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Synthetic Gene Circuits: Methods and Protocols, Menolascina Filippo

Menolascina Filippo Synthetic Gene Circuits: Methods and Protocols 9781071610312

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Автор: Menolascina Filippo
Название: Synthetic Gene Circuits: Methods and Protocols
ISBN: 9781071610312
Издательство: Springer
Классификация:

Медицинская генетика

Научное оборудование, научные эксперименты и методы

Биология и науки о жизни

Биотехнологии

ISBN-10: 1071610317
Обложка/Формат: Hardcover
Страницы: 353
Вес: 0.77 кг.
Дата издания: 07.02.2021
Язык: English
Размер: 26.16 x 19.56 x 2.29 cm
Ссылка на Издательство: Link
Поставляется из: Германии
Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.

The art of electronics The x Chapters

Автор: Horowitz Paul
Название: The art of electronics The x Chapters
ISBN: 1108499945 ISBN-13(EAN): 9781108499941
Издательство: Cambridge Academ
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Цена: 8870.00 р.
Наличие на складе: Заказано в издательстве.

Описание: The x-Chapters is the missing pieces of The Art of Electronics, to be used either as its complement, or as a direct route to exploring some of the most exciting and oft-overlooked topics in advanced electronic engineering. Students, researchers and practitioners will find here techniques and circuits that are unavailable elsewhere.

In Situ Detection of DNA Damage / Methods and Protocols

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
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Цена: 5809.00 р.
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Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Affinity Chromatography / Methods and Protocols

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
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Цена: 2000.00 р.
Наличие на складе: Есть (2 шт.)
Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Plant Synthetic Promoters: Methods and Protocols

Автор: Hehl Reinhard
Название: Plant Synthetic Promoters: Methods and Protocols
ISBN: 1493981862 ISBN-13(EAN): 9781493981861
Издательство: Springer
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Цена: 15372.00 р.
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Описание: Practical and cutting-edge, Plant Synthetic Promoters: Methods and Protocols covers all steps required from the identification of cis-regulatory elements, over synthetic promoter design, to the experimental analysis of synthetic promoter function.

Synthetic Protein Switches: Methods and Protocols

Автор: Stein Viktor
Название: Synthetic Protein Switches: Methods and Protocols
ISBN: 1493983458 ISBN-13(EAN): 9781493983452
Издательство: Springer
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Цена: 16769.00 р.
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Описание: Beginning with general strategies, the volume continues with coverage of peptide switches, fluorescent and bioluminescent sensors, ss-lactamase sensors, proteolytic sensors, optogenetic switches, as well as cellular signaling switches.

Synthetic Antibodies: Methods and Protocols

Автор: Tiller Thomas
Название: Synthetic Antibodies: Methods and Protocols
ISBN: 1493983199 ISBN-13(EAN): 9781493983193
Издательство: Springer
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Цена: 16769.00 р.
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Описание: This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs).

Cardiac Gene Therapy: Methods and Protocols

Автор: Ishikawa Kiyotake
Название: Cardiac Gene Therapy: Methods and Protocols
ISBN: 1493982435 ISBN-13(EAN): 9781493982431
Издательство: Springer
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Цена: 15372.00 р.
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Описание: Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets.

Mitochondrial Gene Expression: Methods and Protocols

Автор: Minczuk Michal, Rorbach Joanna
Название: Mitochondrial Gene Expression: Methods and Protocols
ISBN: 1071608339 ISBN-13(EAN): 9781071608333
Издательство: Springer
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Цена: 18167.00 р.
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Описание: Authoritative and cutting-edge, Mitochondrial Gene Expression: Methods and Protocols aims to provide complementary approaches and practical guidelines. Chapter 7 is available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Plant Gene Regulatory Networks: Methods and Protocols

Автор: Kaufmann Kerstin, Mueller-Roeber Bernd
Название: Plant Gene Regulatory Networks: Methods and Protocols
ISBN: 149398392X ISBN-13(EAN): 9781493983926
Издательство: Springer
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Цена: 22359.00 р.
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Описание:

Preface...
Table of Contents...

