5. Assessment of Energy Metabolic Changes in Adipose Tissue-Derived Stem Cells
Ghazaleh Hajmousa and Martin C. Harmsen

6. Isolation and In Vitro Characterization of Epidermal Stem Cells
Kasper S. Moestrup, Marianne S. Andersen, and Kim B. Jensen

7. Endothelial Progenitor Cells: Procedure for Cell Isolation and Characterization
Garikipati V.N. Srikanth, Zhongjian Cheng, and Raj Kishore

8. Therapeutic Application of Placental Mesenchymal Stem Cells Reprogrammed Neurospheres in Spinal Cord Injury of SCID
Vikram Sabapathy, Franklin Jebaraj Herbert, and Sanjay Kumar

9. Therapeutic Application of Human Wharton Jelly Mesenchymal Stem Cells in Skin Injury of SCID
Vikram Sabapathy, Balasubramanian Sundaram, and Sanjay Kumar

10. Isolation, Characterization, and Expansion of Cancer Stem Cells
Luke A. Torre-Healy, Artem Berezovsky, and Justin D. Lathia

11. Cardiac Progenitor Cell Isolation from Human Auricles
Paolo Di Nardo and Francesca Pagliari

12. Isolation and Culture of Satellite Cells from Mouse Skeletal Muscle
Antonio Musarт and Silvia Carosio

13. Isolation of Stromal Stem Cells from Adipose Tissue
Maria Prat, Francesca Oltolina, Silvia Antonini, and Andrea Zamperone

14. Human Induced Pluripotent Stem Cell-Derived Mesencymal Stem Cells as an Individual-Specific and Renewable Source of Adult Stem Cells15. A Simplified and Systematic Method to Isolate, Culture and Characterize Multiple Types of Human Dental Stem Cells from a Single Tooth
Mohammed Bakkar, Younan Liu , Dongdong Fang, Camille Stegen, Xinyun Su, Murali Ramamoorthi, Li-Chieh Lin, Takako Kawasaki, Nicholas Makhoul, Huan Pham, Yoshinori Sumita, and Simon D. Tran

16. Measurement of Autophagy by Flow Cytometry
Silvia Zappavigna, Angela Lombardi, Gabriella Misso, Anna Grimaldi, and Michele Caraglia

17. Echocardiography-Guided Intramyocardial Injection Method in a Murine Model
Kay Maeda, Rick Seymour, Marc Ruel, and Erik J. Suuronen

18. Noninvasive Assessment of Cell Fate and Biology in Transplanted Mesenchymal Stem Cells
Federico Franchi and Martin Rodriguez-Porcel

19. Methods for Long Term Storage of Murine Bone Marrow Derived Mesenchymal Stem Cells
Niketa Sareen, Ejlal Abu-El-Rub, Glen Lester Sequiera, Meenal Moudgil, and Sanjiv Dhingra

20. Therapeutic Application of Adult Stem Cells in the Heart
Taylor A. Johnson and Dinender K. Singla

21. Patient-Derived and Intra-Operatively Formed Biomaterial for Tissue Engineering
Shalmli U. Joshi, Rares O. Barbu, Melissa Carr-Reynolds, Brian Barnes, and Syam P. Nukavarapu

22. Three-Dimensional Printed Scaffolds with Multipotent Mesenchymal Stromal Cells for Rabbit Mandibular Reconstruction and Engineering
Dongdong Fang, Michael Roskies, Mohamed-Nur Abdallah, Mohammed Bakkar, Jack Jordan, Li-Chieh Lin, Faleh Tamimi, and Simon D. Tran

23. Optimal Enviro

Muscle Stem Cells: Methods and Protocols

Автор: Perdiguero Eusebio, Cornelison Ddw
Название: Muscle Stem Cells: Methods and Protocols
ISBN: 1493982966 ISBN-13(EAN): 9781493982967
Издательство: Springer
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Цена: 15372.00 р.
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Описание: This volume provides leading-edge protocols in the study of the molecular and cellular biology of muscle stem cells.

Leukemia Stem Cells: Methods and Protocols

Автор: Cobaleda Cйsar, Sбnchez-Garcнa Isidro
Название: Leukemia Stem Cells: Methods and Protocols
ISBN: 1071608096 ISBN-13(EAN): 9781071608098
Издательство: Springer
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Цена: 18167.00 р.
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Описание: The detailed volume aims to provide a comprehensive hands-on manual covering all the techniques involved in the cellular and molecular identification and characterization of both normal hematopoietic and leukemic stem cells, both from human patients and from mouse models of human leukemia.

