Rice Genome Engineering and Gene Editing: Methods and Protocols, Bandyopadhyay Anindya, Thilmony Roger
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 2000.00 р. Наличие на складе: Есть (2 шт.) Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 5809.00 р. Наличие на складе: Есть (4 шт.) Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Описание: This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.
Автор: Hatada Izuho Название: Genome Editing in Animals: Methods and Protocols ISBN: 1493983938 ISBN-13(EAN): 9781493983933 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Construction and Evaluation of Zinc Finger Nucleases
Hiroshi Ochiai and Takashi Yamamoto
2. Current Overview of TALEN Construction Systems
Tetsushi Sakuma and Takashi Yamamoto
3. CRISPR/Cas9
Izuho Hatada and Takuro Horii
4. Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-Target Risks in Human and Mouse
Qingbo Wang and Kumiko Ui-Tei
5. Genome Editing of Mouse by Cytoplasmic Injection
Takuro Horii and Izuho Hatada
6. Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing sgRNA/Cas9 Expressing Plasmids
Taichi Noda, Asami Oji, and Masahito Ikawa
7. Genome Editing in Mouse and Rat by Electroporation
Takehito Kaneko
8. Generation of Knock-in Mouse by Genome Editing
Wataru Fujii
9. Genome Editing of Rat
Takehito Kaneko
10. Gene Targeting in Rabbits: Single-step Generation of Knockout Rabbits by Microinjection of CRISPR/Cas9 Plasmids
Yoshihiro Kawano and Arata Honda
11. Genome Editing of Pig
Masahito Watanabe and Hiroshi Nagashima
12. Genome Editing of Monkey
Zhen Liu, Yijun Cai, and Qiang Sun
13. Genome Editing Mediated by Primordial Germ Cell in Chicken
Jae Yong Han and Hong Jo Lee
14. CRISPR/Cas9-mediated Targeted Knock-in of Exogenous Reporter Genes in Zebrafish
Atsuo Kawahara
15. Genome Editing of Medaka
Satoshi Ansai and Masato Kinoshita
16. A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders using the CRISPR-Cas System
Yuto Sakane, Ken-ich T Suzuki, and Takashi Yamamoto
17. Genome Editing of Silkworms
Takuya Tsubota and Hideki Sezutsu
18. Genome Editing in the Cricket, Gryllus bimaculatus
Takahito Watanabe, Sumihare Noji, and Taro Mito
19. Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease
Yasunori Sasakura, Keita Yoshida, and Nicholas Treen
20. &
Автор: Aphasizhev Ruslan Название: RNA and DNA Editing: Methods and Protocols ISBN: 1493958216 ISBN-13(EAN): 9781493958214 Издательство: Springer Рейтинг: Цена: 16769.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The recent expansion in diversity of RNA and DNA editing types has stimulated the development of many unique genetic, molecular, biochemical, and computational approaches to biological issues. In RNA and DNA Editing: Methods and Protocols, leading experts in the field introduce methods developed over the last few years to study editing substrates, mechanisms of specificity, and functions of RNA and DNA editing enzymes and complexes. Sections of the book are dedicated to state-of-the art techniques which enable investigation of uracil insertion/deletion RNA editing in mitochondrion of Trypanosoma brucei, adenosine to inosine RNA editing, cytidine to uracil RNA and DNA editing, as well as tRNA editing and RNA modifications. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA and DNA Editing: Methods and Protocols seeks to inspire the further development of these vital and powerful techniques.
Автор: Lee Kiho Название: Zygotic Genome Activation: Methods and Protocols ISBN: 1493983598 ISBN-13(EAN): 9781493983599 Издательство: Springer Рейтинг: Цена: 19564.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA.
Автор: Kaufmann Kerstin, Mueller-Roeber Bernd Название: Plant Gene Regulatory Networks: Methods and Protocols ISBN: 149398392X ISBN-13(EAN): 9781493983926 Издательство: Springer Рейтинг: Цена: 22359.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
Preface... Table of Contents...
Contributing Authors...
