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Glycosylation: Methods and Protocols, Davey Gavin


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Автор: Davey Gavin
Название:  Glycosylation: Methods and Protocols
ISBN: 9781071616840
Издательство: Springer
Классификация:



ISBN-10: 1071616846
Обложка/Формат: Hardcover
Страницы: 378
Вес: 0.73 кг.
Дата издания: 20.10.2021
Серия: Methods in molecular biology
Язык: English
Издание: 1st ed. 2022
Иллюстрации: 100 tables, color; 113 illustrations, color; xi, 332 p. 113 illus. in color.
Размер: 25.91 x 20.32 x 2.03 cm
Читательская аудитория: Professional & vocational
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: This volume explores the latest techniques and methods used for performing up-to-date glycosylation research. The chapters in this book are organized into four parts. Part One looks at the latest analytical and bioinformatics technologies that enable the characterization of glycosylation complexity. Part Two details the importance of synthetic chemistry and glycoengineering in the fields of bioprocessing and biotherapeutic development. Part Three discusses systems biology and computational technologies used by scientists to analyze glycosylation events in the cell. Part Four focuses on how cellular glycosylation biomarkers can be identified and used to characterize human clinical datasets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Glycosylation: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this exciting and developing field.
Дополнительное описание: Glycoinformatics Tools for Comprehensive Characterization of Glycans Enzymatically Released from Proteins.- GlycoStore: A Platform for H/UPLC and Capillary Electrophoresis Glycan Data.- An Interactive View of Glycosylation.- Characterization and Analysis



In Situ Detection of DNA Damage / Methods and Protocols

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
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Цена: 5809.00 р.
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Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Affinity Chromatography / Methods and Protocols

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
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Цена: 2000.00 р.
Наличие на складе: Есть (2 шт.)
Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Single Nucleotide Polymorphisms / Methods and Protocols

Автор: Kwok Pui-Yan
Название: Single Nucleotide Polymorphisms / Methods and Protocols
ISBN: 0896039684 ISBN-13(EAN): 9780896039681
Издательство: Springer
Цена: 5809.00 р.
Наличие на складе: Есть (2 шт.)
Описание: Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.

Extracellular RNA: Methods and Protocols

Автор: Patel Tushar
Название: Extracellular RNA: Methods and Protocols
ISBN: 1493976516 ISBN-13(EAN): 9781493976515
Издательство: Springer
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Цена: 11179.00 р.
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Описание:

1. Extracellular RNAs - A New Awareness of Old Perspectives

Noah Sadik, Lilian Cruz, Alessandra Gurtner, Rodosthenis S. Rodosthenous , Sophie A. Dusoswa, Olivia Ziegler, Thomas Sebastiaan Van Solinge, Zhiyun Wei, Ane Miren Salvador Garicano, Bence Gyorgy, Marike Broekman, and Leonora Balaj

2. Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles

Julia Small, Roger Alexander, and Leonora Balaj

3. Extracellular RNA Isolation from Cell Culture Supernatant

Sawen Bakr., Bridget Simonson, Kirsty M. Danielson, and Saumya Das

4. Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture

Irene K. Yan, Neha Shukla, David A. Borrelli, and Tushar Patel

5. Isolation of Extracellular RNA from Serum/Plasma

Justyna Filant, Parham Nejad, Anu Paul, Bridget Simonson, Srimeenakshi Srinivasan, Xuan Zhang, Leonora Balaj, Saumya Das, Roopali Gandhi, Louise C. Laurent, and Anil K. Sood

6. Isolation of Extracellular RNA from Bile

Irene K. Yan, Valentin X. Berdah, and Tushar Patel

7. Cushioned-Density Gradient Ultracentrifugation (C-DGUC): a Refined and High Performance Method for the Isolation, Characterization & Use of Exosomes