Rnai and Small Regulatory Rnas in Stem Cells: Methods and Protocols

Автор: Zhang Baohong
Название: Rnai and Small Regulatory Rnas in Stem Cells: Methods and Protocols
ISBN: 1493983865 ISBN-13(EAN): 9781493983865
Издательство: Springer
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Цена: 16070.00 р.
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Описание:

Part I: Introduction

1. RNAi Technique in Stem Cell Research: Current Status and Future Perspectives

Gang-Ming Zou

2. RNAi and MicroRNA-Mediated Gene Regulation in Stem Cells

Alexander K. Murashov

Part II: RNA Interference

3. Forward RNAi Screens in Human Hematopoietic Stem Cells

Roman Galeev, Christine Karlsson, Aurйlie Baudet, and Jonas Larsson

4. Silencing of ATP11B by RNAi-Induced Changes in Neural Stem Cell Morphology

Jiao Wang, Qian Wang, Fangfang Zhou, Dong Wang, and Tieqiao Wen

5. High-Efficiency Transfection of Glioblastoma Cells and a Simple Spheroid Migration Assay

Carsten Hagemann, Diana Amend, Almuth F. Kessler, Thomas Linsenmann, Ralf-Ingo Ernestus, and Mario Lцhr

6. Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells

Xiaoxing Cheng and Rui Jian

7. Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cells

Soizik Berlivet, Isabelle Hmitou, Hйlиne Picaud, and Matthieu Gйrard

8. Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8

Takayuki Manabe and Akio Wanaka

9. A Recessive Genetic Screen for Components of the RNA Interference Pathway Performed in Mouse Embryonic Stem Cells

Melanie I. Trombly and Xiaozhong Wang

10. RNAi Knockdown of Ape1 Gene in the Differentiation of Mouse Embryonic Stem Cells

Gang-Ming Zou, Jieqing Yu, Cynthia LeBron, and Yumei Fu

11. An Efficient Transfection Method for Differentiation and Cell Proliferation of Mouse Embryonic Stem Cells

Jun-Yang Liou, Bor-Sheng Ko, and Tzu-Ching Chang

12. Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetry

Mario Lцhr, Thomas Linsenmann, Anna Jawork, Almuth F. Kessler, Nils Timmermann, Gyцrgy A. Homola, Ralf-Ingo Ernestus, and Carsten Hagemann

13. RNAi-Based Techniques for the Analysis of Gene Function in Drosophila Germline Stem Cells

Amelia J. Helms, Danielle S. Finger, Victoria L. Hardy, and Elizabeth T. Ables

14. In Vivo RNAi in the Drosophila Follicular Epithelium: Analysis of Stem Cell Maintenance, Proliferation, and Differentiation

Veit Riechmann

15. A Phenotype-Based RNAi Screening for Ras-ERK/MAPK Signaling-Associated Stem Cell Regulators in C. elegans

Myon-Hee Lee and Dong Suk Yoon

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DNA Repair Protocols: Eukaryotic Systems (Methods in Molecular Biology)

Автор: Daryl S. Henderson
Название: DNA Repair Protocols: Eukaryotic Systems (Methods in Molecular Biology)
ISBN: 1468471449 ISBN-13(EAN): 9781468471441
Издательство: Springer
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Цена: 22203.00 р.
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Stem Cells and Tissue Repair

Автор: Chrissa Kioussi
Название: Stem Cells and Tissue Repair
ISBN: 1493955322 ISBN-13(EAN): 9781493955329
Издательство: Springer
Рейтинг:
Цена: 13974.00 р.
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Описание:

1. Culturing and Differentiating Mouse Embryonic Stem Cells

Axel P. Gross and Chrissa Kioussi

2. Neural Stem Cells Transplantation in an Animal Model of Traumatic Brain Injury

Dimitra Thomaidou

3. Experimental Cell Transplantation for Traumatic Spinal Cord Injury Regeneration: Intramedullar or Intrathecal Administration

Ana Alastrue-Agudo, Slaven Erceg, Marta Cases-Villar, Viviana Bisbal-Velasco, Richard J. Griffeth, Francisco Javier Rodriguez-Jimйnez, and Victoria Moreno-Manzano

4. Generation of Murine Xenograft Models of Brain Tumors from Primary Human Tissue for in vivo Analysis of the Brain Tumor Initiating Cell

Maleeha Qazi, Aneet Mann, Randy van Ommeren, Chitra Venugopal, Nicole McFarlane, Parvez Vora, and Sheila K. Singh