1. From Genes to Networks: Characterizing Gene-Regulatory Interactions in Plants
Kerstin Kaufmann and Dijun Chen
Part I Experimental Approaches to Study Plant Gene-Regulatory Networks
2. Inducible Promoter Systems for Gene Perturbation Experiments in Arabidopsis Bennett Thomson, Emmanuelle Graciet, and Frank Wellmer
3. Cell Type-Specific Gene Expression Profiling Using Fluorescence-Activated Nuclear Sorting Daniel Slane and Martin Bayer
4. Characterization of Cell Type-Specific DNA Binding Sites of Plant Transcription Factors using Chromatin Immunoprecipitation On Sun Lau
5. Yeast One and Two Hybrid High-Throughput Screenings using Arrayed Libraries Rocнo Sбnchez-Montesino and Luis Oсate-Sбnchez
6.SELEX-Seq: A Method to Determine DNA-Binding Specificities of Plant Transcription Factors Cezary Smaczniak, Gerco C. Angenent and Kerstin Kaufmann
7. Analysis of a Plant Transcriptional Regulatory Network using Transient Expression Systems Sara Dнaz-Triviсo, Yuchen Long, Ben Scheres, and Ikram Blilou
8.Analysis of In Vivo Chromatin and Protein Interactions of Arabidopsis Transcript Elongation Factors Alexander Pfab, Wojciech Antosz, Philipp Holzinger, Astrid Bruckmann, Joachim Griesenbeck, and Klaus D. Grasser
9.Characterization of Mediator Complex and its Associated Proteins from Rice Subhasis Samanta and Jitendra Kumar Thakur
10. DNase I SIM: A Simplified In-Nucleus Method for DNase I Hypersensitive Site Sequencing Sergei A. Filichkin and Molly Megraw
11. In Situ Hi-C Library Preparation for Plants to Study their Three-Dimensional Chromatin Interactions on a Genome-Wide Scale Chang Liu
12. Multiplexed Transcriptional Activation or Repression in Plants using CRISPR-dCas9 Based Systems Levi G. Lowder, Joseph W. Paul III, and Yiping Qi
13. Generation of dTALEs and Libraries of Synthetic TALE-Activated Promoters for Engineering of Gene Regulatory Networks in Plants Tom Schreiber and Alain Tissier
Part II Computational Approaches to Study Plant Gene-Regulatory Networks
14.Design of Knowledge Bases for Plant Gene Regulatory Networks Eric Mukundi, Fabio Gomez-Cano, Wilberforce Zachary Ouma, and Erich Grotewold
15. AraNet: A Network Biology Server for Arabidopsis thaliana and Other Non-Model Plant Species Tak Lee and Insuk Lee
16. Integration of Genome-Wide TF Binding and Gene Expression Data to Characterize Gene Regulatory Networks in Plant Development Dijun Chen and Kerstin Kaufmann
17. Predicting Transcription Factor-Binding Sites and their Cognate Transcription Factors using Gene Expression Data Chun-Ping Yu and Wen-Hsiung Li
18. Computational Approaches to Study Gene Regulatory Networks Nooshin Omranian and Zoran Nikoloski
19. Boolean Dynamic Modeling Approaches to Study Plant Gene Regulatory Networks: Integration, Validation and Prediction J. Davila-Velderrain, J.C. Martinez-Garcia, and R.E. Alvarez Buylla
20. ODE-Based Modeling of Complex Regulatory Circuits Daniel D. Seaton
21. Inferring Gene Regulatory Networks in the Arabidopsis Root using a Dynamic Bayesian Network Approach Maria Ang
Автор: Grutzmann Robert, Pilarsky Christian Название: Cancer Gene Profiling: Methods and Protocols ISBN: 1493979973 ISBN-13(EAN): 9781493979974 Издательство: Springer Рейтинг: Цена: 15998.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:
1. Factors Affecting the Use of Human Tissues in Biomedical Research: Implications in the Design and Operation of a Biorepository Daniel S. Atherton, Katherine C. Sexton, Dennis Otali, Walter C. Bell, and William E. Grizzle
2. Tissue Microdissection Anja Rabien and Glen Kristiansen
3. Tissue Microarrays Ana-Maria Dancau, Ronald Simon, Martina Mirlacher, and Guido Sauter
4. Gene Expression Analysis in the Age of Mass Sequencing: An Introduction Christian Pilarsky, Lahiri Kanth Nanduri, and Janine Roy
5. Quantitative DNA Methylation in Cancer Ole Ammerpohl, Andrea Haake, Julia Kolarova, and Reiner Siebert
6. Single Nucleotide Polymorphism to Associate Cancer Risk Victoria Shaw, Katie Bullock, and William Greenhalf
7: Exosomes in Cancer Disease Margot Zцller
8: MicroRNAs (miRNAs) Profiling Lu Gao and Feng Jiang
9. Cancer Gene Profiling for Response Prediction B. Michael Ghadimi and Peter Jo
10. Target Gene Discovery for Novel Therapeutic Agents in Cancer Treatment Sanjay Tiwari, Ole Ammerpohl, and Holger Kalthoff
11. Application of Proteomics in Cancer Biomarker Discovery: GeLC-MS/MS Pedro R. Cutillas and Tatjana Crnogorac-Jurcevic
12. Meta-Analysis of Cancer Gene Profiling Data Janine Roy, Christof Winter, and Michael Schroeder
13. Reproducible, Scalable Fusion Gene Detection from RNA-Seq Vladan Arsenijevic and Brandi N. Davis-Dusenbery
14. Transcriptome Sequencing for the Detection of Chimeric Transcripts Hsueh-Ting Chu
Автор: Burgess-Brown Nicola A. Название: Heterologous Gene Expression in E.Coli: Methods and Protocols ISBN: 1493983288 ISBN-13(EAN): 9781493983285 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E.