Kang Li, David K. Wong, King Yeung Hong, and Robert L. Raffai

8. Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids

Pete Heinzelman

9. Enrichment of Extracellular Vesicle Subpopulations via Affinity Chromatography

Michelle E. Hung, Stephen B. Lenzini, Devin M. Stranford, and Joshua N. Leonard

10. Detection and Analysis of Non-vesicular Extracellular RNA

Juan Pablo Tosar and Alfonso Cayota

11. Isolation of Plasma Lipoproteins as a Source of Extracellular RNA

Kang Li, David K. Wong, Fu Sang Luk, Roy Y. Kim, and Robert L. Raffai

12. Droplet Digital PCR for Quantitation of Extracellular RNA

Irene K Yan, Rishabh Lohray, and Tushar Patel

13. Preparation of Small RNA NGS Libraries from Biofluids

Alton Etheridge, Kai Wang, David Baxter, and David Galas

14. Multiplexed Detection and Quantitation of Extracellular Vesicle RNA Expression using NanoString

Neha Shukla, Irene K. Yan, and Tushar Patel

15. Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs

Akiko Matsuda and Tushar Patel

16. Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs

Marie-Cйcile Didiot, Reka A. Haraszti, Neil Aronin, and Anastasia Khvorova

Glycosylation

Автор: Mohammed Al-Rubeai
Название: Glycosylation
ISBN: 9048160685 ISBN-13(EAN): 9789048160686
Издательство: Springer
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Цена: 23508.00 р.
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Описание: This book provides a unique and up-to-date insight into the biopharmaceutical industry. Largely written by industrial authors, its scope is multidisciplinary, rendering it an ideal reference source for students undertaking advanced undergraduate or postgraduate courses in biotechnology, pharmaceutical science, biochemistry, or medicine.

Diabetes and Protein Glycosylation

Автор: Harold Rifkin; Margo Panush Cohen
Название: Diabetes and Protein Glycosylation
ISBN: 1461293669 ISBN-13(EAN): 9781461293668
Издательство: Springer
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Цена: 14673.00 р.
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Описание: In addition to reviewing a wealth of investigative possibilities in the area of these chronic complications-including eye, kidney, nerve, and vascular disease-Dr. Cohen indicates how enhanced nonenzymatic glycosylation in uncontrolled diabetes underscores the pressing need for maintenance of long-term euglycemia.

Protein Glycosylation

Автор: Roslyn M. Bill; Leigh Revers; Iain B.H. Wilson
Название: Protein Glycosylation
ISBN: 1461372410 ISBN-13(EAN): 9781461372417
Издательство: Springer
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Цена: 27950.00 р.
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Описание: Protein Glycosylation provides clear, up-to-date, and integrated coverage of key topics in this field.

The Role of Glycosylation in Health and Disease

Автор: Lauc Gordan, Trbojevic-Akmačic Irena
Название: The Role of Glycosylation in Health and Disease
ISBN: 303070114X ISBN-13(EAN): 9783030701147
Издательство: Springer
Цена: 27950.00 р.
Наличие на складе: Поставка под заказ.

Описание: Glycobiology is an emerging field of studying glycans (sugars) and glycoconjugates that are essentially involved in almost all biological processes, from fine-tuning glycoprotein function to protein-protein interactions, signaling, immune response, host-pathogen interactions, etc.

Glycosylation and Cancer,126

Автор: Richard R Drake
Название: Glycosylation and Cancer,126
ISBN: 0128013818 ISBN-13(EAN): 9780128013816
Издательство: Elsevier Science
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Цена: 19875.00 р.
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Описание:

Advances in Cancer Research provides invaluable information on the exciting and fast-moving field of cancer research. Here, once again, outstanding and original reviews are presented on a variety of topics.

Glycosylation Engineering of Biopharmaceuticals

Автор: Alain Beck
Название: Glycosylation Engineering of Biopharmaceuticals
ISBN: 1493962663 ISBN-13(EAN): 9781493962662
Издательство: Springer
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Цена: 16769.00 р.
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Описание: Glyco-engineering is being developed as a method to control the composition of carbohydrates and to enhance the pharmacological properties of monoclonal antibodies (mAbs) and other proteins. In Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols, experts in the field provide readers with production and characterization protocols of glycoproteins and glyco-engineered biopharmaceuticals with a focus on mAbs. The volume is divided in four complementary parts dealing with glyco-engineering of therapeutic proteins, glycoanalytics, glycoprotein complexes characterization, and PK/PD assays for therapeutic antibodies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols serves as an ideal guide for scientists striving to push forward the exciting field of engineered biopharmaceuticals.

Protein Glycosylation

Автор: Roslyn M. Bill; Leigh Revers; Iain B.H. Wilson
Название: Protein Glycosylation
ISBN: 0792383370 ISBN-13(EAN): 9780792383376
Издательство: Springer
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Цена: 36570.00 р.
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Описание: Presents an overview of the chemical structures of mono- and oligosaccharides. This book includes discussions on the purification, function, and enzyme kinetics of selected glycosidases and glycotransferases, as well as a review of the roles of oligosaccharides in glycoprotein function and the in vivo role of glycoproteins themselves.

Barley: Methods and Protocols

Автор: Wendy A. Harwood
Название: Barley: Methods and Protocols
ISBN: 1493989421 ISBN-13(EAN): 9781493989423
Издательство: Springer
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Цена: 27950.00 р.
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Описание: This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and easy-to-use, Barley: Methods and Protocols serves as a valuable reference volume for cereal researchers and breeders by providing detailed protocols covering important traditional skills such as crossing and tissue culture through to the latest technologies for genotyping, expression analysis, and genome editing.

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