5. Growth of Bone Marrow and Skeletal Muscle Side Population Stem Cells in Suspension Culture

Christina A. Pacak and Douglas B. Cowan

6. Isolation, Culture and Immunostaining of Skeletal Muscle Fibers to Study Myogenic Progression in Satellite Cells

Louise A. Moyle and Peter S. Zammit

7. Human Neural Crest Stem Cells Derived from Human Pluripotent Stem Cells

Qiuyue Liu, Andrzej Swistowski, and Xianmin Zeng

8. Dental Pulp Stem Cells (DPSC) Isolation, Characterization and Differentiation

Federico Ferro, Renza Spelat, and Chelsea S. Baheney

9. Dental Pulp Stem Cells Isolation and Osteogenic Differentiation: A Good Promise for Tissue Engineering

Adriana Di Benedetto, Claudia Carbone, and Giorgio Mori

10. Efficient Hepatic Differentiation of Human Induced Pluripotent Stem Cells in A Three-Dimensional Microscale Culture

Ran-Ran Zhang, Takanori Takebe, Leina Miyazaki, Maho Takayama, Hiroyuki Koike, Masaki Kimura, Masahirop Enomura, Yun-Wen Zheng, Keisuke Sekine, and Hideki Taniguchi

11. The Generation and Maintenance of Rat Induced Pluripotent Stem Cells

Tomoyuki Yamaguchi, Sanae Hamanaka, and Hiromitsu Nakauchi

12. Protocol for Cutaneous Wound Healing Assay in a Murine Model

Gitali Ganguli-Indra

13. Adipose Derived Stem Cells: Methods for Isolation and Applications for Clinical Use

Brian Mailey, Ava Hosseini, Jennifer Baker, Adam Young, Zeni Alfonso, Kevin Hicok, Anne M. Wallace, and Steven Cohen

14. In vitro Detection of Residual Undifferentiated Cells in Retinal Pigment Epithelial Cells Derived from Human Induced Pluripotent Stem Cells

Takuya Kuroda, Satoshi Yasuda, and Yoji Sato

15. Whole Mount Immunohistochemistry to Study Spermatological Stem Cells and Spermatogenic Lineage in Mice, Monkeys and Humans

Kathrin Gassei, Hanna Valli, and Kyle E. Orwig

16. Differentiating the Stem Cell Pool of Human Hair Follicle Outer Root Sheath into Functional Melanocytes

Marie Schneider, Christina Dieckmann, Kathrin Rabe, Jan-Christoph Simon, and Vuk Savkovic

17. Pancreas Development ex vivo: Culturing Embryonic Pancreas Explants on Permeable Culture Inserts, with Firbonectin-Coated Glass Microwells, or Embedded in Three-Dimensional MatrigelTM

Hung Ping Shih and Maike Sander

18. Ultra-Rapid Manufacturing of Engineered Epicardial Substitute to Regenerate Cardiac Tissue Following Acute Ischemic Injury

Vahid Serpooshan and Pilar Ruiz-Lozano

Th9 Cells: Methods and Protocols

Автор: Goswami Ritobrata
Название: Th9 Cells: Methods and Protocols
ISBN: 1493983261 ISBN-13(EAN): 9781493983261
Издательство: Springer
Рейтинг:
Цена: 19564.00 р.
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Описание: The techniques described in Th9 Cells: Methods and Protocols study the role of Th9 cells in different inflammatory disease models, including allergic inflammation model, parasite model, tumor model, and EAE and IBD model.

Innate Lymphoid Cells: Methods and Protocols

Автор: Amarnath Shoba
Название: Innate Lymphoid Cells: Methods and Protocols
ISBN: 107160337X ISBN-13(EAN): 9781071603376
Издательство: Springer
Рейтинг:
Цена: 15372.00 р.
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Epidermal Cells: Methods and Protocols

Автор: Turksen Kursad
Название: Epidermal Cells: Methods and Protocols
ISBN: 1071602500 ISBN-13(EAN): 9781071602508
Издательство: Springer
Рейтинг:
Цена: 18167.00 р.
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Описание: This fully updated edition brings together a new set of protocols to arm epidermal cell biologists with tools and approaches to continue the quest to uncover the intricacies and regulatory mechanisms underlying epidermal cell development and function.

Stem Cell Heterogeneity: Methods and Protocols

Автор: Turksen Kursad
Название: Stem Cell Heterogeneity: Methods and Protocols
ISBN: 1493982346 ISBN-13(EAN): 9781493982349
Издательство: Springer
Рейтинг:
Цена: 15372.00 р.
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Описание: Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity.


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