Автор: Ishikawa Kiyotake Название: Cardiac Gene Therapy: Methods and Protocols ISBN: 1493982435 ISBN-13(EAN): 9781493982431 Издательство: Springer Рейтинг: Цена: 15372.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets.
Автор: Minczuk Michal, Rorbach Joanna Название: Mitochondrial Gene Expression: Methods and Protocols ISBN: 1071608339 ISBN-13(EAN): 9781071608333 Издательство: Springer Рейтинг: Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Authoritative and cutting-edge, Mitochondrial Gene Expression: Methods and Protocols aims to provide complementary approaches and practical guidelines. Chapter 7 is available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Автор: Soucek Laura, Whitfield Jonathan Название: The Myc Gene: Methods and Protocols ISBN: 1071614754 ISBN-13(EAN): 9781071614754 Издательство: Springer Цена: 18167.00 р. Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: 1. An "-omycs" Toolbox to Work with MYC
Jonathan R. Whitfieldand Laura Soucek
2. Methods of Expression, Purification and Preparation of the c-Myc b-HLH-LZ for its Biophysical Characterization Patrick Delattre, Martin Montagne, and Pierre Lavigne
3. Biophysical and Structural Methods to Study the bHLHzip Region of Human c-MYC
Giovanna Zinzalla
4. Identifying and Validating MYC: protein Interactors in Pursuit of Novel anti-MYC Therapies
Diana Resetca, Alannah S. MacDonald, Tristan M. G. Kenney, Yong Wei, Cheryl H. Arrowsmith, Brian Raught, and Linda Z. Penn
5. Detection of Post-Translational Modifications on MYC
Colin J. Daniel, Xiao-Xin Sun, Yingxiao Chen, Xiaoli Zhang, Mu-Shui Dai, and Rosalie C. Sears
6. MYC Analysis in Cancer and Evolution
Markus Hartl and Klaus Bister
7. Genome-wide analysis of c-MYC-regulated mRNAs and miRNAs, and c-MYC DNA-binding by Next Generation Sequencing
Rene Jackstadt, Markus Kaller, Antje Menssen, and Heiko Hermeking
8. Analysis of Myc Chromatin Binding by Calibrated ChIP-seq Approach
Donald P. Cameron, Vladislav Kuzin, and Laura Baranello
9. A High-Throughput Chromatin Immunoprecipitation Sequencing Approach to Study the Role of MYC on the Epigenetic Landscape
Luca Fagnocchiand Alessio Zippo
10. Methods for Determining Myc-induced Apoptosis
Dan Lu, Catherine Wilson, and Trevor D. Littlewood
11. Measuring MYC-mediated Metabolism in Tumorigenesis
Hsin-Yao Tang, Aaron R. Goldman, Xue Zhang, David W. Speicher, and Chi V. Dang
12. Methods to study Myc-regulated Cellular Senescence - An Update
Fan Zhang, Wesam Bazzar, Mohammad Alzrigat, and Lars-Gunnar Larsson
13. Examining Myc-Dependent Translation Changes in Cellular Homeostasis and Cancer
Joanna R. Kovalski, Yichen Xu, and Davide Ruggero
14. A Versatile in vivo System to Study Myc in Cell Reprogramming
Elena Senis, Lluc Mosteiro, Dirk Grimm, and Maria Abad
15. Using Flow Cytometry to Study Myc's Role in Shaping the Tumor Immune Microenvironment
Sнlvia Casacuberta-Serra
16. Generation of a Tetracycline Regulated Mouse Model of MYC-Induced
T-Cell Acute Lymphoblastic Leukemia
Wadie D. Mahauad-Fernandez, Kavya Rakhra, and Dean W. Felsher
17. Chromogenic inSitu Hybridization (CISH) as a Method for Detection of C-Myc Amplification in Formalin-Fixed Paraffin-Embedded Tumor Tissue: An Update
Natasa Todorovic-Rakovic
18. MYC Copy Number Detection in Clinical Samples using a Digital DNA-Hybridization and Detection Method
Zighereda Ogbah, Francesco Mattia Mancuso, and Ana Vivancos
19. Cell-Based Methods for the Identification of Myc-Inhibitory Small Molecules
Catherine A. Burkhart, Michelle Haber, Murray D. Norris, Andrei V. Gudkov, and Mikhail A. Nikiforov
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