Contributing Authors...

1. From Genes to Networks: Characterizing Gene-Regulatory Interactions in Plants

Kerstin Kaufmann and Dijun Chen

Part I Experimental Approaches to Study Plant Gene-Regulatory Networks

2. Inducible Promoter Systems for Gene Perturbation Experiments in Arabidopsis
Bennett Thomson, Emmanuelle Graciet, and Frank Wellmer

3. Cell Type-Specific Gene Expression Profiling Using Fluorescence-Activated Nuclear Sorting
Daniel Slane and Martin Bayer

4. Characterization of Cell Type-Specific DNA Binding Sites of Plant Transcription Factors using Chromatin Immunoprecipitation
On Sun Lau

5. Yeast One and Two Hybrid High-Throughput Screenings using Arrayed Libraries
Rocнo Sбnchez-Montesino and Luis Oсate-Sбnchez

6. SELEX-Seq: A Method to Determine DNA-Binding Specificities of Plant Transcription Factors
Cezary Smaczniak, Gerco C. Angenent and Kerstin Kaufmann

7. Analysis of a Plant Transcriptional Regulatory Network using Transient Expression Systems
Sara Dнaz-Triviсo, Yuchen Long, Ben Scheres, and Ikram Blilou

8. Analysis of In Vivo Chromatin and Protein Interactions of Arabidopsis Transcript Elongation Factors
Alexander Pfab, Wojciech Antosz, Philipp Holzinger, Astrid Bruckmann, Joachim Griesenbeck, and Klaus D. Grasser

9. Characterization of Mediator Complex and its Associated Proteins from Rice
Subhasis Samanta and Jitendra Kumar Thakur

10. DNase I SIM: A Simplified In-Nucleus Method for DNase I Hypersensitive Site Sequencing
Sergei A. Filichkin and Molly Megraw

11. In Situ Hi-C Library Preparation for Plants to Study their Three-Dimensional Chromatin Interactions on a Genome-Wide Scale
Chang Liu

12. Multiplexed Transcriptional Activation or Repression in Plants using CRISPR-dCas9 Based Systems
Levi G. Lowder, Joseph W. Paul III, and Yiping Qi

13. Generation of dTALEs and Libraries of Synthetic TALE-Activated Promoters for Engineering of Gene Regulatory Networks in Plants
Tom Schreiber and Alain Tissier

Part II Computational Approaches to Study Plant Gene-Regulatory Networks

14. Design of Knowledge Bases for Plant Gene Regulatory Networks
Eric Mukundi, Fabio Gomez-Cano, Wilberforce Zachary Ouma, and Erich Grotewold

15. AraNet: A Network Biology Server for Arabidopsis thaliana and Other Non-Model Plant Species
Tak Lee and Insuk Lee

16. Integration of Genome-Wide TF Binding and Gene Expression Data to Characterize Gene Regulatory Networks in Plant Development
Dijun Chen and Kerstin Kaufmann

17. Predicting Transcription Factor-Binding Sites and their Cognate Transcription Factors using Gene Expression Data
Chun-Ping Yu and Wen-Hsiung Li

18. Computational Approaches to Study Gene Regulatory Networks
Nooshin Omranian and Zoran Nikoloski

19. Boolean Dynamic Modeling Approaches to Study Plant Gene Regulatory Networks: Integration, Validation and Prediction
J. Davila-Velderrain, J.C. Martinez-Garcia, and R.E. Alvarez Buylla

20. ODE-Based Modeling of Complex Regulatory Circuits
Daniel D. Seaton

21. Inferring Gene Regulatory Networks in the Arabidopsis Root using a Dynamic Bayesian Network Approach
Maria Ang

Heterologous Gene Expression in E.Coli: Methods and Protocols

Автор: Burgess-Brown Nicola A.
Название: Heterologous Gene Expression in E.Coli: Methods and Protocols
ISBN: 1493983288 ISBN-13(EAN): 9781493983285
Издательство: Springer
Рейтинг:
Цена: 18167.00 р.
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Описание: This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E.

