In Vitro Models for Stem Cell Therapy: Methods and Protocols, Stock Peggy, Christ Bruno

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
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Цена: 5809.00 р.
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Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
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Цена: 2000.00 р.
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Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Автор: Proetzel Gabriele, Wiles Michael V.
Название: Mouse Models for Drug Discovery: Methods and Protocols
ISBN: 1493981099 ISBN-13(EAN): 9781493981090
Издательство: Springer
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Цена: 15372.00 р.
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Описание: With genetic engineering, systems explored in this book now exist allowing for the simple, efficient, and near universally precise genetic manipulation directly in any organism, including the mouse. Herein, these models are applied to a wide field of disease areas, including diabetes, cardiovascular disease, skin disorders, cancer, neurodegenerative and neuromuscular diseases, retinal disorders, as well as various behavioral models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and fully updated, Mouse Models for Drug Discovery: Methods and Protocols, Second Edition serves to equip the reader with an extensive overview of techniques to utilize the many possibilities of mice in the drug development process.

Автор: Brevini Tiziana A. L., Fazeli Alireza, Turksen Kursad
Название: Next Generation Culture Platforms for Reliable in Vitro Models: Methods and Protocols
ISBN: 107161245X ISBN-13(EAN): 9781071612453
Издательство: Springer
Цена: 18167.00 р.
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Описание: This detailed book collects original protocols aimed at encouraging and stimulating the scientific community to design and produce models for the laboratory that mimic cell guidance conditions as they occur in vivo.

Автор: Turksen Kursad
Название: Stem Cell Renewal and Cell-Cell Communication: Methods and Protocols
ISBN: 1071615696 ISBN-13(EAN): 9781071615690
Издательство: Springer
Цена: 16769.00 р.
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Описание:

1. Inference of Ligand-Receptor Pairs from Single-Cell Transcriptomics Data

Автор: Kidder Benjamin L.
Название: Stem Cell Transcriptional Networks: Methods and Protocols
ISBN: 1071603000 ISBN-13(EAN): 9781071603000
Издательство: Springer
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Цена: 16769.00 р.
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Описание: This second edition provides techniques used to study of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. Chapters detail next-generation sequencing technologies, data analysis, protocols on analysis and visualization of single-cell RNA-Seq data, analysis of 3D chromatin architecture, interpretation of large-scale interaction networks, transcriptional networks in embryonic and adult stem cells, derivation of stem cells, and transcriptional programs that promote reprogramming, transdifferentiation, and cancer formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Stem Cell Transcriptional Network: Methods and Protocols, Second Edition aims to provide a key resource for biologists seeking to interrogate these vital networks.

Автор: Turksen Kursad
Название: Stem Cell Nanotechnology: Methods and Protocols
ISBN: 1071603590 ISBN-13(EAN): 9781071603598
Издательство: Springer
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Цена: 15372.00 р.
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Описание: This fully updated edition brings together a new set of protocols to arm stem cell biologists with tools and approaches to continue the quest to uncover the intricacies and regulatory circuits underlying stem cell biology. The collection explores the tremendous progress in our understanding of the potential of stem cells and the regulatory mechanisms controlling their fate, particularly through the parallel advances in nanotechnologies and their applications in biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cell Nanotechnology: Methods and Protocols, Second Edition serves a valuable tool for experts and novices alike in the vital field of stem cell research.

Автор: Turksen Kursad
Название: Stem Cell Heterogeneity: Methods and Protocols
ISBN: 1493982346 ISBN-13(EAN): 9781493982349
Издательство: Springer
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Цена: 15372.00 р.
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Описание: Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity.

Автор: Ishikawa Kiyotake
Название: Cardiac Gene Therapy: Methods and Protocols
ISBN: 1493982435 ISBN-13(EAN): 9781493982431
Издательство: Springer
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Цена: 15372.00 р.
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Описание: Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets.

Автор: King Aileen J. F.
Название: Animal Models of Diabetes: Methods and Protocols
ISBN: 1071603841 ISBN-13(EAN): 9781071603840
Издательство: Springer
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Цена: 16769.00 р.
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Описание: This volume discusses a variety of animal models of diabetes, as well as describes techniques used to study end-points when using these models.

Автор: Westhead David R., Vijayabaskar M. S.
Название: Hidden Markov Models: Methods and Protocols
ISBN: 1493982923 ISBN-13(EAN): 9781493982929
Издательство: Springer
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Цена: 16070.00 р.
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Описание: This volume aims to provide a new perspective on the broader usage of Hidden Markov Models (HMMs) in biology.

Автор: Irving C. Allen (Editor)
Название: Mouse Models of Innate Immunity: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627034803 ISBN-13(EAN): 9781627034807
Издательство: Springer
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Цена: 20962.00 р.
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Описание: The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense;