Stem Cells and Tissue Repair: Methods and Protocols, Kioussi Chrissa
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Di Nardo Paolo, Dhingra Sanjiv, Singla Dinender K. Название: Adult Stem Cells: Methods and Protocols ISBN: 1493982931 ISBN-13(EAN): 9781493982936 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents... Contributing Authors...
1. A Simple Protocol to Isolate, Characterize and Expand Dental Pulp Stem Cells Federica Di Scipio, Andrea Elio Sprio, Maria Elisabetta Carere, Zhiqian Yang, and Giovanni Nicolao Berta 2. Derivation of Mesenchymal Stem cells from Embryonic Stem Cells: A Non-Variable and Inexhaustive Source of Adult Stem Cells Glen Lester Sequiera, Niketa Sareen, Ejlal Abu Al-Rub, and Sanjiv Dhingra 3. Compact Bone-Derived Multipotent Mesenchymal Stromal Cells (MSCs) for the Treatment of Sjogren's-Like Disease in NOD Mice Ghada Abu Elghanam, Younan Liu, Saeed Khalili, Dongdong Fang, and Simon D.Tran 4. Manipulating the Proliferative Potential of Cardiomyocytes by Gene Transfer Giulia Prosdocimo and Mauro Giacca 5. Assessment of Energy Metabolic Changes in Adipose Tissue-Derived Stem Cells Ghazaleh Hajmousa and Martin C. Harmsen 6. Isolation and In Vitro Characterization of Epidermal Stem Cells Kasper S. Moestrup, Marianne S. Andersen, and Kim B. Jensen 7. Endothelial Progenitor Cells: Procedure for Cell Isolation and Characterization Garikipati V.N. Srikanth, Zhongjian Cheng, and Raj Kishore 8. Therapeutic Application of Placental Mesenchymal Stem Cells Reprogrammed Neurospheres in Spinal Cord Injury of SCID Vikram Sabapathy, Franklin Jebaraj Herbert, and Sanjay Kumar 9. Therapeutic Application of Human Wharton Jelly Mesenchymal Stem Cells in Skin Injury of SCID Vikram Sabapathy, Balasubramanian Sundaram, and Sanjay Kumar 10. Isolation, Characterization, and Expansion of Cancer Stem Cells Luke A. Torre-Healy, Artem Berezovsky, and Justin D. Lathia 11. Cardiac Progenitor Cell Isolation from Human Auricles Paolo Di Nardo and Francesca Pagliari 12. Isolation and Culture of Satellite Cells from Mouse Skeletal Muscle Antonio Musarт and Silvia Carosio 13. Isolation of Stromal Stem Cells from Adipose Tissue Maria Prat, Francesca Oltolina, Silvia Antonini, and Andrea Zamperone 14. Human Induced Pluripotent Stem Cell-Derived Mesencymal Stem Cells as an Individual-Specific and Renewable Source of Adult Stem Cells15. A Simplified and Systematic Method to Isolate, Culture and Characterize Multiple Types of Human Dental Stem Cells from a Single Tooth Mohammed Bakkar, Younan Liu, Dongdong Fang, Camille Stegen, Xinyun Su, Murali Ramamoorthi, Li-Chieh Lin, Takako Kawasaki, Nicholas Makhoul, Huan Pham, Yoshinori Sumita, and Simon D. Tran 16. Measurement of Autophagy by Flow Cytometry Silvia Zappavigna, Angela Lombardi, Gabriella Misso, Anna Grimaldi, and Michele Caraglia 17. Echocardiography-Guided Intramyocardial Injection Method in a Murine Model Kay Maeda, Rick Seymour, Marc Ruel, and Erik J. Suuronen 18. Noninvasive Assessment of Cell Fate and Biology in Transplanted Mesenchymal Stem Cells Federico Franchi and Martin Rodriguez-Porcel 19. Methods for Long Term Storage of Murine Bone Marrow Derived Mesenchymal Stem Cells Niketa Sareen, Ejlal Abu-El-Rub, Glen Lester Sequiera, Meenal Moudgil, and Sanjiv Dhingra 20. Therapeutic Application of Adult Stem Cells in the Heart Taylor A. Johnson and Dinender K. Singla 21. Patient-Derived and Intra-Operatively Formed Biomaterial for Tissue Engineering Shalmli U. Joshi, Rares O. Barbu, Melissa Carr-Reynolds, Brian Barnes, and Syam P. Nukavarapu 22. Three-Dimensional Printed Scaffolds with Multipotent Mesenchymal Stromal Cells for Rabbit Mandibular Reconstruction and Engineering Dongdong Fang, Michael Roskies, Mohamed-Nur Abdallah, Mohammed Bakkar, Jack Jordan, Li-Chieh Lin, Faleh Tamimi, and Simon D. Tran 23. Optimal Enviro
5. High-Efficiency Transfection of Glioblastoma Cells and a Simple Spheroid Migration Assay
Carsten Hagemann, Diana Amend, Almuth F. Kessler, Thomas Linsenmann, Ralf-Ingo Ernestus, and Mario Lцhr
6. Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells
Xiaoxing Cheng and Rui Jian
7. Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cells
Soizik Berlivet, Isabelle Hmitou, Hйlиne Picaud, and Matthieu Gйrard
8. Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8
Takayuki Manabe and Akio Wanaka
9. A Recessive Genetic Screen for Components of the RNA Interference Pathway Performed in Mouse Embryonic Stem Cells
Melanie I. Trombly and Xiaozhong Wang
10. RNAi Knockdown of Ape1 Gene in the Differentiation of Mouse Embryonic Stem Cells
Gang-Ming Zou, Jieqing Yu, Cynthia LeBron, and Yumei Fu
11. An Efficient Transfection Method for Differentiation and Cell Proliferation of Mouse Embryonic Stem Cells
Jun-Yang Liou, Bor-Sheng Ko, and Tzu-Ching Chang
12. Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetry
Mario Lцhr, Thomas Linsenmann, Anna Jawork, Almuth F. Kessler, Nils Timmermann, Gyцrgy A. Homola, Ralf-Ingo Ernestus, and Carsten Hagemann
13. RNAi-Based Techniques for the Analysis of Gene Function in Drosophila Germline Stem Cells
Amelia J. Helms, Danielle S. Finger, Victoria L. Hardy, and Elizabeth T. Ables
14. In Vivo RNAi in the Drosophila Follicular Epithelium: Analysis of Stem Cell Maintenance, Proliferation, and Differentiation
Veit Riechmann
15. A Phenotype-Based RNAi Screening for Ras-ERK/MAPK Signaling-Associated Stem Cell Regulators in C. elegans
Myon-Hee Lee and Dong Suk Yoon
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Автор: Perdiguero Eusebio, Cornelison Ddw Название: Muscle Stem Cells: Methods and Protocols ISBN: 1493982966 ISBN-13(EAN): 9781493982967 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides leading-edge protocols in the study of the molecular and cellular biology of muscle stem cells.
Автор: Cobaleda Cйsar, Sбnchez-Garcнa Isidro Название: Leukemia Stem Cells: Methods and Protocols ISBN: 1071608096 ISBN-13(EAN): 9781071608098 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The detailed volume aims to provide a comprehensive hands-on manual covering all the techniques involved in the cellular and molecular identification and characterization of both normal hematopoietic and leukemic stem cells, both from human patients and from mouse models of human leukemia.
1. Introduction to Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Robert M. Hoffman 2. Nestin-Based Reporter Transgenic Mouse Lines John Mignone, Natalia Peunova, and Grigori Enikolopov 3. Discovery of HAP Stem Cells Lingna Li and Robert M. Hoffman 4. Peripheral-Nerve and Spinal-Cord Regeneration in Mice Using Hair Follicle-Associated-Pluripotent (HAP) Stem Cells Yasuyuki Amoh, Kensei Katsuoka, and Robert M. Hoffman 5. Construction of Tissue Engineered Nerve Conduits Seeded with Neurons Derived from Hair Follicle Neural Crest Stem Cells Fang Liu, Haiyan Lin, and Chuansen Zhang 6.Nestin-Expressing Hair Follicle-Associated Pluripotent (HAP) Stem Cells Promote Whisker Sensory-Nerve Growth in Long-Term 3D-Gelfoam Histoculture Sumiyuki Mii, Jennifer Duong, Yasunori Tome, Aisada Uchugonova, Fang Liu, Yasuyuki Amoh, Norimitsu Saito, Kensei Katsuoka, and Robert M. Hoffman 7. Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles Ruifeng Yang and Xiaowei Xu
8. Isolation of Mouse Hair Follicle Bulge Stem Cells and their Functional Analysis in a Reconstitution Assay Ying Zheng, Jen-Chih Hsieh, Julia Escandon, and George Cotsarelis 9.Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle Germ Katsunari Tezuka, Koh-ei Toyoshima, and Takashi Tsuji 10. Hair Induction by Cultured Mesenchymal Cells Using Sphere Formation Kazuo Kishi and Ruka Hayashi 11. Stereological Quantification of Cell Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing Eduardo Martнnez-Martнnez, Eileen Uribe-Querol, Claudio I. Galvбn-Hernбndez, and Gabriel Gutiйrrez-Ospina 12. Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size-Determination Nicholas W. Rufaut, Allan J. Nixon, and Rodney D. Sinclair 13. Isolation and Fluorescence Activated Cell-Sorting of Mouse Keratinocytes Expressing β-Galactosidase Maria Kasper, Rune Toftgеrd, and Viljar Jaks 14. Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cord of Mice Wenluo Cao, Fang Liu, Yasuyuki Amoh, and Robert M. Hoffman
15. Long-Term Gelfoam(R) Histoculture of Hair-Shaft-Producing Mouse Whisker Follicles Wenluo Cao, Fang Liu, Yasuyuki Amoh, and Robert M. Hoffman 16. From Hair to Heart: Hair Follicle-Associated-Pluripotent (HAP) Stem Cells Differentiate to Beating Cardiac Muscle Cells Masateru Yashiro, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara, Robert M. Hoffman, and Yasuyuki Amoh 17.Highly Efficient Neural Differentiation of CD34 Positive Hair Follicle-Associated Pluripotent (HAP) Stem Cells Induced by Retinoic Acid and Serum Free Medium Mohsen Sagha and Nowruz Najafzadeh 18. Cryopreservation of Intact Hair Follicle Maintains Pluripotency of Nestin-Expressing (HAP) Stem Cells Satoshi Kajiura, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara, Lingna Li, Kensei Katsuoka, Robert M. Hoffman, and Yasuyuki Amoh
Автор: Gigante Margherita, Ranieri Elena Название: Cytotoxic T-Cells: Methods and Protocols ISBN: 1071615068 ISBN-13(EAN): 9781071615065 Издательство: Springer Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Flow Cytometry Instrument Setting as a Crucial Checkpoint for Optimal T-Cell Analysis and Sorting
Achille Anselmo and Federico Simone Colombo
2. Phenotypical and Functional Characterization of Cytotoxic Unconventional T-Cells
Margherita Gigante and Elena Ranieri
3. Tumor Infiltrating T-Cell Cytotoxicity Assay
Nada Chaoul and Marcello Albanesi
4. Antigen-Specific In Vivo Killing Assay
Nada Chaoul and Gilles Dadaglio
5. CTL ELISPOT Assay and T-Cell Detection
Elena Ranieri, Giuseppe Stefano Netti, and Margherita Gigante
6. Methods for Characterization of Senescent Circulating and Tumor-Infiltrating T-Cells: An Overview from Multicolor Flow Cytometry to Single Cell-RNA Sequencing
Rossana Franzin, Alessandra Stasi, Giuseppe Castellano, and Loreto Gesualdo
7. In Vivo and In Vitro Models for the Study of Bone Remodeling and the Role of Immune Cells
Giacomina Brunetti and Maria Grano
8. Characterization of Cytotoxic T Lymphocytes (CTL) by Tissue Microarray
Giuseppe Stefano Netti, Monica Rutigliano, Chiara Divella, and Giuseppe Lucarelli
9. A Review of Proteomics Strategies to Study T-Cells Activation and Function in Cancer Disease
Massimo Papale
10. Methods to Study Post-Translational Modification Patterns in Cytotoxic T-Cells and Cancer
Annamaria Deleonardis and Massimo Papale
11. Whole Exome Sequencing for the Identification of Mutations in Cd8+T-Cells
Sharon Natasha Cox
12. Handling High-Throughput Omics Data for Systems Genetics Analysis
Francesco Pesce and Paolo Protopapa
13. Analysis of miRNA Expression Using Digital PCR and the QuantStudio(TM) 3D Digital PCR System
Francesca Conserva, Loreto Gesualdo, and Paola Pontrelli
14. Cytotoxic T Lymphocytes (CTLs) and Kidney Transplantation: An Overview
Federica Rascio, Paola Pontrelli, and Giuseppe Grandaliano
15. Protein-Bound Uremic Toxins and Immunity
Maria Teresa Rocchetti, Carmela Cosola, Elena Ranieri, and Loreto Gesualdo
16. Gut Microbiota, the Immune System, and Cytotoxic T Lymphocytes
Carmela Cosola, Maria Teresa Rocchetti, and Loreto Gesualdo
Автор: Mion Francesca, Tonon Silvia Название: Regulatory B Cells: Methods and Protocols ISBN: 1071612360 ISBN-13(EAN): 9781071612361 Издательство: Springer Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.
Описание: This detailed book explores the utilization and delivery of stem cells for therapeutic purposes in patients in the clinic and the tightly controlled Good Manufacturing Practices (GMP) that make these powerful techniques possible.
Автор: Turksen Kursad Название: Epidermal Cells: Methods and Protocols ISBN: 1071602500 ISBN-13(EAN): 9781071602508 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Поставка под заказ.
Описание: This fully updated edition brings together a new set of protocols to arm epidermal cell biologists with tools and approaches to continue the quest to uncover the intricacies and regulatory mechanisms underlying epidermal cell development and function.
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