The Myc Gene: Methods and Protocols

Автор: Soucek Laura, Whitfield Jonathan
Название: The Myc Gene: Methods and Protocols
ISBN: 1071614754 ISBN-13(EAN): 9781071614754
Издательство: Springer
Цена: 18167.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: 1. An "-omycs" Toolbox to Work with MYC

Jonathan R. Whitfieldand Laura Soucek

2. Methods of Expression, Purification and Preparation of the c-Myc b-HLH-LZ for its Biophysical Characterization Patrick Delattre, Martin Montagne, and Pierre Lavigne

3. Biophysical and Structural Methods to Study the bHLHzip Region of Human c-MYC

Giovanna Zinzalla

4. Identifying and Validating MYC: protein Interactors in Pursuit of Novel anti-MYC Therapies

Diana Resetca, Alannah S. MacDonald, Tristan M. G. Kenney, Yong Wei, Cheryl H. Arrowsmith, Brian Raught, and Linda Z. Penn

5. Detection of Post-Translational Modifications on MYC

Colin J. Daniel, Xiao-Xin Sun, Yingxiao Chen, Xiaoli Zhang, Mu-Shui Dai, and Rosalie C. Sears

6. MYC Analysis in Cancer and Evolution

Markus Hartl and Klaus Bister

7. Genome-wide analysis of c-MYC-regulated mRNAs and miRNAs, and c-MYC DNA-binding by Next Generation Sequencing

Rene Jackstadt, Markus Kaller, Antje Menssen, and Heiko Hermeking

8. Analysis of Myc Chromatin Binding by Calibrated ChIP-seq Approach

Donald P. Cameron, Vladislav Kuzin, and Laura Baranello

9. A High-Throughput Chromatin Immunoprecipitation Sequencing Approach to Study the Role of MYC on the Epigenetic Landscape

Luca Fagnocchiand Alessio Zippo

10. Methods for Determining Myc-induced Apoptosis

Dan Lu, Catherine Wilson, and Trevor D. Littlewood

11. Measuring MYC-mediated Metabolism in Tumorigenesis

Hsin-Yao Tang, Aaron R. Goldman, Xue Zhang, David W. Speicher, and Chi V. Dang

12. Methods to study Myc-regulated Cellular Senescence - An Update

Fan Zhang, Wesam Bazzar, Mohammad Alzrigat, and Lars-Gunnar Larsson

13. Examining Myc-Dependent Translation Changes in Cellular Homeostasis and Cancer

Joanna R. Kovalski, Yichen Xu, and Davide Ruggero

14. A Versatile in vivo System to Study Myc in Cell Reprogramming

Elena Senis, Lluc Mosteiro, Dirk Grimm, and Maria Abad

15. Using Flow Cytometry to Study Myc's Role in Shaping the Tumor Immune Microenvironment

Sнlvia Casacuberta-Serra

16. Generation of a Tetracycline Regulated Mouse Model of MYC-Induced

T-Cell Acute Lymphoblastic Leukemia

Wadie D. Mahauad-Fernandez, Kavya Rakhra, and Dean W. Felsher

17. Chromogenic in Situ Hybridization (CISH) as a Method for Detection of C-Myc Amplification in Formalin-Fixed Paraffin-Embedded Tumor Tissue: An Update

Natasa Todorovic-Rakovic

18. MYC Copy Number Detection in Clinical Samples using a Digital DNA-Hybridization and Detection Method

Zighereda Ogbah, Francesco Mattia Mancuso, and Ana Vivancos

19. Cell-Based Methods for the Identification of Myc-Inhibitory Small Molecules

Catherine A. Burkhart, Michelle Haber, Murray D. Norris, Andrei V. Gudkov, and Mikhail A. Nikiforov

Rice Genome Engineering and Gene Editing: Methods and Protocols

Автор: Bandyopadhyay Anindya, Thilmony Roger
Название: Rice Genome Engineering and Gene Editing: Methods and Protocols
ISBN: 1071610678 ISBN-13(EAN): 9781071610671
Издательство: Springer
Цена: 18167.00 р.
Наличие на складе: Есть у поставщика Поставка под заказ.

Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.

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